LOCUS       sequence-125883-        4265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PAK2 [NM_002577.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4265)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E150 Hs.PAK2-nostop
FEATURES             Location/Qualifiers
     source          1..4265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1756..1855)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2232..3023
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3116..3704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3605..3624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3858..3875
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4034..4061)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4153..4239)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtc tgataacgga gaactggaag ataagcctcc agcacctcct gtgcgaatga
      241 gcagcaccat ctttagcact ggaggcaaag accctttgtc agccaatcac agtttgaaac
      301 ctttgccctc tgttccagaa gagaaaaagc ccaggcataa aatcatctcc atattctcag
      361 gcacagagaa aggaagtaaa aagaaggaaa aggaacggcc agaaatttct cctccatctg
      421 attttgagca caccatccat gttggttttg atgctgttac tggagaattc actggcatgc
      481 cagaacagtg ggctcgatta ctacagacct ccaatatcac caaactagag caaaagaaga
      541 accctcaggc tgtgctggat gtcctaaagt tctacgactc caacacagtg aagcagaaat
      601 atctgagctt tactcctcct gagaaagatg gctttccttc tggaacacca gcactgaatg
      661 ccaagggaac agaagcaccc gcagtagtga cagaggagga ggatgatgat gaagagactg
      721 ctcctcccgt tattgccccg cgaccggatc atacgaaatc aatttacaca cggtctgtaa
      781 ttgaccctgt tcctgcacca gttggtgatt cacatgttga tggtgctgcc aagtctttag
      841 acaaacagaa aaagaagact aagatgacag atgaagagat tatggagaaa ttaagaacta
      901 tcgtgagcat aggtgaccct aagaaaaaat atacaagata tgaaaaaatt ggacaagggg
      961 cttctggtac agttttcact gctactgacg ttgcactggg acaggaggtt gctatcaaac
     1021 aaattaattt acagaaacag ccaaagaagg aactgatcat taacgagatt ctggtgatga
     1081 aagaattgaa aaatcccaac atcgttaact ttttggacag ttacctggta ggagatgaat
     1141 tgtttgtggt catggaatac cttgctgggg ggtcactcac tgatgtggta acagaaacgt
     1201 gcatggatga agcacagatt gctgctgtat gcagagagtg tttacaggca ttggagtttt
     1261 tacatgctaa tcaagtgatc cacagagaca tcaaaagtga caatgtactt ttgggaatgg
     1321 aaggatctgt taagctcact gactttggtt tctgtgccca gatcacccct gagcagagca
     1381 aacgcagtac catggtcgga acgccatact ggatggcacc agaggtggtt acacggaaag
     1441 cttatggccc taaagtcgac atatggtctc tgggtatcat ggctattgag atggtagaag
     1501 gagagcctcc atacctcaat gaaaatccct tgagggcctt gtacctaata gcaactaatg
     1561 gaaccccaga acttcagaat ccagagaaac tttccccaat atttcgggat ttcttaaatc
     1621 gatgtttgga aatggatgtg gaaaaaaggg gttcagccaa agaattatta cagcatcctt
     1681 tcctgaaact ggccaaaccg ttatctagct tgacaccact gatcatggca gctaaagaag
     1741 caatgaagag taaccgttca actttcttgt acaaagttgg cattatagga aagcattgct
     1801 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgcatgc
     1861 aagcttagat cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     1921 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     1981 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2041 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2101 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2161 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2221 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     2281 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2341 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     2401 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2461 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2521 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     2581 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     2641 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     2701 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     2761 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     2821 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     2881 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     2941 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3001 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3061 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3121 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3181 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3241 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3301 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3361 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3421 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3481 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     3541 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3601 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3661 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3721 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3781 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3841 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3901 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3961 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     4021 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     4081 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     4141 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4201 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     4261 tcgcg
//
