LOCUS       sequence-125888-        5963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PDGFRA [NM_006206.4] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5963)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E155 Hs.PDGFRA
FEATURES             Location/Qualifiers
     source          1..5963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3454..3553)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3930..4721
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4814..5402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5303..5322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5556..5573
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5732..5759)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5851..5937)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggg gacttcccat ccggcgttcc tggtcttagg ctgtcttctc acagggctga
      241 gcctaatcct ctgccagctt tcattaccct ctatccttcc aaatgaaaat gaaaaggttg
      301 tgcagctgaa ttcatccttt tctctgagat gctttgggga gagtgaagtg agctggcagt
      361 accccatgtc tgaagaagag agctccgatg tggaaatcag aaatgaagaa aacaacagcg
      421 gcctttttgt gacggtcttg gaagtgagca gtgcctcggc ggcccacaca gggttgtaca
      481 cttgctatta caaccacact cagacagaag agaatgagct tgaaggcagg cacatttaca
      541 tctatgtgcc agacccagat gtagcctttg tacctctagg aatgacggat tatttagtca
      601 tcgtggagga tgatgattct gccattatac cttgtcgcac aactgatccc gagactcctg
      661 taaccttaca caacagtgag ggggtggtac ctgcctccta cgacagcaga cagggcttta
      721 atgggacctt cactgtaggg ccctatatct gtgaggccac cgtcaaagga aagaagttcc
      781 agaccatccc atttaacgtt tatgctttaa aagcaacatc agagctggat ctagaaatgg
      841 aagctcttaa aaccgtgtat aagtcagggg aaacgattgt ggtcacctgt gctgttttta
      901 acaatgaggt ggttgacctt caatggactt accctggaga agtgaaaggc aaaggcatca
      961 caatgctgga agaaatcaaa gtcccatcca tcaaattggt gtacactttg acggtccccg
     1021 aggccacggt gaaagacagt ggagattacg aatgtgctgc ccgccaggct accagggagg
     1081 tcaaagaaat gaagaaagtc actatttctg tccatgagaa agggttcatt gaaatcaaac
     1141 ccaccttcag ccagttggaa gctgtcaacc tgcatgaagt caaacatttt gttgtagagg
     1201 tgcgggccta cccacctccc aggatatcct ggctgaaaaa caatctgact ctgattgaaa
     1261 atctcactga gatcaccact gatgtggaaa agattcagga aataaggtat cgaagcaaat
     1321 taaagctgat ccgtgctaag gaagaagaca gtggccatta tactattgta gctcaaaatg
     1381 aagatgctgt gaagagctat acttttgaac tgttaactca agttccttca tccattctgg
     1441 acttggtcga tgatcaccat ggctcaactg ggggacagac ggtgaggtgc acagctgaag
     1501 gcacgccgct tcctgatatt gagtggatga tatgcaaaga tattaagaaa tgtaataatg
     1561 aaacttcctg gactattttg gccaacaatg tctcaaacat catcacggag atccactccc
     1621 gagacaggag taccgtggag ggccgtgtga ctttcgccaa agtggaggag accatcgccg
     1681 tgcgatgcct ggctaagaat ctccttggag ctgagaaccg agagctgaag ctggtggctc
     1741 ccaccctgcg ttctgaactc acggtggctg ctgcagtcct ggtgctgttg gtgattgtga
     1801 tcatctcact tattgtcctg gttgtcattt ggaaacagaa accgaggtat gaaattcgct
     1861 ggagggtcat tgaatcaatc agcccggatg gacatgaata tatttatgtg gacccgatgc
     1921 agctgcctta tgactcaaga tgggagtttc caagagatgg actagtgctt ggtcgggtct
     1981 tggggtctgg agcatttggg aaggtggttg aaggaacagc ctatggatta agccggtccc
     2041 aacctgtcat gaaagttgca gtgaagatgc taaaacccac ggccagatcc agtgaaaaac
     2101 aagctctcat gtctgaactg aagataatga ctcacctggg gccacatttg aacattgtaa
     2161 acttgctggg agcctgcacc aagtcaggcc ccatttacat catcacagag tattgcttct
     2221 atggagattt ggtcaactat ttgcataaga atagggatag cttcctgagc caccacccag
     2281 agaagccaaa gaaagagctg gatatctttg gattgaaccc tgctgatgaa agcacacgga
     2341 gctatgttat tttatctttt gaaaacaatg gtgactacat ggacatgaag caggctgata
     2401 ctacacagta tgtccccatg ctagaaagga aagaggtttc taaatattcc gacatccaga
     2461 gatcactcta tgatcgtcca gcctcatata agaagaaatc tatgttagac tcagaagtca
     2521 aaaacctcct ttcagatgat aactcagaag gccttacttt attggatttg ttgagcttca
     2581 cctatcaagt tgcccgagga atggagtttt tggcttcaaa aaattgtgtc caccgtgatc
     2641 tggctgctcg caacgtcctc ctggcacaag gaaaaattgt gaagatctgt gactttggcc
     2701 tggccagaga catcatgcat gattcgaact atgtgtcgaa aggcagtacc tttctgcccg
     2761 tgaagtggat ggctcctgag agcatctttg acaacctcta caccacactg agtgatgtct
     2821 ggtcttatgg cattctgctc tgggagatct tttcccttgg tggcacccct taccccggca
     2881 tgatggtgga ttctactttc tacaataaga tcaagagtgg gtaccggatg gccaagcctg
     2941 accacgctac cagtgaagtc tacgagatca tggtgaaatg ctggaacagt gagccggaga
     3001 agagaccctc cttttaccac ctgagtgaga ttgtggagaa tctgctgcct ggacaatata
     3061 aaaagagtta tgaaaaaatt cacctggact tcctgaagag tgaccatcct gctgtggcac
     3121 gcatgcgtgt ggactcagac aatgcataca ttggtgtcac ctacaaaaac gaggaagaca
     3181 agctgaagga ctgggagggt ggtctggatg agcagagact gagcgctgac agtggctaca
     3241 tcattcctct gcctgacatt gaccctgtcc ctgaggagga ggacctgggc aagaggaaca
     3301 gacacagctc gcagacctct gaagagagtg ccattgagac gggttccagc agttccacct
     3361 tcatcaagag agaggacgag accattgaag acatcgacat gatggacgac atcggcatag
     3421 actcttcaga cctggtggaa gacagcttcc tgtaatcaac tttcttgtac aaagttggca
     3481 ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     3541 aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga aatgcctcga
     3601 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3661 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     3721 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3781 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     3841 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     3901 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     3961 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     4021 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     4081 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     4141 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     4201 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     4261 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     4321 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     4381 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     4441 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4501 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4561 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4621 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     4681 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     4741 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4801 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4861 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4921 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4981 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5041 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5101 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5161 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     5221 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5281 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5341 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5401 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5461 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5521 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5581 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5641 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     5701 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     5761 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     5821 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     5881 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     5941 cctggcagtt ccctactctc gcg
//
