LOCUS       sequence-125811-        5105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human EXOC6 [NM_019053.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5105)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E078 Hs.EXOC6-nostop
FEATURES             Location/Qualifiers
     source          1..5105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2596..2695)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3072..3863
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3956..4544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4445..4464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4698..4715
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4874..4901)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4993..5079)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc ggagaacagc gagagtctgg gcaccgtccc cgagcacgag cggatcttgc
      241 aggagatcga gagcaccgac accgcctgtg tggggcccac cctccggtct gtgtatgatg
      301 accaaccaaa tgcgcacaag aagtttatgg aaaagttaga tgcttgtatc cgtaatcatg
      361 acaaggaaat tgaaaagatg tgtaattttc atcatcaggg ttttgtagat gctattacag
      421 aactccttaa agtaaggact gatgcagaaa aactgaaggt gcaagttact gataccaacc
      481 gaaggtttca agatgctgga aaagaggtga tagtccacac agaagatatc attcgatgta
      541 gaattcagca gagaaatatt acaactgtag tagaaaaatt gcagttatgc cttcctgtgc
      601 tagaaatgta cagtaagctg aaagaacaga tgagtgccaa aaggtactat tctgccctaa
      661 aaactatgga acaattagag aatgtgtact ttccctgggt tagtcaatac cggttttgtc
      721 agctcatgat agaaaatctt cccaaactcc gtgaggatat taaagaaatc tccatgtctg
      781 atctcaaaga ctttttggaa agtattcgaa aacattctga caaaataggt gaaacagcaa
      841 tgaaacaggc acagcatcag aaaaccttca gtgtttctct gcagaaacaa aataaaatga
      901 aatttgggaa aaatatgtat ataaatcgtg atagaattcc agaggaaagg aatgaaactg
      961 tattgaaaca ttcacttgaa gaagaggatg agaatgaaga agagatctta actgttcagg
     1021 atcttgttga tttttcccct gtttatcgat gtttgcacat ttattctgtt ttgggtgacg
     1081 aggaaacatt tgaaaactat tatcgaaaac aaagaaagaa acaagcaaga ctggtattgc
     1141 aaccccagtc gaatatgcat gaaacagttg atggctatag aagatatttc actcaaattg
     1201 tagggttctt tgtggtagaa gatcacattt tacatgtgac ccaaggatta gtaaccaggg
     1261 catacactga tgaactttgg aacatggccc tctcaaagat aattgctgtc cttagagctc
     1321 attcatccta ttgcactgat cctgatcttg ttctggagct gaagaatctt actgtaatat
     1381 ttgcagatac tttacagggt tatggttttc cagtgaaccg actttttgac cttttatttg
     1441 aaataagaga ccaatacaat gaaacactgc ttaagaaatg ggctggagtt ttcagggaca
     1501 tttttgaaga agataattac agccccatcc ctgttgtcaa tgaagaagaa tataaaattg
     1561 tcatcagcaa atttcccttt caagatccag accttgaaaa gcagtctttc ccaaagaaat
     1621 tccccatgtc tcagtcagtg cctcatattt acattcaagt taaagaattt atttatgcca
     1681 gccttaaatt ttcagagtca ctacaccgga gctcaacaga aatagacgat atgcttagaa
     1741 aatcaacaaa tctgctgctg accagaactt tgagtagctg tttactgaac cttattagaa
     1801 aacctcatat aggtttgaca gagctggtac aaatcatcat aaacacaaca cacctggagc
     1861 aagcttgtaa atatcttgag gactttataa ctaacattac aaatatttcc caagaaactg
     1921 ttcatactac aagactttat ggactttcta ctttcaagga tgctcgacat gcagcagaag
     1981 gagaaatata taccaaactg aatcaaaaaa ttgatgaatt tgttcagctt gctgattatg
     2041 actggacaat gtctgagcca gatggaagag ctagtggtta tttaatggac cttataaatt
     2101 ttttgagaag catctttcaa gtgtttactc atttgcctgg gaaagttgct cagacagctt
     2161 gcatgtcagc ctgccagcat ctgtcaacat ccttaatgca gatgctactg gacagtgagt
     2221 taaaacaaat aagcatggga gctgttcagc agtttaactt agatgtcata cagtgtgaat
     2281 tgtttgccag ctctgagcct gtgccaggat tccaggggga taccctgcag ctagcattca
     2341 ttgacctcag acaactcctt gacctgttta tggtttggga ttggtctact tacctagctg
     2401 attatgggca gccagcttct aagtaccttc gggtgaatcc aaacacagcc cttactcttt
     2461 tggagaagat gaaggatact agcaaaaaga acaatatatt tgctcagttc aggaagaatg
     2521 atcgagacaa acagaagttg atagagacag tcgtgaaaca gctgagaagt ttggtgaatg
     2581 gtatgtccca gcacatgtca actttcttgt acaaagttgg cattatagga aagcattgct
     2641 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgcatgc
     2701 aagcttagat cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     2761 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     2821 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2881 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2941 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     3001 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     3061 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     3121 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     3181 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     3241 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     3301 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     3361 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     3421 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     3481 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     3541 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     3601 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     3661 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     3721 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3781 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3841 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3901 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3961 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4021 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4081 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4141 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4201 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4261 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4321 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     4381 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4441 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4501 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4561 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4621 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4681 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4741 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4801 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     4861 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     4921 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     4981 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     5041 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     5101 tcgcg
//
