LOCUS       sequence-125816-        5156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human FGFR1 [NM_001174063.1] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5156)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E083 Hs.FGFR1
FEATURES             Location/Qualifiers
     source          1..5156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(565..582)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(2647..2746)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3123..3914
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4007..4595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4496..4515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4749..4766
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4925..4952)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5044..5130)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtg gagctggaag tgcctcctct tctgggctgt gctggtcaca gccacactct
      241 gcaccgctag gccgtccccg accttgcctg aacaagccca gccctgggga gcccctgtgg
      301 aagtggagtc cttcctggtc caccccggtg acctgctgca gcttcgctgt cggctgcggg
      361 acgatgtgca gagcatcaac tggctgcggg acggggtgca gctggcggaa agcaaccgca
      421 cccgcatcac aggggaggag gtggaggtgc aggactccgt gcccgcagac tccggcctct
      481 atgcttgcgt aaccagcagc ccctcgggca gtgacaccac ctacttctcc gtcaatgttt
      541 cagatgctct cccctcctcg gaggatgatg atgatgatga tgactcctct tcagaggaga
      601 aagaaacaga taacaccaaa ccaaaccgta tgcccgtagc tccatattgg acatccccag
      661 aaaagatgga aaagaaattg catgcagtgc cggctgccaa gacagtgaag ttcaaatgcc
      721 cttccagtgg gaccccaaac cccacactgc gctggttgaa aaatggcaaa gaattcaaac
      781 ctgaccacag aattggaggc tacaaggtcc gttatgccac ctggagcatc ataatggact
      841 ctgtggtgcc ctctgacaag ggcaactaca cctgcattgt ggagaatgag tacggcagca
      901 tcaaccacac ataccagctg gatgtcgtgg agcggtcccc tcaccggccc atcctgcaag
      961 cagggttgcc cgccaacaaa acagtggccc tgggtagcaa cgtggagttc atgtgtaagg
     1021 tgtacagtga cccgcagccg cacatccagt ggctaaagca catcgaggtg aatgggagca
     1081 agattggccc agacaacctg ccttatgtcc agatcttgaa gactgctgga gttaatacca
     1141 ccgacaaaga gatggaggtg cttcacttaa gaaatgtctc ctttgaggac gcaggggagt
     1201 atacgtgctt ggcgggtaac tctatcggac tctcccatca ctctgcatgg ttgaccgttc
     1261 tggaagccct ggaagagagg ccggcagtga tgacctcgcc cctgtacctg gagatcatca
     1321 tctattgcac aggggccttc ctcatctcct gcatggtggg gtcggtcatc gtctacaaga
     1381 tgaagagtgg taccaagaag agtgacttcc acagccagat ggctgtgcac aagctggcca
     1441 agagcatccc tctgcgcaga caggtgtctg ctgactccag tgcatccatg aactctgggg
     1501 ttcttctggt tcggccatca cggctctcct ccagtgggac tcccatgcta gcaggggtct
     1561 ctgagtatga gcttcccgaa gaccctcgct gggagctgcc tcgggacaga ctggtcttag
     1621 gcaaacccct gggagagggc tgctttgggc aggtggtgtt ggcagaggct atcgggctgg
     1681 acaaggacaa acccaaccgt gtgaccaaag tggctgtgaa gatgttgaag tcggacgcaa
     1741 cagagaaaga cttgtcagac ctgatctcag aaatggagat gatgaagatg atcgggaagc
     1801 ataagaatat catcaacctg ctgggggcct gcacgcagga tggtcccttg tatgtcatcg
     1861 tggagtatgc ctccaagggc aacctgcggg agtacctgca ggcccggagg cccccagggc
     1921 tggaatactg ctacaacccc agccacaacc cagaggagca gctctcctcc aaggacctgg
     1981 tgtcctgcgc ctaccaggtg gcccgaggca tggagtatct ggcctccaag aagtgcatac
     2041 accgagacct ggcagccagg aatgtcctgg tgacagagga caatgtgatg aagatagcag
     2101 actttggcct cgcacgggac attcaccaca tcgactacta taaaaagaca accaacggcc
     2161 gactgcctgt gaagtggatg gcacccgagg cattatttga ccggatctac acccaccaga
     2221 gtgatgtgtg gtctttcggg gtgctcctgt gggagatctt cactctgggc ggctccccat
     2281 accccggtgt gcctgtggag gaacttttca agctgctgaa ggagggtcac cgcatggaca
     2341 agcccagtaa ctgcaccaac gagctgtaca tgatgatgcg ggactgctgg catgcagtgc
     2401 cctcacagag acccaccttc aagcagctgg tggaagacct ggaccgcatc gtggccttga
     2461 cctccaacca ggagtacctg gacctgtcca tgcccctgga ccagtactcc cccagctttc
     2521 ccgacacccg gagctctacg tgctcctcag gggaggattc cgtcttctct catgagccgc
     2581 tgcccgagga gccctgcctg ccccgacacc cagcccagct tgccaatggc ggactcaaac
     2641 gccgctaatc aactttcttg tacaaagttg gcattatagg aaagcattgc ttatcaattt
     2701 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgcatg caagcttaga
     2761 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     2821 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     2881 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2941 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     3001 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     3061 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     3121 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     3181 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     3241 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     3301 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     3361 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     3421 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     3481 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     3541 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     3601 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     3661 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     3721 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     3781 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     3841 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     3901 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     3961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4141 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4381 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4441 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4621 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4681 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4741 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     4801 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4861 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     4921 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     4981 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     5041 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     5101 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcg
//
