LOCUS       sequence-125832-        3347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human GRB2 [NM_002086.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3347)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E099 Hs.GRB2
FEATURES             Location/Qualifiers
     source          1..3347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(838..937)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1314..2105
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2198..2786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2687..2706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2940..2957
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3116..3143)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3235..3321)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga agccatcgcc aaatatgact tcaaagctac tgcagacgac gagctgagct
      241 tcaaaagggg ggacatcctc aaggttttga acgaagaatg tgatcagaac tggtacaagg
      301 cagagcttaa tggaaaagac ggcttcattc ccaagaacta catagaaatg aaaccacatc
      361 cgtggttttt tggcaaaatc cccagagcca aggcagaaga aatgcttagc aaacagcggc
      421 acgatggggc ctttcttatc cgagagagtg agagcgctcc tggggacttc tccctctctg
      481 tcaagtttgg aaacgatgtg cagcacttca aggtgctccg agatggagcc gggaagtact
      541 tcctctgggt ggtgaagttc aattctttga atgagctggt ggattatcac agatctacat
      601 ctgtctccag aaaccagcag atattcctgc gggacataga acaggtgcca cagcagccga
      661 catacgtcca ggccctcttt gactttgatc cccaggagga tggagagctg ggcttccgcc
      721 ggggagattt tatccatgtc atggataact cagaccccaa ctggtggaaa ggagcttgcc
      781 acgggcagac cggcatgttt ccccgcaatt atgtcacccc cgtgaaccgg aacgtctaat
      841 caactttctt gtacaaagtt ggcattatag gaaagcattg cttatcaatt tgttgcaacg
      901 aacaggtcac tatcagtcaa aataaaatca ttatttgcat gcaagcttag atcctctaga
      961 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     1021 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     1081 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1141 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     1201 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     1261 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     1321 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     1381 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     1441 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     1501 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     1561 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     1621 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     1681 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     1741 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     1801 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     1861 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1921 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     1981 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     2041 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     2101 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2161 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2221 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2281 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2341 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2401 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2461 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2521 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2581 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2641 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2701 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2761 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2821 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2881 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2941 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3001 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3061 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3121 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3181 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3241 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3301 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcg
//
