LOCUS       sequence-125810-        5108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human EXOC6 [NM_019053.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5108)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E077 Hs.EXOC6
FEATURES             Location/Qualifiers
     source          1..5108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2599..2698)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3075..3866
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3959..4547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4448..4467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4701..4718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4877..4904)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4996..5082)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc ggagaacagc gagagtctgg gcaccgtccc cgagcacgag cggatcttgc
      241 aggagatcga gagcaccgac accgcctgtg tggggcccac cctccggtct gtgtatgatg
      301 accaaccaaa tgcgcacaag aagtttatgg aaaagttaga tgcttgtatc cgtaatcatg
      361 acaaggaaat tgaaaagatg tgtaattttc atcatcaggg ttttgtagat gctattacag
      421 aactccttaa agtaaggact gatgcagaaa aactgaaggt gcaagttact gataccaacc
      481 gaaggtttca agatgctgga aaagaggtga tagtccacac agaagatatc attcgatgta
      541 gaattcagca gagaaatatt acaactgtag tagaaaaatt gcagttatgc cttcctgtgc
      601 tagaaatgta cagtaagctg aaagaacaga tgagtgccaa aaggtactat tctgccctaa
      661 aaactatgga acaattagag aatgtgtact ttccctgggt tagtcaatac cggttttgtc
      721 agctcatgat agaaaatctt cccaaactcc gtgaggatat taaagaaatc tccatgtctg
      781 atctcaaaga ctttttggaa agtattcgaa aacattctga caaaataggt gaaacagcaa
      841 tgaaacaggc acagcatcag aaaaccttca gtgtttctct gcagaaacaa aataaaatga
      901 aatttgggaa aaatatgtat ataaatcgtg atagaattcc agaggaaagg aatgaaactg
      961 tattgaaaca ttcacttgaa gaagaggatg agaatgaaga agagatctta actgttcagg
     1021 atcttgttga tttttcccct gtttatcgat gtttgcacat ttattctgtt ttgggtgacg
     1081 aggaaacatt tgaaaactat tatcgaaaac aaagaaagaa acaagcaaga ctggtattgc
     1141 aaccccagtc gaatatgcat gaaacagttg atggctatag aagatatttc actcaaattg
     1201 tagggttctt tgtggtagaa gatcacattt tacatgtgac ccaaggatta gtaaccaggg
     1261 catacactga tgaactttgg aacatggccc tctcaaagat aattgctgtc cttagagctc
     1321 attcatccta ttgcactgat cctgatcttg ttctggagct gaagaatctt actgtaatat
     1381 ttgcagatac tttacagggt tatggttttc cagtgaaccg actttttgac cttttatttg
     1441 aaataagaga ccaatacaat gaaacactgc ttaagaaatg ggctggagtt ttcagggaca
     1501 tttttgaaga agataattac agccccatcc ctgttgtcaa tgaagaagaa tataaaattg
     1561 tcatcagcaa atttcccttt caagatccag accttgaaaa gcagtctttc ccaaagaaat
     1621 tccccatgtc tcagtcagtg cctcatattt acattcaagt taaagaattt atttatgcca
     1681 gccttaaatt ttcagagtca ctacaccgga gctcaacaga aatagacgat atgcttagaa
     1741 aatcaacaaa tctgctgctg accagaactt tgagtagctg tttactgaac cttattagaa
     1801 aacctcatat aggtttgaca gagctggtac aaatcatcat aaacacaaca cacctggagc
     1861 aagcttgtaa atatcttgag gactttataa ctaacattac aaatatttcc caagaaactg
     1921 ttcatactac aagactttat ggactttcta ctttcaagga tgctcgacat gcagcagaag
     1981 gagaaatata taccaaactg aatcaaaaaa ttgatgaatt tgttcagctt gctgattatg
     2041 actggacaat gtctgagcca gatggaagag ctagtggtta tttaatggac cttataaatt
     2101 ttttgagaag catctttcaa gtgtttactc atttgcctgg gaaagttgct cagacagctt
     2161 gcatgtcagc ctgccagcat ctgtcaacat ccttaatgca gatgctactg gacagtgagt
     2221 taaaacaaat aagcatggga gctgttcagc agtttaactt agatgtcata cagtgtgaat
     2281 tgtttgccag ctctgagcct gtgccaggat tccaggggga taccctgcag ctagcattca
     2341 ttgacctcag acaactcctt gacctgttta tggtttggga ttggtctact tacctagctg
     2401 attatgggca gccagcttct aagtaccttc gggtgaatcc aaacacagcc cttactcttt
     2461 tggagaagat gaaggatact agcaaaaaga acaatatatt tgctcagttc aggaagaatg
     2521 atcgagacaa acagaagttg atagagacag tcgtgaaaca gctgagaagt ttggtgaatg
     2581 gtatgtccca gcacatgtaa tcaactttct tgtacaaagt tggcattata ggaaagcatt
     2641 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca
     2701 tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     2761 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2821 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2881 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2941 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     3001 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     3061 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     3121 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     3181 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     3241 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     3301 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     3361 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     3421 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     3481 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     3541 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     3601 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3661 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3721 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3781 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3841 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3901 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3961 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4021 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4081 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4141 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4201 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4261 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4321 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     4381 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4441 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4501 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4561 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4621 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4681 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4741 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4801 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4861 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4921 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4981 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     5041 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     5101 ctctcgcg
//
