LOCUS       sequence-125859-        3773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human MAPK1 [NM_002745.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3773)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E126 Hs.MAPK1-nostop
FEATURES             Location/Qualifiers
     source          1..3773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      184..193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1230..1265
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     protein_bind    complement(1264..1363)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1740..2531
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2624..3212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3113..3132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3366..3383
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3542..3569)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3661..3747)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc ggcggcggcg gcggcgggcg cgggcccgga gatggtccgc gggcaggtgt
      241 tcgacgtggg gccgcgctac accaacctct cgtacatcgg cgagggcgcc tacggcatgg
      301 tgtgctctgc ttatgataat gtcaacaaag ttcgagtagc tatcaagaaa atcagcccct
      361 ttgagcacca gacctactgc cagagaaccc tgagggagat aaaaatctta ctgcgcttca
      421 gacatgagaa catcattgga atcaatgaca ttattcgagc accaaccatc gagcaaatga
      481 aagatgtata tatagtacag gacctcatgg aaacagatct ttacaagctc ttgaagacac
      541 aacacctcag caatgaccat atctgctatt ttctctacca gatcctcaga gggttaaaat
      601 atatccattc agctaacgtt ctgcaccgtg acctcaagcc ttccaacctg ctgctcaaca
      661 ccacctgtga tctcaagatc tgtgactttg gcctggcccg tgttgcagat ccagaccatg
      721 atcacacagg gttcctgaca gaatatgtgg ccacacgttg gtacagggct ccagaaatta
      781 tgttgaattc caagggctac accaagtcca ttgatatttg gtctgtaggc tgcattctgg
      841 cagaaatgct ttctaacagg cccatctttc cagggaagca ttatcttgac cagctgaacc
      901 acattttggg tattcttgga tccccatcac aagaagacct gaattgtata ataaatttaa
      961 aagctaggaa ctatttgctt tctcttccac acaaaaataa ggtgccatgg aacaggctgt
     1021 tcccaaatgc tgactccaaa gctctggact tattggacaa aatgttgaca ttcaacccac
     1081 acaagaggat tgaagtagaa caggctctgg cccacccata tctggagcag tattacgacc
     1141 cgagtgacga gcccatcgcc gaagcaccat tcaagttcga catggaattg gatgacttgc
     1201 ctaaggaaaa gctcaaagaa ctaatttttg aagagactgc tagattccag ccaggataca
     1261 gatcttcaac tttcttgtac aaagttggca ttataggaaa gcattgctta tcaatttgtt
     1321 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgcatgcaa gcttagatcc
     1381 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     1441 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     1501 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     1561 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     1621 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     1681 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     1741 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     1801 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     1861 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     1921 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     1981 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     2041 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     2101 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     2161 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     2221 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     2281 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     2341 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     2401 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     2461 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     2521 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     2581 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2641 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2701 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2761 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2821 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2881 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2941 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3001 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3061 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3121 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3181 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3241 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3301 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3361 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3421 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3481 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     3541 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     3601 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     3661 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     3721 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcg
//
