LOCUS       sequence-125862-        4187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human MDM2 [NM_002392.5] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4187)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E129 Hs.MDM2
FEATURES             Location/Qualifiers
     source          1..4187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1678..1777)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2154..2945
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3038..3626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3527..3546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3780..3797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3956..3983)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4075..4161)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggt gaggagcagg caaatgtgca ataccaacat gtctgtacct actgatggtg
      241 ctgtaaccac ctcacagatt ccagcttcgg aacaagagac cctggttaga ccaaagccat
      301 tgcttttgaa gttattaaag tctgttggtg cacaaaaaga cacttatact atgaaagagg
      361 ttctttttta tcttggccag tatattatga ctaaacgatt atatgatgag aagcaacaac
      421 atattgtata ttgttcaaat gatcttctag gagatttgtt tggcgtgcca agcttctctg
      481 tgaaagagca caggaaaata tataccatga tctacaggaa cttggtagta gtcaatcagc
      541 aggaatcatc ggactcaggt acatctgtga gtgagaacag gtgtcacctt gaaggtggga
      601 gtgatcaaaa ggaccttgta caagagcttc aggaagagaa accttcatct tcacatttgg
      661 tttctagacc atctacctca tctagaagga gagcaattag tgagacagaa gaaaattcag
      721 atgaattatc tggtgaacga caaagaaaac gccacaaatc tgatagtatt tccctttcct
      781 ttgatgaaag cctggctctg tgtgtaataa gggagatatg ttgtgaaaga agcagtagca
      841 gtgaatctac agggacgcca tcgaatccgg atcttgatgc tggtgtaagt gaacattcag
      901 gtgattggtt ggatcaggat tcagtttcag atcagtttag tgtagaattt gaagttgaat
      961 ctctcgactc agaagattat agccttagtg aagaaggaca agaactctca gatgaagatg
     1021 atgaggtata tcaagttact gtgtatcagg caggggagag tgatacagat tcatttgaag
     1081 aagatcctga aatttcctta gctgactatt ggaaatgcac ttcatgcaat gaaatgaatc
     1141 ccccccttcc atcacattgc aacagatgtt gggcccttcg tgagaattgg cttcctgaag
     1201 ataaagggaa agataaaggg gaaatctctg agaaagccaa actggaaaac tcaacacaag
     1261 ctgaagaggg ctttgatgtt cctgattgta aaaaaactat agtgaatgat tccagagagt
     1321 catgtgttga agaaaatgat gataaaatta cacaagcttc acaatcacaa gaaagtgaag
     1381 actattctca gccatcaact tctagtagca ttatttatag cagccaagaa gatgtgaaag
     1441 agtttgaaag ggaagaaacc caagacaaag aagagagtgt ggaatctagt ttgcccctta
     1501 atgccattga accttgtgtg atttgtcaag gtcgacctaa aaatggttgc attgtccatg
     1561 gcaaaacagg acatcttatg gcctgcttta catgtgcaaa gaagctaaag aaaaggaata
     1621 agccctgccc agtatgtaga caaccaattc aaatgattgt gctaacttat ttcccctaat
     1681 caactttctt gtacaaagtt ggcattatag gaaagcattg cttatcaatt tgttgcaacg
     1741 aacaggtcac tatcagtcaa aataaaatca ttatttgcat gcaagcttag atcctctaga
     1801 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     1861 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     1921 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1981 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2041 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2101 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2161 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2221 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2281 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2341 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2401 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2461 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2521 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2581 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2641 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2701 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     2761 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     2821 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     2881 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     2941 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3001 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3061 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3181 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3421 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3781 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3841 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3901 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3961 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4021 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4081 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4141 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcg
//
