LOCUS       sequence-125874-        4511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human NFE2L2 [NM_006164.4] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4511)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E141 Hs.NFE2L2
FEATURES             Location/Qualifiers
     source          1..4511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2002..2101)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2478..3269
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3362..3950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3851..3870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4104..4121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4280..4307)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4399..4485)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgat ggacttggag ctgccgccgc cgggactccc gtcccagcag gacatggatt
      241 tgattgacat actttggagg caagatatag atcttggagt aagtcgagaa gtatttgact
      301 tcagtcagcg acggaaagag tatgagctgg aaaaacagaa aaaacttgaa aaggaaagac
      361 aagaacaact ccaaaaggag caagagaaag cctttttcgc tcagttacaa ctagatgaag
      421 agacaggtga atttctccca attcagccag cccagcacat ccagtcagaa accagtggat
      481 ctgccaacta ctcccaggtt gcccacattc ccaaatcaga tgctttgtac tttgatgact
      541 gcatgcagct tttggcgcag acattcccgt ttgtagatga caatgaggtt tcttcggcta
      601 cgtttcagtc acttgttcct gatattcccg gtcacatcga gagcccagtc ttcattgcta
      661 ctaatcaggc tcagtcacct gaaacttctg ttgctcaggt agcccctgtt gatttagacg
      721 gtatgcaaca ggacattgag caagtttggg aggagctatt atccattcct gagttacagt
      781 gtcttaatat tgaaaatgac aagctggttg agactaccat ggttccaagt ccagaagcca
      841 aactgacaga agttgacaat tatcattttt actcatctat accctcaatg gaaaaagaag
      901 taggtaactg tagtccacat tttcttaatg cttttgagga ttccttcagc agcatcctct
      961 ccacagaaga ccccaaccag ttgacagtga actcattaaa ttcagatgcc acagtcaaca
     1021 cagattttgg tgatgaattt tattctgctt tcatagctga gcccagtatc agcaacagca
     1081 tgccctcacc tgctacttta agccattcac tctctgaact tctaaatggg cccattgatg
     1141 tttctgatct atcactttgc aaagctttca accaaaacca ccctgaaagc acagcagaat
     1201 tcaatgattc tgactccggc atttcactaa acacaagtcc cagtgtggca tcaccagaac
     1261 actcagtgga atcttccagc tatggagaca cactacttgg cctcagtgat tctgaagtgg
     1321 aagagctaga tagtgcccct ggaagtgtca aacagaatgg tcctaaaaca ccagtacatt
     1381 cttctgggga tatggtacaa cccttgtcac catctcaggg gcagagcact cacgtgcatg
     1441 atgcccaatg tgagaacaca ccagagaaag aattgcctgt aagtcctggt catcggaaaa
     1501 ccccattcac aaaagacaaa cattcaagcc gcttggaggc tcatctcaca agagatgaac
     1561 ttagggcaaa agctctccat atcccattcc ctgtagaaaa aatcattaac ctccctgttg
     1621 ttgacttcaa cgaaatgatg tccaaagagc agttcaatga agctcaactt gcattaattc
     1681 gggatatacg taggaggggt aagaataaag tggctgctca gaattgcaga aaaagaaaac
     1741 tggaaaatat agtagaacta gagcaagatt tagatcattt gaaagatgaa aaagaaaaat
     1801 tgctcaaaga aaaaggagaa aatgacaaaa gccttcacct actgaaaaaa caactcagca
     1861 ccttatatct cgaagttttc agcatgctac gtgatgaaga tggaaaacct tattctccta
     1921 gtgaatactc cctgcagcaa acaagagatg gcaatgtttt ccttgttccc aaaagtaaga
     1981 agccagatgt taagaaaaac taatcaactt tcttgtacaa agttggcatt ataggaaagc
     2041 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     2101 gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     2161 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2221 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2281 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2341 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2401 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2461 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2521 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2581 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2641 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2701 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2761 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2821 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2881 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2941 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     3001 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     3061 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     3121 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3181 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3241 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3301 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3361 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3421 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3481 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3541 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3601 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3661 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3721 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     3781 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3841 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3901 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3961 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4021 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4081 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4141 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4201 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     4261 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     4321 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     4381 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4441 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     4501 ctactctcgc g
//
