LOCUS       sequence-125852-        5546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human KSR2 [XM_011538224.1] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5546)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E119 Hs.KSR2
FEATURES             Location/Qualifiers
     source          1..5546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3037..3136)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3513..4304
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4397..4985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4886..4905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5139..5156
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5315..5342)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5434..5520)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga tgaggaaaac atgacgaaaa gcgaagagca gcaacctctg agtttgcaaa
      241 aagccttgca gcagtgcgag ttggttcaga acatgataga cttgagcatc tccaacctgg
      301 aagggcttag gaccaaatgt gctacctcca acgacctcac acaaaaagaa atccggaccc
      361 tggagagcaa gctggtgaag tacttcagcc ggcagctgtc ctgcaaaaag aaggtagcct
      421 tgcaggagcg caacgcggag ctggacggct tcccccagct acggcactgg ttccgaatcg
      481 tcgatgtgcg caaggaggtc ctggaggaaa tctcccccgg ccagctgagc ctggaggacc
      541 tcttggagat gacggatgaa caggtgtgcg agactgtgga gaaatacgga gccaaccggg
      601 aggagtgtgc ccgcctcaac gcctccctct cctgcctcag gaatgtccac atgtcaggag
      661 gcaacctttc caaacaagac tggaccatcc agtggcccac cacagagacg gggaaggaga
      721 acaatcccgt gtgccccccg gagcccaccc cgtggatccg cacccatctc tcccagagcc
      781 ccagggtccc gtccaagtgc gtccagcact attgtcacac cagccccact cccggggccc
      841 ctgtgtacac ccacgtggac aggcttaccg tggacgccta cccgggcttg tgcccgcccc
      901 cgccactgga gtcgggccac cgttccctgc ccccatcgcc ccggcagcgg cacgcggtcc
      961 gcaccccgcc gcgcaccccc aacatcgtca ccaccgtgac cccgccgggc acgccgccca
     1021 tgaggaagaa gaacaagctg aagcccccgg ggaccccacc gccctcctcc cgaaaactga
     1081 tacacttgat cccgggattc accgcgctgc atcggagcaa atcccacgag ttccagctgg
     1141 ggcaccgcgt ggacgaggcc cacacgccca aagccaagaa gaagagcaaa cccttgaacc
     1201 tcaagatcca cagcagcgta ggcagctgcg agaacatccc ctctcagcag cgctccccgc
     1261 tgctgtccga gcgctccctc cgctccttct ttgtgggaca cgcacctttc ctgccttcca
     1321 cccctcctgt tcacactgag gccaacttct ctgcaaacac actgtcagtg ccacgctggt
     1381 ccccgcagat ccctcgcaga gatcttggca actccatcaa gcacaggttt tccaccaagt
     1441 actggatgtc tcagacgtgc acagtctgtg ggaaagggat gctttttggc ctcaagtgta
     1501 aaaactgcaa gttaaagtgc cacaacaaat gcaccaaaga agccccaccc tgtcatcttc
     1561 tgatcatcca ccgaggagat ccagcaaggt tagtccggac agagtccgtt ccgtgtgaca
     1621 tcaacaaccc tctacggaag ccacctcgct attcagacct gcacatcagt cagacgctcc
     1681 ccaaaaccaa caaaatcaac aaggaccaca tccctgtccc ttaccagcca gactccagca
     1741 gcaacccctc ctccacgacg tcctccacgc cctcctcgcc agcacccccc ctccctccta
     1801 gtgccacgcc gccttctccc ctacaccctt ccccacagtg cacacggcag cagaagaact
     1861 tcaacctgcc agcatcccac tactacaaat acaagcagca gttcatcttc ccagatgtgg
     1921 tgccggtgcc ggagacgccg acccgggcgc cccaggtcat cctgcatccg gtgacctcga
     1981 atccaatctt ggaaggaaat ccattacttc aaattgaagt ggagccaacg tcggagaatg
     2041 aagaggtcca tgatgaggcc gaagagtcag aggatgactt cgaggagatg aacctgtccc
     2101 tcctctcggc ccggagcttc ccacgcaagg ccagccagac cagcatcttc cttcaggagt
     2161 gggacatccc ctttgagcag ctggagatcg gcgagctcat tggaaagggc cgctttgggc
     2221 aagtgtacca cggccgctgg catggcgagg tggccatccg gctgattgac attgagaggg
     2281 acaacgagga ccagctcaag gccttcaagc gggaggtgat ggcctacagg cagacacggc
     2341 atgagaacgt ggtgcttttc atgggtgcct gcatgagccc gcctcacctg gccatcatca
     2401 ccagcctctg taagggacgg acgctctatt ccgttgtgag ggatgccaaa atcgttttgg
     2461 atgtcaacaa aaccaggcag attgctcaag aaattgtgaa gggcatgggc tacctccacg
     2521 ccaagggaat cctacacaag gacctcaagt caaagaacgt cttctatgac aacggcaaag
     2581 tggtcatcac ggactttgga ctcttcagca tttctggggt gctgcaggct ggcaggcggg
     2641 aggacaaact gcgcatccag aatggctggc tatgccacct ggcaccagag atcatccgcc
     2701 agctgtcccc cgacacagag gaggataagc tccccttctc caagcactct gacgtctttg
     2761 cccttggcac aatctggtat gaactccacg ccagggaatg gcctttcaag acccaaccag
     2821 cagaggcaat aatctggcaa atgggcacag gcatgaaacc caacctcagc cagattggca
     2881 tgggaaaaga aatctcggac attcttctct tctgctgggc ctttgaacaa gaagagagac
     2941 ctaccttcac caagctcatg gacatgctgg agaaactgcc aaagcgaaac cgtcgcctgt
     3001 ctcaccctgg acatttctgg aagtctgcag agctgtaatc aactttcttg tacaaagttg
     3061 gcattatagg aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     3121 ataaaatcat tatttgcatg caagcttaga tcctctagac cagccaggac agaaatgcct
     3181 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     3241 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     3301 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     3361 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     3421 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     3481 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     3541 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     3601 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     3661 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     3721 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     3781 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     3841 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     3901 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     3961 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     4021 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     4081 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     4141 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     4201 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     4261 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     4321 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     4381 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4441 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4501 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4561 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4621 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4681 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4741 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     4801 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4861 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4921 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4981 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5041 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5101 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5161 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5221 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     5281 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     5341 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     5401 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     5461 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     5521 atgcctggca gttccctact ctcgcg
//
