LOCUS       sequence-125885-        4325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PAK3 [NM_002578.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4325)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E152 Hs.PAK3-nostop
FEATURES             Location/Qualifiers
     source          1..4325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1816..1915)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2292..3083
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3176..3764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3665..3684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3918..3935
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4094..4121)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4213..4299)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtc tgacggtctg gataatgaag agaaaccccc ggctcctcca ctgaggatga
      241 atagtaacaa ccgggattct tcagcactca accacagctc caaaccactt cccatggccc
      301 ctgaagagaa gaataagaaa gccaggcttc gctctatctt cccaggagga ggggataaaa
      361 ccaataagaa gaaggagaaa gagcgcccag agatctctct tccttcagac tttgagcata
      421 cgattcatgt ggggtttgat gcagtcaccg gggaattcac tggaattcca gagcaatggg
      481 cacgattact ccaaacttcc aacataacaa aattggaaca gaagaagaac ccacaagctg
      541 ttctagatgt tctcaaattc tatgattcca aagaaacagt caacaaccag aaatacatga
      601 gctttacatc aggagataaa agtgcacatg gatacatagc agcccatcct tcgagtacaa
      661 aaacagcatc tgagcctcca ttggcccctc ctgtgtctga agaagaagat gaagaggaag
      721 aagaagaaga agatgaaaat gagccaccac cagttatcgc accaagacca gagcatacaa
      781 aatcaatcta tactcgttct gtggttgaat ccattgcttc accagcagta ccaaataaag
      841 aggtcacacc accctctgct gaaaatgcca attccagtac tttgtacagg aacacagatc
      901 ggcaaagaaa aaaatccaag atgacagatg aggagatctt agagaagcta agaagcattg
      961 tgagtgttgg ggacccaaag aaaaaataca caagatttga aaaaattggt caaggggcat
     1021 caggtactgt ttatacagca ctagacattg caacaggaca agaggtggcc ataaagcaga
     1081 tgaaccttca acagcaaccc aagaaggaat taattattaa tgaaattctg gtcatgaggg
     1141 aaaataagaa ccctaatatt gttaattatt tagatagcta cttggtgggt gatgaactat
     1201 gggtagtcat ggaatacttg gctggtggct ctctgactga tgtggtcaca gagacctgta
     1261 tggatgaagg acagatagca gctgtctgca gagagtgcct gcaagctttg gatttcctgc
     1321 actcaaacca ggtgatccat agagatataa agagtgacaa tattcttctc gggatggatg
     1381 gctctgttaa attgactgac tttgggttct gtgcccagat cactcctgag caaagtaaac
     1441 gaagcactat ggtgggaacc ccatattgga tggcacctga ggtggtgact cgaaaagctt
     1501 atggtccgaa agttgatatc tggtctcttg gaattatggc aattgaaatg gtggaaggtg
     1561 aaccccctta ccttaatgaa aatccactca gggcattgta tctgatagcc actaatggaa
     1621 ctccagagct ccagaatcct gagagactgt cagctgtatt ccgtgacttt ttaaatcgct
     1681 gtcttgagat ggatgtggat aggcgaggat ctgccaagga gcttttgcag catccatttt
     1741 taaaattagc caagcctctc tccagcctga ctcctctgat tatcgctgca aaggaagcaa
     1801 ttaagaacag cagccgctca actttcttgt acaaagttgg cattatagga aagcattgct
     1861 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgcatgc
     1921 aagcttagat cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     1981 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     2041 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2101 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2161 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2221 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2281 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     2341 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2401 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     2461 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2521 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2581 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     2641 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     2701 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     2761 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     2821 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     2881 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     2941 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3001 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3061 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3121 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3181 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3241 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3301 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3361 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3421 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3481 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3541 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     3601 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3661 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3721 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3781 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3841 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3901 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3961 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4021 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     4081 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     4141 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     4201 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4261 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     4321 tcgcg
//
