LOCUS       sequence-125876-        5603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human NFKB1 [NM_003998.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5603)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E143 Hs.NFKB1
FEATURES             Location/Qualifiers
     source          1..5603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3094..3193)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3570..4361
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4454..5042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4943..4962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5196..5213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5372..5399)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5491..5577)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc agaagatgat ccatatttgg gaaggcctga acaaatgttt catttggatc
      241 cttctttgac tcatacaata tttaatccag aagtatttca accacagatg gcactgccaa
      301 cagcagatgg cccatacctt caaatattag agcaacctaa acagagagga tttcgtttcc
      361 gttatgtatg tgaaggccca tcccatggtg gactacctgg tgcctctagt gaaaagaaca
      421 agaagtctta ccctcaggtc aaaatctgca actatgtggg accagcaaag gttattgttc
      481 agttggtcac aaatggaaaa aatatccacc tgcatgccca cagcctggtg ggaaaacact
      541 gtgaggatgg gatctgcact gtaactgctg gacccaagga catggtggtc ggcttcgcaa
      601 acctgggtat acttcatgtg acaaagaaaa aagtatttga aacactggaa gcacgaatga
      661 cagaggcgtg tataaggggc tataatcctg gactcttggt gcaccctgac cttgcctatt
      721 tgcaagcaga aggtggaggg gaccggcagc tgggagatcg ggaaaaagag ctaatccgcc
      781 aagcagctct gcagcagacc aaggagatgg acctcagcgt ggtgcggctc atgtttacag
      841 cttttcttcc ggatagcact ggcagcttca caaggcgcct ggaacccgtg gtatcagacg
      901 ccatctatga cagtaaagcc cccaatgcat ccaacttgaa aattgtaaga atggacagga
      961 cagctggatg tgtgactgga ggggaggaaa tttatcttct ttgtgacaaa gttcagaaag
     1021 atgacatcca gattcgattt tatgaagagg aagaaaatgg tggagtctgg gaaggatttg
     1081 gagatttttc ccccacagat gttcatagac aatttgccat tgtcttcaaa actccaaagt
     1141 ataaagatat taatattaca aaaccagcct ctgtgtttgt ccagcttcgg aggaaatctg
     1201 acttggaaac tagtgaacca aaacctttcc tctactatcc tgaaatcaaa gataaagaag
     1261 aagtgcagag gaaacgtcag aagctcatgc ccaatttttc ggatagtttc ggcggtggta
     1321 gtggtgctgg agctggaggc ggaggcatgt ttggtagtgg cggtggagga gggggcactg
     1381 gaagtacagg tccagggtat agcttcccac actatggatt tcctacttat ggtgggatta
     1441 ctttccatcc tggaactact aaatctaatg ctgggatgaa gcatggaacc atggacactg
     1501 aatctaaaaa ggaccctgaa ggttgtgaca aaagtgatga caaaaacact gtaaacctct
     1561 ttgggaaagt tattgaaacc acagagcaag atcaggagcc cagcgaggcc accgttggga
     1621 atggtgaggt cactctaacg tatgcaacag gaacaaaaga agagagtgct ggagttcagg
     1681 ataacctctt tctagagaag gctatgcagc ttgcaaagag gcatgccaat gcccttttcg
     1741 actacgcggt gacaggagac gtgaagatgc tgctggccgt ccagcgccat ctcactgctg
     1801 tgcaggatga gaatggggac agtgtcttac acttagcaat catccacctt cattctcaac
     1861 ttgtgaggga tctactagaa gtcacatctg gtttgatttc tgatgacatt atcaacatga
     1921 gaaatgatct gtaccagacg cccttgcact tggcagtgat cactaagcag gaagatgtgg
     1981 tggaggattt gctgagggct ggggccgacc tgagccttct ggaccgcttg ggtaactctg
     2041 ttttgcacct agctgccaaa gaaggacatg ataaagttct cagtatctta ctcaagcaca
     2101 aaaaggcagc actacttctt gaccacccca acggggacgg tctgaatgcc attcatctag
     2161 ccatgatgag caatagcctg ccatgtttgc tgctgctggt ggccgctggg gctgacgtca
     2221 atgctcagga gcagaagtcc gggcgcacag cactgcacct ggctgtggag cacgacaaca
     2281 tctcattggc aggctgcctg ctcctggagg gtgatgccca tgtggacagt actacctacg
     2341 atggaaccac acccctgcat atagcagctg ggagagggtc caccaggctg gcagctcttc
     2401 tcaaagcagc aggagcagat cccctggtgg agaactttga gcctctctat gacctggatg
     2461 actcttggga aaatgcagga gaggatgaag gagttgtgcc tggaaccacg cctctagata
     2521 tggccaccag ctggcaggta tttgacatat taaatgggaa accatatgag ccagagttta
     2581 catctgatga tttactagca caaggagaca tgaaacagct ggctgaagat gtgaagctgc
     2641 agctgtataa gttactagaa attcctgatc cagacaaaaa ctgggctact ctggcgcaga
     2701 aattaggtct ggggatactt aataatgcct tccggctgag tcctgctcct tccaaaacac
     2761 ttatggacaa ctatgaggtc tctgggggta cagtcagaga gctggtggag gccctgagac
     2821 aaatgggcta caccgaagca attgaagtga tccaggcagc ctccagccca gtgaagacca
     2881 cctctcaggc ccactcgctg cctctctcgc ctgcctccac aaggcagcaa atagacgagc
     2941 tccgagacag tgacagtgtc tgcgacagcg gcgtggagac atccttccgc aaactcagct
     3001 ttaccgagtc tctgaccagt ggtgcctcac tgctaactct caacaaaatg ccccatgatt
     3061 atgggcagga aggacctcta gaaggcaaaa tttaatcaac tttcttgtac aaagttggca
     3121 ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     3181 aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga aatgcctcga
     3241 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3301 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     3361 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3421 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     3481 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     3541 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     3601 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     3661 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     3721 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     3781 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     3841 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     3901 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     3961 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     4021 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     4081 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4141 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4201 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4261 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     4321 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     4381 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4561 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     4861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5101 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5161 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5221 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5281 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     5341 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     5401 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     5461 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     5521 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     5581 cctggcagtt ccctactctc gcg
//
