LOCUS       sequence-125821-        5111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human FGFR3 [NM_000142.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5111)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E088 Hs.FGFR3-nostop
FEATURES             Location/Qualifiers
     source          1..5111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2602..2701)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3078..3869
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3962..4550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4451..4470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4704..4721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4880..4907)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4999..5085)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggg cgcccctgcc tgcgccctcg cgctctgcgt ggccgtggcc atcgtggccg
      241 gcgcctcctc ggagtccttg gggacggagc agcgcgtcgt ggggcgagcg gcagaagtcc
      301 cgggcccaga gcccggccag caggagcagt tggtcttcgg cagcggggat gctgtggagc
      361 tgagctgtcc cccgcccggg ggtggtccca tggggcccac tgtctgggtc aaggatggca
      421 cagggctggt gccctcggag cgtgtcctgg tggggcccca gcggctgcag gtgctgaatg
      481 cctcccacga ggactccggg gcctacagct gccggcagcg gctcacgcag cgcgtactgt
      541 gccacttcag tgtgcgggtg acagacgctc catcctcggg agatgacgaa gacggggagg
      601 acgaggctga ggacacaggt gtggacacag gggcccctta ctggacacgg cccgagcgga
      661 tggacaagaa gctgctggcc gtgccggccg ccaacaccgt ccgcttccgc tgcccagccg
      721 ctggcaaccc cactccctcc atctcctggc tgaagaacgg cagggagttc cgcggcgagc
      781 accgcattgg aggcatcaag ctgcggcatc agcagtggag cctggtcatg gaaagcgtgg
      841 tgccctcgga ccgcggcaac tacacctgcg tcgtggagaa caagtttggc agcatccggc
      901 agacgtacac gctggacgtg ctggagcgct ccccgcaccg gcccatcctg caggcggggc
      961 tgccggccaa ccagacggcg gtgctgggca gcgacgtgga gttccactgc aaggtgtaca
     1021 gtgacgcaca gccccacatc cagtggctca agcacgtgga ggtgaatggc agcaaggtgg
     1081 gcccggacgg cacaccctac gttaccgtgc tcaagacggc gggcgctaac accaccgaca
     1141 aggagctaga ggttctctcc ttgcacaacg tcacctttga ggacgccggg gagtacacct
     1201 gcctggcggg caattctatt gggttttctc atcactctgc gtggctggtg gtgctgccag
     1261 ccgaggagga gctggtggag gctgacgagg cgggcagtgt gtatgcaggc atcctcagct
     1321 acggggtggg cttcttcctg ttcatcctgg tggtggcggc tgtgacgctc tgccgcctgc
     1381 gcagcccccc caagaaaggc ctgggctccc ccaccgtgca caagatctcc cgcttcccgc
     1441 tcaagcgaca ggtgtccctg gagtccaacg cgtccatgag ctccaacaca ccactggtgc
     1501 gcatcgcaag gctgtcctca ggggagggcc ccacgctggc caatgtctcc gagctcgagc
     1561 tgcctgccga ccccaaatgg gagctgtctc gggcccggct gaccctgggc aagccccttg
     1621 gggagggctg cttcggccag gtggtcatgg cggaggccat cggcattgac aaggaccggg
     1681 ccgccaagcc tgtcaccgta gccgtgaaga tgctgaaaga cgatgccact gacaaggacc
     1741 tgtcggacct ggtgtctgag atggagatga tgaagatgat cgggaaacac aaaaacatca
     1801 tcaacctgct gggcgcctgc acgcagggcg ggcccctgta cgtgctggtg gagtacgcgg
     1861 ccaagggtaa cctgcgggag tttctgcggg cgcggcggcc cccgggcctg gactactcct
     1921 tcgacacctg caagccgccc gaggagcagc tcaccttcaa ggacctggtg tcctgtgcct
     1981 accaggtggc ccggggcatg gagtacttgg cctcccagaa gtgcatccac agggacctgg
     2041 ctgcccgcaa tgtgctggtg accgaggaca acgtgatgaa gatcgcagac ttcgggctgg
     2101 cccgggacgt gcacaacctc gactactaca agaagacaac caacggccgg ctgcccgtga
     2161 agtggatggc gcctgaggcc ttgtttgacc gagtctacac tcaccagagt gacgtctggt
     2221 cctttggggt cctgctctgg gagatcttca cgctgggggg ctccccgtac cccggcatcc
     2281 ctgtggagga gctcttcaag ctgctgaagg agggccaccg catggacaag cccgccaact
     2341 gcacacacga cctgtacatg atcatgcggg agtgctggca tgccgcgccc tcccagaggc
     2401 ccaccttcaa gcagctggtg gaggacctgg accgtgtcct taccgtgacg tccaccgacg
     2461 agtacctgga cctgtcggcg cctttcgagc agtactcccc gggtggccag gacaccccca
     2521 gctccagctc ctcaggggac gactccgtgt ttgcccacga cctgctgccc ccggccccac
     2581 ccagcagtgg gggctcgcgg acgtcaactt tcttgtacaa agttggcatt ataggaaagc
     2641 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     2701 gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     2761 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2821 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2881 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2941 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     3001 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     3061 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     3121 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     3181 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     3241 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     3301 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     3361 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     3421 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     3481 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     3541 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     3601 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     3661 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     3721 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3781 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3841 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3901 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3961 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4021 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4081 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4141 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4201 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4261 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4321 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     4381 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4441 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4501 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4561 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4621 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4681 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4741 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4801 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     4861 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     4921 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     4981 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     5041 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     5101 ctactctcgc g
//
