LOCUS       sequence-125877-        5600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human NFKB1 [NM_003998.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5600)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E144 Hs.NFKB1-nostop
FEATURES             Location/Qualifiers
     source          1..5600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3091..3190)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3567..4358
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4451..5039
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4940..4959
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5193..5210
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5369..5396)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5488..5574)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc agaagatgat ccatatttgg gaaggcctga acaaatgttt catttggatc
      241 cttctttgac tcatacaata tttaatccag aagtatttca accacagatg gcactgccaa
      301 cagcagatgg cccatacctt caaatattag agcaacctaa acagagagga tttcgtttcc
      361 gttatgtatg tgaaggccca tcccatggtg gactacctgg tgcctctagt gaaaagaaca
      421 agaagtctta ccctcaggtc aaaatctgca actatgtggg accagcaaag gttattgttc
      481 agttggtcac aaatggaaaa aatatccacc tgcatgccca cagcctggtg ggaaaacact
      541 gtgaggatgg gatctgcact gtaactgctg gacccaagga catggtggtc ggcttcgcaa
      601 acctgggtat acttcatgtg acaaagaaaa aagtatttga aacactggaa gcacgaatga
      661 cagaggcgtg tataaggggc tataatcctg gactcttggt gcaccctgac cttgcctatt
      721 tgcaagcaga aggtggaggg gaccggcagc tgggagatcg ggaaaaagag ctaatccgcc
      781 aagcagctct gcagcagacc aaggagatgg acctcagcgt ggtgcggctc atgtttacag
      841 cttttcttcc ggatagcact ggcagcttca caaggcgcct ggaacccgtg gtatcagacg
      901 ccatctatga cagtaaagcc cccaatgcat ccaacttgaa aattgtaaga atggacagga
      961 cagctggatg tgtgactgga ggggaggaaa tttatcttct ttgtgacaaa gttcagaaag
     1021 atgacatcca gattcgattt tatgaagagg aagaaaatgg tggagtctgg gaaggatttg
     1081 gagatttttc ccccacagat gttcatagac aatttgccat tgtcttcaaa actccaaagt
     1141 ataaagatat taatattaca aaaccagcct ctgtgtttgt ccagcttcgg aggaaatctg
     1201 acttggaaac tagtgaacca aaacctttcc tctactatcc tgaaatcaaa gataaagaag
     1261 aagtgcagag gaaacgtcag aagctcatgc ccaatttttc ggatagtttc ggcggtggta
     1321 gtggtgctgg agctggaggc ggaggcatgt ttggtagtgg cggtggagga gggggcactg
     1381 gaagtacagg tccagggtat agcttcccac actatggatt tcctacttat ggtgggatta
     1441 ctttccatcc tggaactact aaatctaatg ctgggatgaa gcatggaacc atggacactg
     1501 aatctaaaaa ggaccctgaa ggttgtgaca aaagtgatga caaaaacact gtaaacctct
     1561 ttgggaaagt tattgaaacc acagagcaag atcaggagcc cagcgaggcc accgttggga
     1621 atggtgaggt cactctaacg tatgcaacag gaacaaaaga agagagtgct ggagttcagg
     1681 ataacctctt tctagagaag gctatgcagc ttgcaaagag gcatgccaat gcccttttcg
     1741 actacgcggt gacaggagac gtgaagatgc tgctggccgt ccagcgccat ctcactgctg
     1801 tgcaggatga gaatggggac agtgtcttac acttagcaat catccacctt cattctcaac
     1861 ttgtgaggga tctactagaa gtcacatctg gtttgatttc tgatgacatt atcaacatga
     1921 gaaatgatct gtaccagacg cccttgcact tggcagtgat cactaagcag gaagatgtgg
     1981 tggaggattt gctgagggct ggggccgacc tgagccttct ggaccgcttg ggtaactctg
     2041 ttttgcacct agctgccaaa gaaggacatg ataaagttct cagtatctta ctcaagcaca
     2101 aaaaggcagc actacttctt gaccacccca acggggacgg tctgaatgcc attcatctag
     2161 ccatgatgag caatagcctg ccatgtttgc tgctgctggt ggccgctggg gctgacgtca
     2221 atgctcagga gcagaagtcc gggcgcacag cactgcacct ggctgtggag cacgacaaca
     2281 tctcattggc aggctgcctg ctcctggagg gtgatgccca tgtggacagt actacctacg
     2341 atggaaccac acccctgcat atagcagctg ggagagggtc caccaggctg gcagctcttc
     2401 tcaaagcagc aggagcagat cccctggtgg agaactttga gcctctctat gacctggatg
     2461 actcttggga aaatgcagga gaggatgaag gagttgtgcc tggaaccacg cctctagata
     2521 tggccaccag ctggcaggta tttgacatat taaatgggaa accatatgag ccagagttta
     2581 catctgatga tttactagca caaggagaca tgaaacagct ggctgaagat gtgaagctgc
     2641 agctgtataa gttactagaa attcctgatc cagacaaaaa ctgggctact ctggcgcaga
     2701 aattaggtct ggggatactt aataatgcct tccggctgag tcctgctcct tccaaaacac
     2761 ttatggacaa ctatgaggtc tctgggggta cagtcagaga gctggtggag gccctgagac
     2821 aaatgggcta caccgaagca attgaagtga tccaggcagc ctccagccca gtgaagacca
     2881 cctctcaggc ccactcgctg cctctctcgc ctgcctccac aaggcagcaa atagacgagc
     2941 tccgagacag tgacagtgtc tgcgacagcg gcgtggagac atccttccgc aaactcagct
     3001 ttaccgagtc tctgaccagt ggtgcctcac tgctaactct caacaaaatg ccccatgatt
     3061 atgggcagga aggacctcta gaaggcaaaa tttcaacttt cttgtacaaa gttggcatta
     3121 taggaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     3181 tcattatttg catgcaagct tagatcctct agaccagcca ggacagaaat gcctcgactt
     3241 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     3301 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     3361 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     3421 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     3481 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     3541 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     3601 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     3661 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     3721 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     3781 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     3841 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     3901 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     3961 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     4021 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     4081 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     4141 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     4201 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     4261 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     4321 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     4381 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     4441 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4501 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4561 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4621 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4681 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4741 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4801 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     4861 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4921 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4981 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5041 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5101 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5161 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5221 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5281 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     5341 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     5401 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     5461 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     5521 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     5581 ggcagttccc tactctcgcg
//
