LOCUS       sequence-125853-        5543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human KSR2 [XM_011538224.1] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5543)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E120 Hs.KSR2-nostop
FEATURES             Location/Qualifiers
     source          1..5543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3034..3133)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3510..4301
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4394..4982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4883..4902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5136..5153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5312..5339)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5431..5517)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga tgaggaaaac atgacgaaaa gcgaagagca gcaacctctg agtttgcaaa
      241 aagccttgca gcagtgcgag ttggttcaga acatgataga cttgagcatc tccaacctgg
      301 aagggcttag gaccaaatgt gctacctcca acgacctcac acaaaaagaa atccggaccc
      361 tggagagcaa gctggtgaag tacttcagcc ggcagctgtc ctgcaaaaag aaggtagcct
      421 tgcaggagcg caacgcggag ctggacggct tcccccagct acggcactgg ttccgaatcg
      481 tcgatgtgcg caaggaggtc ctggaggaaa tctcccccgg ccagctgagc ctggaggacc
      541 tcttggagat gacggatgaa caggtgtgcg agactgtgga gaaatacgga gccaaccggg
      601 aggagtgtgc ccgcctcaac gcctccctct cctgcctcag gaatgtccac atgtcaggag
      661 gcaacctttc caaacaagac tggaccatcc agtggcccac cacagagacg gggaaggaga
      721 acaatcccgt gtgccccccg gagcccaccc cgtggatccg cacccatctc tcccagagcc
      781 ccagggtccc gtccaagtgc gtccagcact attgtcacac cagccccact cccggggccc
      841 ctgtgtacac ccacgtggac aggcttaccg tggacgccta cccgggcttg tgcccgcccc
      901 cgccactgga gtcgggccac cgttccctgc ccccatcgcc ccggcagcgg cacgcggtcc
      961 gcaccccgcc gcgcaccccc aacatcgtca ccaccgtgac cccgccgggc acgccgccca
     1021 tgaggaagaa gaacaagctg aagcccccgg ggaccccacc gccctcctcc cgaaaactga
     1081 tacacttgat cccgggattc accgcgctgc atcggagcaa atcccacgag ttccagctgg
     1141 ggcaccgcgt ggacgaggcc cacacgccca aagccaagaa gaagagcaaa cccttgaacc
     1201 tcaagatcca cagcagcgta ggcagctgcg agaacatccc ctctcagcag cgctccccgc
     1261 tgctgtccga gcgctccctc cgctccttct ttgtgggaca cgcacctttc ctgccttcca
     1321 cccctcctgt tcacactgag gccaacttct ctgcaaacac actgtcagtg ccacgctggt
     1381 ccccgcagat ccctcgcaga gatcttggca actccatcaa gcacaggttt tccaccaagt
     1441 actggatgtc tcagacgtgc acagtctgtg ggaaagggat gctttttggc ctcaagtgta
     1501 aaaactgcaa gttaaagtgc cacaacaaat gcaccaaaga agccccaccc tgtcatcttc
     1561 tgatcatcca ccgaggagat ccagcaaggt tagtccggac agagtccgtt ccgtgtgaca
     1621 tcaacaaccc tctacggaag ccacctcgct attcagacct gcacatcagt cagacgctcc
     1681 ccaaaaccaa caaaatcaac aaggaccaca tccctgtccc ttaccagcca gactccagca
     1741 gcaacccctc ctccacgacg tcctccacgc cctcctcgcc agcacccccc ctccctccta
     1801 gtgccacgcc gccttctccc ctacaccctt ccccacagtg cacacggcag cagaagaact
     1861 tcaacctgcc agcatcccac tactacaaat acaagcagca gttcatcttc ccagatgtgg
     1921 tgccggtgcc ggagacgccg acccgggcgc cccaggtcat cctgcatccg gtgacctcga
     1981 atccaatctt ggaaggaaat ccattacttc aaattgaagt ggagccaacg tcggagaatg
     2041 aagaggtcca tgatgaggcc gaagagtcag aggatgactt cgaggagatg aacctgtccc
     2101 tcctctcggc ccggagcttc ccacgcaagg ccagccagac cagcatcttc cttcaggagt
     2161 gggacatccc ctttgagcag ctggagatcg gcgagctcat tggaaagggc cgctttgggc
     2221 aagtgtacca cggccgctgg catggcgagg tggccatccg gctgattgac attgagaggg
     2281 acaacgagga ccagctcaag gccttcaagc gggaggtgat ggcctacagg cagacacggc
     2341 atgagaacgt ggtgcttttc atgggtgcct gcatgagccc gcctcacctg gccatcatca
     2401 ccagcctctg taagggacgg acgctctatt ccgttgtgag ggatgccaaa atcgttttgg
     2461 atgtcaacaa aaccaggcag attgctcaag aaattgtgaa gggcatgggc tacctccacg
     2521 ccaagggaat cctacacaag gacctcaagt caaagaacgt cttctatgac aacggcaaag
     2581 tggtcatcac ggactttgga ctcttcagca tttctggggt gctgcaggct ggcaggcggg
     2641 aggacaaact gcgcatccag aatggctggc tatgccacct ggcaccagag atcatccgcc
     2701 agctgtcccc cgacacagag gaggataagc tccccttctc caagcactct gacgtctttg
     2761 cccttggcac aatctggtat gaactccacg ccagggaatg gcctttcaag acccaaccag
     2821 cagaggcaat aatctggcaa atgggcacag gcatgaaacc caacctcagc cagattggca
     2881 tgggaaaaga aatctcggac attcttctct tctgctgggc ctttgaacaa gaagagagac
     2941 ctaccttcac caagctcatg gacatgctgg agaaactgcc aaagcgaaac cgtcgcctgt
     3001 ctcaccctgg acatttctgg aagtctgcag agctgtcaac tttcttgtac aaagttggca
     3061 ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     3121 aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga aatgcctcga
     3181 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3241 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     3301 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3361 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     3421 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     3481 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     3541 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     3601 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     3661 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     3721 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     3781 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     3841 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     3901 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     3961 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     4021 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4081 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4141 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4201 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     4261 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     4321 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4501 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     4801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5041 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5101 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5161 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5221 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     5281 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     5341 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     5401 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     5461 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     5521 cctggcagtt ccctactctc gcg
//
