LOCUS       sequence-125881-        4328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PAK1 [NM_002576.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4328)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E148 Hs.PAK1-nostop
FEATURES             Location/Qualifiers
     source          1..4328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(718..735)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(1819..1918)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2295..3086
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3179..3767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3668..3687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3921..3938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4097..4124)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4216..4302)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtc aaataacggc ctagacattc aagacaaacc cccagcccct ccgatgagaa
      241 ataccagcac tatgattgga gccggcagca aagatgctgg aaccctaaac catggttcta
      301 aacctctgcc tccaaaccca gaggagaaga aaaagaagga ccgattttac cgatccattt
      361 tacctggaga taaaacaaat aaaaagaaag agaaagagcg gccagagatt tctctccctt
      421 cagattttga acacacaatt catgtcggtt ttgatgctgt cacaggggag tttacgggaa
      481 tgccagagca gtgggcccgc ttgcttcaga catcaaatat cactaagtcg gagcagaaga
      541 aaaacccgca ggctgttctg gatgtgttgg agttttacaa ctcgaagaag acatccaaca
      601 gccagaaata catgagcttt acagataagt cagctgagga ttacaattct tctaatgcct
      661 tgaatgtgaa ggctgtgtct gagactcctg cagtgccacc agtttcagaa gatgaggatg
      721 atgatgatga tgatgctacc ccaccaccag tgattgctcc acgcccagag cacacaaaat
      781 ctgtatacac acggtctgtg attgaaccac ttcctgtcac tccaactcgg gacgtggcta
      841 catctcccat ttcacctact gaaaataaca ccactccacc agatgctttg acccggaata
      901 ctgagaagca gaagaagaag cctaaaatgt ctgatgagga gatcttggag aaattacgaa
      961 gcatagtgag tgtgggcgat cctaagaaga aatatacacg gtttgagaag attggacaag
     1021 gtgcttcagg caccgtgtac acagcaatgg atgtggccac aggacaggag gtggccatta
     1081 agcagatgaa tcttcagcag cagcccaaga aagagctgat tattaatgag atcctggtca
     1141 tgagggaaaa caagaaccca aacattgtga attacttgga cagttacctc gtgggagatg
     1201 agctgtgggt tgttatggaa tacttggctg gaggctcctt gacagatgtg gtgacagaaa
     1261 cttgcatgga tgaaggccaa attgcagctg tgtgccgtga gtgtctgcag gctctggagt
     1321 tcttgcattc gaaccaggtc attcacagag acatcaagag tgacaatatt ctgttgggaa
     1381 tggatggctc tgtcaagcta actgactttg gattctgtgc acagataacc ccagagcaga
     1441 gcaaacggag caccatggta ggaaccccat actggatggc accagaggtt gtgacacgaa
     1501 aggcctatgg gcccaaggtt gacatctggt ccctgggcat catggccatc gaaatgattg
     1561 aaggggagcc tccatacctc aatgaaaacc ctctgagagc cttgtacctc attgccacca
     1621 atgggacccc agaacttcag aacccagaga agctgtcagc tatcttccgg gactttctga
     1681 accgctgtct cgagatggat gtggagaaga gaggttcagc taaagagctg ctacagcatc
     1741 aattcctgaa gattgccaag cccctctcca gcctcactcc actgattgct gcagctaagg
     1801 aggcaacaaa gaacaatcac tcaactttct tgtacaaagt tggcattata ggaaagcatt
     1861 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca
     1921 tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1981 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2041 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2101 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2161 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2221 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2281 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2341 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2401 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2461 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2521 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2581 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2641 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2701 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2761 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2821 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2881 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2941 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3001 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3061 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3121 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3301 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3541 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     3601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3781 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3961 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4021 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4081 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4141 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4201 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4261 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4321 ctctcgcg
//
