LOCUS       sequence-125820-        5114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human FGFR3 [NM_000142.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5114)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E087 Hs.FGFR3
FEATURES             Location/Qualifiers
     source          1..5114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2605..2704)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3081..3872
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3965..4553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4454..4473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4707..4724
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4883..4910)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5002..5088)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggg cgcccctgcc tgcgccctcg cgctctgcgt ggccgtggcc atcgtggccg
      241 gcgcctcctc ggagtccttg gggacggagc agcgcgtcgt ggggcgagcg gcagaagtcc
      301 cgggcccaga gcccggccag caggagcagt tggtcttcgg cagcggggat gctgtggagc
      361 tgagctgtcc cccgcccggg ggtggtccca tggggcccac tgtctgggtc aaggatggca
      421 cagggctggt gccctcggag cgtgtcctgg tggggcccca gcggctgcag gtgctgaatg
      481 cctcccacga ggactccggg gcctacagct gccggcagcg gctcacgcag cgcgtactgt
      541 gccacttcag tgtgcgggtg acagacgctc catcctcggg agatgacgaa gacggggagg
      601 acgaggctga ggacacaggt gtggacacag gggcccctta ctggacacgg cccgagcgga
      661 tggacaagaa gctgctggcc gtgccggccg ccaacaccgt ccgcttccgc tgcccagccg
      721 ctggcaaccc cactccctcc atctcctggc tgaagaacgg cagggagttc cgcggcgagc
      781 accgcattgg aggcatcaag ctgcggcatc agcagtggag cctggtcatg gaaagcgtgg
      841 tgccctcgga ccgcggcaac tacacctgcg tcgtggagaa caagtttggc agcatccggc
      901 agacgtacac gctggacgtg ctggagcgct ccccgcaccg gcccatcctg caggcggggc
      961 tgccggccaa ccagacggcg gtgctgggca gcgacgtgga gttccactgc aaggtgtaca
     1021 gtgacgcaca gccccacatc cagtggctca agcacgtgga ggtgaacggc agcaaggtgg
     1081 gcccggacgg cacaccctac gttaccgtgc tcaagacggc gggcgctaac accaccgaca
     1141 aggagctaga ggttctctcc ttgcacaacg tcacctttga ggacgccggg gagtacacct
     1201 gcctggcggg caattctatt gggttttctc atcactctgc gtggctggtg gtgctgccag
     1261 ccgaggagga gctggtggag gctgacgagg cgggcagtgt gtatgcaggc atcctcagct
     1321 acggggtggg cttcttcctg ttcatcctgg tggtggcggc tgtgacgctc tgccgcctgc
     1381 gcagcccccc caagaaaggc ctgggctccc ccaccgtgca caagatctcc cgcttcccgc
     1441 tcaagcgaca ggtgtccctg gagtccaacg cgtccatgag ctccaacaca ccactggtgc
     1501 gcatcgcaag gctgtcctca ggggagggcc ccacgctggc caatgtctcc gagctcgagc
     1561 tgcctgccga ccccaaatgg gagctgtctc gggcccggct gaccctgggc aagccccttg
     1621 gggagggctg cttcggccag gtggtcatgg cggaggccat cggcattgac aaggaccggg
     1681 ccgccaagcc tgtcaccgta gccgtgaaga tgctgaaaga cgatgccact gacaaggacc
     1741 tgtcggacct ggtgtctgag atggagatga tgaagatgat cgggaaacac aaaaacatca
     1801 tcaacctgct gggcgcctgc acgcagggcg ggcccctgta cgtgctggtg gagtacgcgg
     1861 ccaagggtaa cctgcgggag tttctgcggg cgcggcggcc cccgggcctg gactactcct
     1921 tcgacacctg caagccgccc gaggagcagc tcaccttcaa ggacctggtg tcctgtgcct
     1981 accaggtggc ccggggcatg gagtacttgg cctcccagaa gtgcatccac agggacctgg
     2041 ctgcccgcaa tgtgctggtg accgaggaca acgtgatgaa gatcgcagac ttcgggctgg
     2101 cccgggacgt gcacaacctc gactactaca agaagacaac caacggccgg ctgcccgtga
     2161 agtggatggc gcctgaggcc ttgtttgacc gagtctacac tcaccagagt gacgtctggt
     2221 cctttggggt cctgctctgg gagatcttca cgctgggggg ctccccgtac cccggcatcc
     2281 ctgtggagga gctcttcaag ctgctgaagg agggccaccg catggacaag cccgccaact
     2341 gcacacacga cctgtacatg atcatgcggg agtgctggca tgccgcgccc tcccagaggc
     2401 ccaccttcaa gcagctggtg gaggacctgg accgtgtcct taccgtgacg tccaccgacg
     2461 agtacctgga cctgtcggcg cctttcgagc agtactcccc gggtggccag gacaccccca
     2521 gctccagctc ctcaggggac gactccgtgt ttgcccacga cctgctgccc ccggccccac
     2581 ccagcagtgg gggctcgcgg acgtaatcaa ctttcttgta caaagttggc attataggaa
     2641 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2701 tttgcatgca agcttagatc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2761 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2821 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     2881 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2941 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     3001 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     3061 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     3121 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     3181 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     3241 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3301 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3361 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3421 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3481 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3541 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3601 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3661 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3721 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3781 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3841 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3901 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3961 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4021 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4081 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4141 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4201 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4261 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4321 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     4381 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4441 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4501 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4561 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4621 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4681 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4741 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4801 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     4861 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     4921 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     4981 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     5041 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     5101 tccctactct cgcg
//
