LOCUS       sequence-125833-        3344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human GRB2 [NM_002086.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3344)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E100 Hs.GRB2-nostop
FEATURES             Location/Qualifiers
     source          1..3344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(835..934)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1311..2102
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2195..2783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2684..2703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2937..2954
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3113..3140)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3232..3318)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga agccatcgcc aaatatgact tcaaagctac tgcagacgac gagctgagct
      241 tcaaaagggg ggacatcctc aaggttttga acgaagaatg tgatcagaac tggtacaagg
      301 cagagcttaa tggaaaagac ggcttcattc ccaagaacta catagaaatg aaaccacatc
      361 cgtggttttt tggcaaaatc cccagagcca aggcagaaga aatgcttagc aaacagcggc
      421 acgatggggc ctttcttatc cgagagagtg agagcgctcc tggggacttc tccctctctg
      481 tcaagtttgg aaacgatgtg cagcacttca aggtgctccg agatggagcc gggaagtact
      541 tcctctgggt ggtgaagttc aattctttga atgagctggt ggattatcac agatctacat
      601 ctgtctccag aaaccagcag atattcctgc gggacataga acaggtgcca cagcagccga
      661 catacgtcca ggccctcttt gactttgatc cccaggagga tggagagctg ggcttccgcc
      721 ggggagattt tatccatgtc atggataact cagaccccaa ctggtggaaa ggagcttgcc
      781 acgggcagac cggcatgttt ccccgcaatt atgtcacccc cgtgaaccgg aacgtctcaa
      841 ctttcttgta caaagttggc attataggaa agcattgctt atcaatttgt tgcaacgaac
      901 aggtcactat cagtcaaaat aaaatcatta tttgcatgca agcttagatc ctctagacca
      961 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     1021 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     1081 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1141 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     1201 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     1261 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     1321 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     1381 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     1441 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     1501 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     1561 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     1621 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     1681 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     1741 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     1801 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     1861 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1921 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1981 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2041 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     2101 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2161 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2221 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2281 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2341 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2401 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2461 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2521 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2581 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2641 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2701 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2761 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2821 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2881 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2941 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3001 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3061 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3121 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     3181 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     3241 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     3301 agcctttcgt tttatttgat gcctggcagt tccctactct cgcg
//
