LOCUS       sequence-125880-        4331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PAK1 [NM_002576.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4331)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E147 Hs.PAK1
FEATURES             Location/Qualifiers
     source          1..4331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(718..735)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(1822..1921)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2298..3089
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3182..3770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3671..3690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3924..3941
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4100..4127)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4219..4305)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtc aaataacggc ctagacattc aagacaaacc cccagcccct ccgatgagaa
      241 ataccagcac tatgattgga gccggcagca aagatgctgg aaccctaaac catggttcta
      301 aacctctgcc tccaaaccca gaggagaaga aaaagaagga ccgattttac cgatccattt
      361 tacctggaga taaaacaaat aaaaagaaag agaaagagcg gccagagatt tctctccctt
      421 cagattttga acacacaatt catgtcggtt ttgatgctgt cacaggggag tttacgggaa
      481 tgccagagca gtgggcccgc ttgcttcaga catcaaatat cactaagtcg gagcagaaga
      541 aaaacccgca ggctgttctg gatgtgttgg agttttacaa ctcgaagaag acatccaaca
      601 gccagaaata catgagcttt acagataagt cagctgagga ttacaattct tctaatgcct
      661 tgaatgtgaa ggctgtgtct gagactcctg cagtgccacc agtttcagaa gatgaggatg
      721 atgatgatga tgatgctacc ccaccaccag tgattgctcc acgcccagag cacacaaaat
      781 ctgtatacac acggtctgtg attgaaccac ttcctgtcac tccaactcgg gacgtggcta
      841 catctcccat ttcacctact gaaaataaca ccactccacc agatgctttg acccggaata
      901 ctgagaagca gaagaagaag cctaaaatgt ctgatgagga gatcttggag aaattacgaa
      961 gcatagtgag tgtgggcgat cctaagaaga aatatacacg gtttgagaag attggacaag
     1021 gtgcttcagg caccgtgtac acagcaatgg atgtggccac aggacaggag gtggccatta
     1081 agcagatgaa tcttcagcag cagcccaaga aagagctgat tattaatgag atcctggtca
     1141 tgagggaaaa caagaaccca aacattgtga attacttgga cagttacctc gtgggagatg
     1201 agctgtgggt tgttatggaa tacttggctg gaggctcctt gacagatgtg gtgacagaaa
     1261 cttgcatgga tgaaggccaa attgcagctg tgtgccgtga gtgtctgcag gctctggagt
     1321 tcttgcattc gaaccaggtc attcacagag acatcaagag tgacaatatt ctgttgggaa
     1381 tggatggctc tgtcaagcta actgactttg gattctgtgc acagataacc ccagagcaga
     1441 gcaaacggag caccatggta ggaaccccat actggatggc accagaggtt gtgacacgaa
     1501 aggcctatgg gcccaaggtt gacatctggt ccctgggcat catggccatc gaaatgattg
     1561 aaggggagcc tccatacctc aatgaaaacc ctctgagagc cttgtacctc attgccacca
     1621 atgggacccc agaacttcag aacccagaga agctgtcagc tatcttccgg gactttctga
     1681 accgctgtct cgagatggat gtggagaaga gaggttcagc taaagagctg ctacagcatc
     1741 aattcctgaa gattgccaag cccctctcca gcctcactcc actgattgct gcagctaagg
     1801 aggcaacaaa gaacaatcac taatcaactt tcttgtacaa agttggcatt ataggaaagc
     1861 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1921 gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     1981 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2041 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2101 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2161 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2221 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2281 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2341 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2401 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2461 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2521 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2581 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2641 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2701 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2761 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2821 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2881 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2941 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3001 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3061 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3121 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3181 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3241 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3301 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3361 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3421 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3481 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3541 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     3601 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3661 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3721 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3781 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3841 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3901 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3961 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4021 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     4081 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     4141 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     4201 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4261 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     4321 ctactctcgc g
//
