LOCUS       sequence-125886-        4469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PAK4 [NM_005884.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E153 Hs.PAK4
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1960..2059)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2436..3227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3320..3908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3809..3828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4062..4079
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4238..4265)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4357..4443)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtt tgggaagagg aagaagcggg tggagatctc cgcgccgtcc aacttcgagc
      241 accgcgtgca cacgggcttc gaccagcacg agcagaagtt cacggggctg ccccgccagt
      301 ggcagagcct gatcgaggag tcggctcgcc ggcccaagcc cctcgtcgac cccgcctgca
      361 tcacctccat ccagcccggg gcccccaaga ccatcgtgcg gggcagcaaa ggtgccaaag
      421 atggggccct cacgctgctg ctggacgagt ttgagaacat gtcggtgaca cgctccaact
      481 ccctgcggag agacagcccg ccgccgcccg cccgtgcccg ccaggaaaat gggatgccag
      541 aggagccggc caccacggcc agagggggcc cagggaaggc aggcagccga ggccggttcg
      601 ccggtcacag cgaggcgggt ggcggcagtg gtgacaggcg acgggcgggg ccagagaaga
      661 ggcccaagtc ttccagggag ggctcagggg gtccccagga gtcctcccgg gacaaacgcc
      721 ccctctccgg gcctgatgtc ggcacccccc agcctgctgg tctggccagt ggggcgaaac
      781 tggcagctgg ccggcccttt aacacctacc cgagggctga cacggaccac ccatcccggg
      841 gtgcccaggg ggagcctcat gacgtggccc ctaacgggcc atcagcgggg ggcctggcca
      901 tcccccagtc ctcctcctcc tcctcccggc ctcccacccg agcccgaggt gcccccagcc
      961 ctggagtgct gggaccccac gcctcagagc cccagctggc ccctccagcc tgcacccccg
     1021 ccgcccctgc tgttcctggg ccccctggcc cccgctcacc acagcgggag ccacagcgag
     1081 tatcccatga gcagttccgg gctgccctgc agctggtggt ggacccaggc gacccccgct
     1141 cctacctgga caacttcatc aagattggcg agggctccac gggcatcgtg tgcatcgcca
     1201 ccgtgcgcag ctcgggcaag ctggtggccg tcaagaagat ggacctgcgc aagcagcaga
     1261 ggcgcgagct gctcttcaac gaggtggtaa tcatgaggga ctaccagcac gagaatgtgg
     1321 tggagatgta caacagctac ctggtggggg acgagctctg ggtggtcatg gagttcctgg
     1381 aaggaggcgc cctcaccgac atcgtcaccc acaccaggat gaacgaggag cagatcgcgg
     1441 ccgtgtgcct tgcagtgctg caggccctgt cggtgctcca cgcccagggc gtcatccacc
     1501 gggacatcaa gagcgactcg atcctgctga cccatgatgg cagggtgaag ctgtcagact
     1561 ttgggttctg cgcccaggtg agcaaggaag tgccccgaag gaagtcgctg gtcggcacgc
     1621 cctactggat ggccccagag ctcatctccc gccttcccta cgggccagag gtagacatct
     1681 ggtcgctggg gataatggtg attgagatgg tggacggaga gcccccctac ttcaacgagc
     1741 cacccctcaa agccatgaag atgattcggg acaacctgcc accccgactg aagaacctgc
     1801 acaaggtgtc gccatccctg aagggcttcc tggaccgcct gctggtgcga gaccctgccc
     1861 agcgggccac ggcagccgag ctgctgaagc acccattcct ggccaaggca gggccgcctg
     1921 ccagcatcgt gcccctcatg cgccagaacc gcaccagata atcaactttc ttgtacaaag
     1981 ttggcattat aggaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2041 aaaataaaat cattatttgc atgcaagctt agatcctcta gaccagccag gacagaaatg
     2101 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     2161 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     2221 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     2281 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     2341 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     2401 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     2461 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     2521 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     2581 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     2641 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2701 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2761 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2821 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2881 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2941 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     3001 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     3061 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     3121 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     3181 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     3241 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3301 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3361 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3421 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3481 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3541 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3601 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3661 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     3721 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3781 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3841 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3901 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3961 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4021 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4081 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4141 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     4201 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     4261 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     4321 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     4381 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4441 ttgatgcctg gcagttccct actctcgcg
//
