LOCUS       sequence-125797-        4016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human ETS1 [NM_005238.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4016)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E064 Hs.ETS1-nostop
FEATURES             Location/Qualifiers
     source          1..4016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1507..1606)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1983..2774
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2867..3455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3356..3375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3609..3626
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3785..3812)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3904..3990)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgaa ggcggccgtc gatctcaagc cgactctcac catcatcaag acggaaaaag
      241 tcgatctgga gcttttcccc tccccggata tggaatgtgc agatgtccca ctattaactc
      301 caagcagcaa agaaatgatg tctcaagcat taaaagctac tttcagtggt ttcactaaag
      361 aacagcaacg actggggatc ccaaaagacc cccggcagtg gacagaaacc catgttcggg
      421 actgggtgat gtgggctgtg aatgaattca gcctgaaagg tgtagacttc cagaagttct
      481 gtatgaatgg agcagccctc tgcgccctgg gtaaagactg ctttctcgag ctggccccag
      541 actttgttgg ggacatctta tgggaacatc tagagatcct gcagaaagag gatgtgaaac
      601 catatcaagt taatggagtc aacccagcct atccagaatc ccgctatacc tcggattact
      661 tcattagcta tggtattgag catgcccagt gtgttccacc atcggagttc tcagagccca
      721 gcttcatcac agagtcctat cagacgctcc atcccatcag ctcggaagag ctcctctccc
      781 tcaagtatga gaatgactac ccctcggtca ttctccgaga ccctctccag acagacacct
      841 tgcagaatga ctactttgct atcaaacaag aagtcgtcac cccagacaac atgtgcatgg
      901 ggaggaccag tcgtggtaaa ctcgggggcc aggactcttt tgaaagcata gagagctacg
      961 atagttgtga tcgcctcacc cagtcctgga gcagccagtc atctttcaac agcctgcagc
     1021 gtgttccctc ctatgacagc ttcgactcag aggactatcc ggctgccctg cccaaccaca
     1081 agcccaaggg caccttcaag gactatgtgc gggaccgtgc tgacctcaat aaggacaagc
     1141 ctgtcattcc tgctgctgcc ctagctggct acacaggcag tggaccaatc cagctatggc
     1201 agtttcttct ggaattactc actgataaat cctgtcagtc ttttatcagc tggacaggag
     1261 atggctggga attcaaactt tctgacccag atgaggtggc caggagatgg ggaaagagga
     1321 aaaacaaacc taagatgaat tatgagaaac tgagccgtgg cctacgctac tattacgaca
     1381 aaaacatcat ccacaagaca gcggggaaac gctacgtgta ccgctttgtg tgtgacctgc
     1441 agagcctgct ggggtacacc cctgaggagc tgcacgccat gctggacgtc aagccagatg
     1501 ccgacgagtc aactttcttg tacaaagttg gcattatagg aaagcattgc ttatcaattt
     1561 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgcatg caagcttaga
     1621 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1681 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1741 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1801 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1861 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1921 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1981 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2041 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2101 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2161 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2221 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2281 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2341 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2401 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2461 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2521 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2581 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2641 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2701 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2761 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     2821 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2881 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2941 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3001 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3061 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3121 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3181 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3241 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3301 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3361 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3421 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3481 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3541 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3601 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3661 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3721 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3781 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3841 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3901 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3961 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcg
//
