LOCUS       sequence-125854-        3875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human MAP2K1 [NM_002755.3] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3875)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E121 Hs.MAP2K1
FEATURES             Location/Qualifiers
     source          1..3875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1366..1465)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1842..2633
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2726..3314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3215..3234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3468..3485
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3644..3671)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3763..3849)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc caagaagaag ccgacgccca tccagctgaa cccggccccc gacggctctg
      241 cagttaacgg gaccagctct gcggagacca acttggaggc cttgcagaag aagctggagg
      301 agctagagct tgatgagcag cagcgaaagc gccttgaggc ctttcttacc cagaagcaga
      361 aggtgggaga actgaaggat gacgactttg agaagatcag tgagctgggg gctggcaatg
      421 gcggtgtggt gttcaaggtc tcccacaagc cttctggcct ggtcatggcc agaaagctaa
      481 ttcatctgga gatcaaaccc gcaatccgga accagatcat aagggagctg caggttctgc
      541 atgagtgcaa ctctccgtac atcgtgggct tctatggtgc gttctacagc gatggcgaga
      601 tcagtatctg catggagcac atggatggag gttctctgga tcaagtcctg aagaaagctg
      661 gaagaattcc tgaacaaatt ttaggaaaag ttagcattgc tgtaataaaa ggcctgacat
      721 atctgaggga gaagcacaag atcatgcaca gagatgtcaa gccctccaac atcctagtca
      781 actcccgtgg ggagatcaag ctctgtgact ttggggtcag cgggcagctc atcgactcca
      841 tggccaactc cttcgtgggc acaaggtcct acatgtcgcc agaaagactc caggggactc
      901 attactctgt gcagtcagac atctggagca tgggactgtc tctggtagag atggcggttg
      961 ggaggtatcc catccctcct ccagatgcca aggagctgga gctgatgttt gggtgccagg
     1021 tggaaggaga tgcggctgag accccaccca ggccaaggac ccccgggagg ccccttagct
     1081 catacggaat ggacagccga cctcccatgg caatttttga gttgttggat tacatagtca
     1141 acgagcctcc tccaaaactg cccagtggag tgttcagtct ggaatttcaa gattttgtga
     1201 ataaatgctt aataaaaaac cccgcagaga gagcagattt gaagcaactc atggttcatg
     1261 cttttatcaa gagatctgat gctgaggaag tggattttgc aggttggctc tgctccacca
     1321 tcggccttaa ccagcccagc acaccaaccc atgctgctgg cgtctaatca actttcttgt
     1381 acaaagttgg cattatagga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     1441 tcagtcaaaa taaaatcatt atttgcatgc aagcttagat cctctagacc agccaggaca
     1501 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     1561 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     1621 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1681 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     1741 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1801 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     1861 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     1921 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     1981 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2041 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2101 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2161 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2221 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2281 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2341 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2401 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2461 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2521 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2581 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     2641 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     3121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3421 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3481 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3541 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3601 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3661 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3721 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     3781 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     3841 ttttatttga tgcctggcag ttccctactc tcgcg
//
