LOCUS       sequence-125887-        4466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PAK4 [NM_005884.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4466)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E154 Hs.PAK4-nostop
FEATURES             Location/Qualifiers
     source          1..4466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1957..2056)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2433..3224
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3317..3905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3806..3825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4059..4076
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4235..4262)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4354..4440)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtt tgggaagagg aagaagcggg tggagatctc cgcgccgtcc aacttcgagc
      241 accgcgtgca cacgggcttc gaccagcacg agcagaagtt cacggggctg ccccgccagt
      301 ggcagagcct gatcgaggag tcggctcgcc ggcccaagcc cctcgtcgac cccgcctgca
      361 tcacctccat ccagcccggg gcccccaaga ccatcgtgcg gggcagcaaa ggtgccaaag
      421 atggggccct cacgctgctg ctggacgagt ttgagaacat gtcggtgaca cgctccaact
      481 ccctgcggag agacagcccg ccgccgcccg cccgtgcccg ccaggaaaat gggatgccag
      541 aggagccggc caccacggcc agagggggcc cagggaaggc aggcagccga ggccggttcg
      601 ccggtcacag cgaggcgggt ggcggcagtg gtgacaggcg acgggcgggg ccagagaaga
      661 ggcccaagtc ttccagggag ggctcagggg gtccccagga gtcctcccgg gacaaacgcc
      721 ccctctccgg gcctgatgtc ggcacccccc agcctgctgg tctggccagt ggggcgaaac
      781 tggcagctgg ccggcccttt aacacctacc cgagggctga cacggaccac ccatcccggg
      841 gtgcccaggg ggagcctcat gacgtggccc ctaacgggcc atcagcgggg ggcctggcca
      901 tcccccagtc ctcctcctcc tcctcccggc ctcccacccg agcccgaggt gcccccagcc
      961 ctggagtgct gggaccccac gcctcagagc cccagctggc ccctccagcc tgcacccccg
     1021 ccgcccctgc tgttcctggg ccccctggcc cccgctcacc acagcgggag ccacagcgag
     1081 tatcccatga gcagttccgg gctgccctgc agctggtggt ggacccaggc gacccccgct
     1141 cctacctgga caacttcatc aagattggcg agggctccac gggcatcgtg tgcatcgcca
     1201 ccgtgcgcag ctcgggcaag ctggtggccg tcaagaagat ggacctgcgc aagcagcaga
     1261 ggcgcgagct gctcttcaac gaggtggtaa tcatgaggga ctaccagcac gagaatgtgg
     1321 tggagatgta caacagctac ctggtggggg acgagctctg ggtggtcatg gagttcctgg
     1381 aaggaggcgc cctcaccgac atcgtcaccc acaccaggat gaacgaggag cagatcgcgg
     1441 ccgtgtgcct tgcagtgctg caggccctgt cggtgctcca cgcccagggc gtcatccacc
     1501 gggacatcaa gagcgactcg atcctgctga cccatgatgg cagggtgaag ctgtcagact
     1561 ttgggttctg cgcccaggtg agcaaggaag tgccccgaag gaagtcgctg gtcggcacgc
     1621 cctactggat ggccccagag ctcatctccc gccttcccta cgggccagag gtagacatct
     1681 ggtcgctggg gataatggtg attgagatgg tggacggaga gcccccctac ttcaacgagc
     1741 cacccctcaa agccatgaag atgattcggg acaacctgcc accccgactg aagaacctgc
     1801 acaaggtgtc gccatccctg aagggcttcc tggaccgcct gctggtgcga gaccctgccc
     1861 agcgggccac ggcagccgag ctgctgaagc acccattcct ggccaaggca gggccgcctg
     1921 ccagcatcgt gcccctcatg cgccagaacc gcaccagatc aactttcttg tacaaagttg
     1981 gcattatagg aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     2041 ataaaatcat tatttgcatg caagcttaga tcctctagac cagccaggac agaaatgcct
     2101 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     2161 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     2221 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2281 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     2341 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     2401 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     2461 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2521 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     2581 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2641 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2701 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     2761 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     2821 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     2881 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     2941 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     3001 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     3061 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     3121 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     3181 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     3241 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     3301 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3361 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3421 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3481 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3541 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3601 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3661 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     3721 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3781 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3841 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3901 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3961 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4021 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4081 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4141 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     4201 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     4261 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     4321 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     4381 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     4441 atgcctggca gttccctact ctcgcg
//
