LOCUS       sequence-125817-        5153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human FGFR1 [NM_001174063.1] without stop codon
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5153)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E084 Hs.FGFR1-nostop
FEATURES             Location/Qualifiers
     source          1..5153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(565..582)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(2644..2743)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3120..3911
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4004..4592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4493..4512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4746..4763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4922..4949)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5041..5127)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtg gagctggaag tgcctcctct tctgggctgt gctggtcaca gccacactct
      241 gcaccgctag gccgtccccg accttgcctg aacaagccca gccctgggga gcccctgtgg
      301 aagtggagtc cttcctggtc caccccggtg acctgctgca gcttcgctgt cggctgcggg
      361 acgatgtgca gagcatcaac tggctgcggg acggggtgca gctggcggaa agcaaccgca
      421 cccgcatcac aggggaggag gtggaggtgc aggactccgt gcccgcagac tccggcctct
      481 atgcttgcgt aaccagcagc ccctcgggca gtgacaccac ctacttctcc gtcaatgttt
      541 cagatgctct cccctcctcg gaggatgatg atgatgatga tgactcctct tcagaggaga
      601 aagaaacaga taacaccaaa ccaaaccgta tgcccgtagc tccatattgg acatccccag
      661 aaaagatgga aaagaaattg catgcagtgc cggctgccaa gacagtgaag ttcaaatgcc
      721 cttccagtgg gaccccaaac cccacactgc gctggttgaa aaatggcaaa gaattcaaac
      781 ctgaccacag aattggaggc tacaaggtcc gttatgccac ctggagcatc ataatggact
      841 ctgtggtgcc ctctgacaag ggcaactaca cctgcattgt ggagaatgag tacggcagca
      901 tcaaccacac ataccagctg gatgtcgtgg agcggtcccc tcaccggccc atcctgcaag
      961 cagggttgcc cgccaacaaa acagtggccc tgggtagcaa cgtggagttc atgtgtaagg
     1021 tgtacagtga cccgcagccg cacatccagt ggctaaagca catcgaggtg aatgggagca
     1081 agattggccc agacaacctg ccttatgtcc agatcttgaa gactgctgga gttaatacca
     1141 ccgacaaaga gatggaggtg cttcacttaa gaaatgtctc ctttgaggac gcaggggagt
     1201 atacgtgctt ggcgggtaac tctatcggac tctcccatca ctctgcatgg ttgaccgttc
     1261 tggaagccct ggaagagagg ccggcagtga tgacctcgcc cctgtacctg gagatcatca
     1321 tctattgcac aggggccttc ctcatctcct gcatggtggg gtcggtcatc gtctacaaga
     1381 tgaagagtgg taccaagaag agtgacttcc acagccagat ggctgtgcac aagctggcca
     1441 agagcatccc tctgcgcaga caggtgtctg ctgactccag tgcatccatg aactctgggg
     1501 ttcttctggt tcggccatca cggctctcct ccagtgggac tcccatgcta gcaggggtct
     1561 ctgagtatga gcttcccgaa gaccctcgct gggagctgcc tcgggacaga ctggtcttag
     1621 gcaaacccct gggagagggc tgctttgggc aggtggtgtt ggcagaggct atcgggctgg
     1681 acaaggacaa acccaaccgt gtgaccaaag tggctgtgaa gatgttgaag tcggacgcaa
     1741 cagagaaaga cttgtcagac ctgatctcag aaatggagat gatgaagatg atcgggaagc
     1801 ataagaatat catcaacctg ctgggggcct gcacgcagga tggtcccttg tatgtcatcg
     1861 tggagtatgc ctccaagggc aacctgcggg agtacctgca ggcccggagg cccccagggc
     1921 tggaatactg ctacaacccc agccacaacc cagaggagca gctctcctcc aaggacctgg
     1981 tgtcctgcgc ctaccaggtg gcccgaggca tggagtatct ggcctccaag aagtgcatac
     2041 accgagacct ggcagccagg aatgtcctgg tgacagagga caatgtgatg aagatagcag
     2101 actttggcct cgcacgggac attcaccaca tcgactacta taaaaagaca accaacggcc
     2161 gactgcctgt gaagtggatg gcacccgagg cattatttga ccggatctac acccaccaga
     2221 gtgatgtgtg gtctttcggg gtgctcctgt gggagatctt cactctgggc ggctccccat
     2281 accccggtgt gcctgtggag gaacttttca agctgctgaa ggagggtcac cgcatggaca
     2341 agcccagtaa ctgcaccaac gagctgtaca tgatgatgcg ggactgctgg catgcagtgc
     2401 cctcacagag acccaccttc aagcagctgg tggaagacct ggaccgcatc gtggccttga
     2461 cctccaacca ggagtacctg gacctgtcca tgcccctgga ccagtactcc cccagctttc
     2521 ccgacacccg gagctctacg tgctcctcag gggaggattc cgtcttctct catgagccgc
     2581 tgcccgagga gccctgcctg ccccgacacc cagcccagct tgccaatggc ggactcaaac
     2641 gccgctcaac tttcttgtac aaagttggca ttataggaaa gcattgctta tcaatttgtt
     2701 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgcatgcaa gcttagatcc
     2761 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     2821 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     2881 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     2941 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     3001 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     3061 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     3121 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     3181 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     3241 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     3301 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     3361 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     3421 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     3481 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     3541 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     3601 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     3661 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     3721 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     3781 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     3841 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     3901 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     3961 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4021 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4081 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4141 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4201 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4261 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4321 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4381 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4441 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4561 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4621 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4681 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4741 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4801 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4861 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     4921 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     4981 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     5041 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     5101 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcg
//
