LOCUS       sequence-125837-        3545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human ICMT [NM_012405.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3545)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E104 Hs.ICMT-nostop
FEATURES             Location/Qualifiers
     source          1..3545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1036..1135)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1512..2303
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2396..2984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2885..2904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3138..3155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3314..3341)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3433..3519)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc gggctgcgcg gcgcgggctc cgccgggctc tgaggcgcgt ctcagcctcg
      241 ccaccttcct gctgggcgcc tcggtgctcg cgctgccgct gctcacgcgc gccggcctgc
      301 agggccgcac cgggctggcg ctctacgtgg ccgggctcaa cgcgctgctg ctgctgctct
      361 atcggccgcc tcgctaccag atagccatcc gagcttgttt cctggggttt gtgttcggct
      421 gcggcacgct gctaagtttt agccagtctt cttggagtca ctttggctgg tacatgtgct
      481 ccctgtcatt gttccactat tctgaatact tggtgacagc agtcaataat cccaaaagtc
      541 tgtccttgga ttcctttctc ctgaatcaca gcctggagta tacagtagct gctctttctt
      601 cttggttaga gttcacactt gaaaatatct tttggccaga actgaagcag attacctggc
      661 tcagtgtcac agggctgctg atggtggtct tcggagaatg tctgaggaag gcggccatgt
      721 ttacagctgg ctccaatttc aaccacgtgg tacagaatga aaaatcagat acacatactc
      781 tggtgaccag tggagtgtac gcttggtttc ggcatccttc ttacgtcggg tggttttact
      841 ggagtattgg aactcaggtg atgctgtgta accccatctg cggcgtcagc tatgccctga
      901 cagtgtggcg attcttccgc gatcgaacag aagaagaaga aatctcacta attcactttt
      961 ttggagagga gtacctggag tataagaaga gggtgcccac gggcctgcct ttcataaagg
     1021 gggtcaaggt ggacctgtca actttcttgt acaaagttgg cattatagga aagcattgct
     1081 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgcatgc
     1141 aagcttagat cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     1201 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     1261 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     1321 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     1381 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     1441 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     1501 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     1561 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     1621 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     1681 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     1741 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     1801 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     1861 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     1921 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     1981 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     2041 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     2101 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     2161 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     2221 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     2281 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     2341 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2401 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2461 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2521 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2581 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2641 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2701 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2761 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     2821 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2881 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2941 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3001 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3061 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3121 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3181 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3241 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     3301 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     3361 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     3421 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     3481 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     3541 tcgcg
//
