LOCUS       sequence-125875-        4508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human NFE2L2 [NM_006164.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4508)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E142 Hs.NFE2L2-nostop
FEATURES             Location/Qualifiers
     source          1..4508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1999..2098)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2475..3266
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3359..3947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3848..3867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4101..4118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4277..4304)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4396..4482)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgat ggacttggag ctgccgccgc cgggactccc gtcccagcag gacatggatt
      241 tgattgacat actttggagg caagatatag atcttggagt aagtcgagaa gtatttgact
      301 tcagtcagcg acggaaagag tatgagctgg aaaaacagaa aaaacttgaa aaggaaagac
      361 aagaacaact ccaaaaggag caagagaaag cctttttcgc tcagttacaa ctagatgaag
      421 agacaggtga atttctccca attcagccag cccagcacat ccagtcagaa accagtggat
      481 ctgccaacta ctcccaggtt gcccacattc ccaaatcaga tgctttgtac tttgatgact
      541 gcatgcagct tttggcgcag acattcccgt ttgtagatga caatgaggtt tcttcggcta
      601 cgtttcagtc acttgttcct gatattcccg gtcacatcga gagcccagtc ttcattgcta
      661 ctaatcaggc tcagtcacct gaaacttctg ttgctcaggt agcccctgtt gatttagacg
      721 gtatgcaaca ggacattgag caagtttggg aggagctatt atccattcct gagttacagt
      781 gtcttaatat tgaaaatgac aagctggttg agactaccat ggttccaagt ccagaagcca
      841 aactgacaga agttgacaat tatcattttt actcatctat accctcaatg gaaaaagaag
      901 taggtaactg tagtccacat tttcttaatg cttttgagga ttccttcagc agcatcctct
      961 ccacagaaga ccccaaccag ttgacagtga actcattaaa ttcagatgcc acagtcaaca
     1021 cagattttgg tgatgaattt tattctgctt tcatagctga gcccagtatc agcaacagca
     1081 tgccctcacc tgctacttta agccattcac tctctgaact tctaaatggg cccattgatg
     1141 tttctgatct atcactttgc aaagctttca accaaaacca ccctgaaagc acagcagaat
     1201 tcaatgattc tgactccggc atttcactaa acacaagtcc cagtgtggca tcaccagaac
     1261 actcagtgga atcttccagc tatggagaca cactacttgg cctcagtgat tctgaagtgg
     1321 aagagctaga tagtgcccct ggaagtgtca aacagaatgg tcctaaaaca ccagtacatt
     1381 cttctgggga tatggtacaa cccttgtcac catctcaggg gcagagcact cacgtgcatg
     1441 atgcccaatg tgagaacaca ccagagaaag aattgcctgt aagtcctggt catcggaaaa
     1501 ccccattcac aaaagacaaa cattcaagcc gcttggaggc tcatctcaca agagatgaac
     1561 ttagggcaaa agctctccat atcccattcc ctgtagaaaa aatcattaac ctccctgttg
     1621 ttgacttcaa cgaaatgatg tccaaagagc agttcaatga agctcaactt gcattaattc
     1681 gggatatacg taggaggggt aagaataaag tggctgctca gaattgcaga aaaagaaaac
     1741 tggaaaatat agtagaacta gagcaagatt tagatcattt gaaagatgaa aaagaaaaat
     1801 tgctcaaaga aaaaggagaa aatgacaaaa gccttcacct actgaaaaaa caactcagca
     1861 ccttatatct cgaagttttc agcatgctac gtgatgaaga tggaaaacct tattctccta
     1921 gtgaatactc cctgcagcaa acaagagatg gcaatgtttt ccttgttccc aaaagtaaga
     1981 agccagatgt taagaaaaac tcaactttct tgtacaaagt tggcattata ggaaagcatt
     2041 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca
     2101 tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     2161 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2221 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2281 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2341 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2401 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2461 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2521 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2581 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2641 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2701 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2761 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2821 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2881 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2941 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     3001 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3061 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3121 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3181 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3241 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3301 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3361 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3421 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3481 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3541 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3601 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3661 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3721 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     3781 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3841 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3901 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3961 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4021 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4081 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4141 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4201 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4261 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4321 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4381 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4441 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4501 ctctcgcg
//
