LOCUS       sequence-125863-        4184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human MDM2 [NM_002392.5] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4184)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E130 Hs.MDM2-nostop
FEATURES             Location/Qualifiers
     source          1..4184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1675..1774)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2151..2942
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3035..3623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3524..3543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3777..3794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3953..3980)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4072..4158)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggt gaggagcagg caaatgtgca ataccaacat gtctgtacct actgatggtg
      241 ctgtaaccac ctcacagatt ccagcttcgg aacaagagac cctggttaga ccaaagccat
      301 tgcttttgaa gttattaaag tctgttggtg cacaaaaaga cacttatact atgaaagagg
      361 ttctttttta tcttggccag tatattatga ctaaacgatt atatgatgag aagcaacaac
      421 atattgtata ttgttcaaat gatcttctag gagatttgtt tggcgtgcca agcttctctg
      481 tgaaagagca caggaaaata tataccatga tctacaggaa cttggtagta gtcaatcagc
      541 aggaatcatc ggactcaggt acatctgtga gtgagaacag gtgtcacctt gaaggtggga
      601 gtgatcaaaa ggaccttgta caagagcttc aggaagagaa accttcatct tcacatttgg
      661 tttctagacc atctacctca tctagaagga gagcaattag tgagacagaa gaaaattcag
      721 atgaattatc tggtgaacga caaagaaaac gccacaaatc tgatagtatt tccctttcct
      781 ttgatgaaag cctggctctg tgtgtaataa gggagatatg ttgtgaaaga agcagtagca
      841 gtgaatctac agggacgcca tcgaatccgg atcttgatgc tggtgtaagt gaacattcag
      901 gtgattggtt ggatcaggat tcagtttcag atcagtttag tgtagaattt gaagttgaat
      961 ctctcgactc agaagattat agccttagtg aagaaggaca agaactctca gatgaagatg
     1021 atgaggtata tcaagttact gtgtatcagg caggggagag tgatacagat tcatttgaag
     1081 aagatcctga aatttcctta gctgactatt ggaaatgcac ttcatgcaat gaaatgaatc
     1141 ccccccttcc atcacattgc aacagatgtt gggcccttcg tgagaattgg cttcctgaag
     1201 ataaagggaa agataaaggg gaaatctctg agaaagccaa actggaaaac tcaacacaag
     1261 ctgaagaggg ctttgatgtt cctgattgta aaaaaactat agtgaatgat tccagagagt
     1321 catgtgttga agaaaatgat gataaaatta cacaagcttc acaatcacaa gaaagtgaag
     1381 actattctca gccatcaact tctagtagca ttatttatag cagccaagaa gatgtgaaag
     1441 agtttgaaag ggaagaaacc caagacaaag aagagagtgt ggaatctagt ttgcccctta
     1501 atgccattga accttgtgtg atttgtcaag gtcgacctaa aaatggttgc attgtccatg
     1561 gcaaaacagg acatcttatg gcctgcttta catgtgcaaa gaagctaaag aaaaggaata
     1621 agccctgccc agtatgtaga caaccaattc aaatgattgt gctaacttat ttcccctcaa
     1681 ctttcttgta caaagttggc attataggaa agcattgctt atcaatttgt tgcaacgaac
     1741 aggtcactat cagtcaaaat aaaatcatta tttgcatgca agcttagatc ctctagacca
     1801 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     1861 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     1921 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1981 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     2041 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2101 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     2161 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2221 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2281 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2341 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2401 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2461 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2521 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2581 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2641 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2701 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2761 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     2821 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2881 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     2941 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     3001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3181 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3421 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3901 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3961 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     4021 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     4081 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4141 agcctttcgt tttatttgat gcctggcagt tccctactct cgcg
//
