LOCUS       sequence-125867-        3671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human MLST8 [NM_022372.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3671)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E134 Hs.MLST8-nostop
FEATURES             Location/Qualifiers
     source          1..3671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1162..1261)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1638..2429
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2522..3110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3011..3030
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3264..3281
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3440..3467)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3559..3645)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgaa cacctcccca ggcacggtgg gcagtgaccc ggtcatcctg gccactgcag
      241 gctacgacca caccgtgcgc ttctggcagg cccacagcgg catctgcacc cggacggtgc
      301 agcaccagga ctcccaggtg aatgccttgg aggtcacacc ggaccgcagc atgattgctg
      361 ctgcaggtta ccagcacatc cgcatgtatg atctcaactc caataaccct aaccccatca
      421 tcagctacga cggcgtcaac aagaacatcg cgtctgtggg cttccacgaa gacggccgct
      481 ggatgtacac gggcggcgag gactgcacag ccaggatctg ggacctcagg tcccggaacc
      541 tgcagtgcca gcggatcttc caggtgaacg cacccattaa ctgcgtgtgc ctgcacccca
      601 accaggcaga gctcatcgtg ggtgaccaga gcggggctat ccacatctgg gacttgaaaa
      661 cagaccacaa cgagcagctg atccctgagc ccgaggtctc catcacgtcc gcccacatcg
      721 atcccgacgc cagctacatg gcagctgtca atagcaccgg aaactgctat gtctggaatc
      781 tgacgggggg cattggtgac gaggtgaccc agctcatccc caagactaag atccctgccc
      841 acacgcgcta cgccctgcag tgtcgcttca gccccgactc cacgctcctc gccacctgct
      901 cggctgatca gacgtgcaag atctggagga cgtccaactt ctccctgatg acggagctga
      961 gcatcaagag cggcaacccc ggggagtcct cccgcggctg gatgtggggc tgcgccttct
     1021 cgggggactc ccagtacatc gtcactgctt cctcggacaa cctggcccgg ctctggtgtg
     1081 tggagactgg agagatcaag agagagtatg gcggccacca gaaggctgtt gtctgcctgg
     1141 ccttcaatga cagtgtgctg ggctcaactt tcttgtacaa agttggcatt ataggaaagc
     1201 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1261 gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     1321 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     1381 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     1441 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1501 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1561 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1621 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1681 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1741 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     1801 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     1861 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     1921 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     1981 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2041 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2101 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2161 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2221 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2281 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2341 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     2401 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     2461 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2521 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2581 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2641 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2701 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2761 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2821 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2881 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     2941 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3001 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3061 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3121 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3181 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3241 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3301 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3361 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3421 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3481 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3541 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3601 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     3661 ctactctcgc g
//
