LOCUS       sequence-125824-        5675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human FLT3 [NM_004119.2] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5675)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E091 Hs.FLT3
FEATURES             Location/Qualifiers
     source          1..5675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3166..3265)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3642..4433
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4526..5114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5015..5034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5268..5285
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5444..5471)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5563..5649)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc ggcgttggcg cgcgacggcg gccagctgcc gctgctcgtt gttttttctg
      241 caatgatatt tgggactatt acaaatcaag atctgcctgt gatcaagtgt gttttaatca
      301 atcataagaa caatgattca tcagtgggga agtcatcatc atatcccatg gtatcagaat
      361 ccccggaaga cctcgggtgt gcgttgagac cccagagctc agggacagtg tacgaagctg
      421 ccgctgtgga agtggatgta tctgcttcca tcacactgca agtgctggtc gatgccccag
      481 ggaacatttc ctgtctctgg gtctttaagc acagctccct gaattgccag ccacattttg
      541 atttacaaaa cagaggagtt gtttccatgg tcattttgaa aatgacagaa acccaagctg
      601 gagaatacct actttttatt cagagtgaag ctaccaatta cacaatattg tttacagtga
      661 gtataagaaa taccctgctt tacacattaa gaagacctta ctttagaaaa atggaaaacc
      721 aggacgccct ggtctgcata tctgagagcg ttccagagcc gatcgtggaa tgggtgcttt
      781 gcgattcaca gggggaaagc tgtaaagaag aaagtccagc tgttgttaaa aaggaggaaa
      841 aagtgcttca tgaattattt gggatggaca taaggtgctg tgccagaaat gaactgggca
      901 gggaatgcac caggctgttc acaatagatc taaatcaaac tcctcagacc acattgccac
      961 aattatttct taaagtaggg gaacccttat ggataaggtg caaagctgtt catgtgaacc
     1021 atggattcgg gctcacctgg gaattagaaa acaaagcact cgaggagggc aactactttg
     1081 agatgagtac ctattcaaca aacagaacta tgatacggat tctgtttgct tttgtatcat
     1141 cagtggcaag aaacgacacc ggatactaca cttgttcctc ttcaaagcat cccagtcaat
     1201 cagctttggt taccatcgta gaaaagggat ttataaatgc taccaattca agtgaagatt
     1261 atgaaattga ccaatatgaa gagttttgtt tttctgtcag gtttaaagcc tacccacaaa
     1321 tcagatgtac gtggaccttc tctcgaaaat catttccttg tgagcaaaag ggtcttgata
     1381 acggatacag catatccaag ttttgcaatc ataagcacca gccaggagaa tatatattcc
     1441 atgcagaaaa tgatgatgcc caatttacca aaatgttcac gctgaatata agaaggaaac
     1501 ctcaagtgct cgcagaagca tcggcaagtc aggcgtcctg tttctcggat ggatacccat
     1561 taccatcttg gacctggaag aagtgttcag acaagtctcc caactgcaca gaagagatca
     1621 cagaaggagt ctggaataga aaggctaaca gaaaagtgtt tggacagtgg gtgtcgagca
     1681 gtactctaaa catgagtgaa gccataaaag ggttcctggt caagtgctgt gcatacaatt
     1741 cccttggcac atcttgtgag acgatccttt taaactctcc aggccccttc cctttcatcc
     1801 aagacaacat ctcattctat gcaacaattg gtgtttgtct cctcttcatt gtcgttttaa
     1861 ccctgctaat ttgtcacaag tacaaaaagc aatttaggta tgaaagccag ctacagatgg
     1921 tacaggtgac cggctcctca gataatgagt acttctacgt tgatttcaga gaatatgaat
     1981 atgatctcaa atgggagttt ccaagagaaa atttagagtt tgggaaggta ctaggatcag
     2041 gtgcttttgg aaaagtgatg aacgcaacag cttatggaat tagcaaaaca ggagtctcaa
     2101 tccaggttgc cgtcaaaatg ctgaaagaaa aagcagacag ctctgaaaga gaggcactca
     2161 tgtcagaact caagatgatg acccagctgg gaagccacga gaatattgtg aacctgctgg
     2221 gggcgtgcac actgtcagga ccaatttact tgatttttga atactgttgc tatggtgatc
     2281 ttctcaacta tctaagaagt aaaagagaaa aatttcacag gacttggaca gagattttca
     2341 aggaacacaa tttcagtttt taccccactt tccaatcaca tccaaattcc agcatgcctg
     2401 gttcaagaga agttcagata cacccggact cggatcaaat ctcagggctt catgggaatt
     2461 catttcactc tgaagatgaa attgaatatg aaaaccaaaa aaggctggaa gaagaggagg
     2521 acttgaatgt gcttacattt gaagatcttc tttgctttgc atatcaagtt gccaaaggaa
     2581 tggaatttct ggaatttaag tcgtgtgttc acagagacct ggccgccagg aacgtgcttg
     2641 tcacccacgg gaaagtggtg aagatatgtg actttggatt ggctcgagat atcatgagtg
     2701 attccaacta tgttgtcagg ggcaatgccc gtctgcctgt aaaatggatg gcccccgaaa
     2761 gcctgtttga aggcatctac accattaaga gtgatgtctg gtcatatgga atattactgt
     2821 gggaaatctt ctcacttggt gtgaatcctt accctggcat tccggttgat gctaacttct
     2881 acaaactgat tcaaaatgga tttaaaatgg atcagccatt ttatgctaca gaagaaatat
     2941 acattataat gcaatcctgc tgggcttttg actcaaggaa acggccatcc ttccctaatt
     3001 tgacttcgtt tttaggatgt cagctggcag atgcagaaga agcgatgtat cagaatgtgg
     3061 atggccgtgt ttcggaatgt cctcacacct accaaaacag gcgacctttc agcagagaga
     3121 tggatttggg gctactctct ccgcaggctc aggtcgaaga ttcgtaatca actttcttgt
     3181 acaaagttgg cattatagga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     3241 tcagtcaaaa taaaatcatt atttgcatgc aagcttagat cctctagacc agccaggaca
     3301 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     3361 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     3421 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     3481 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     3541 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     3601 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     3661 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     3721 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     3781 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     3841 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     3901 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     3961 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     4021 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     4081 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     4141 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     4201 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     4261 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     4321 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     4381 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     4441 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     4501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     4921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5281 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5341 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     5401 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     5461 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     5521 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     5581 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     5641 ttttatttga tgcctggcag ttccctactc tcgcg
//
