LOCUS       sequence-125889-        5960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PDGFRA [NM_006206.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5960)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E156 Hs.PDGFRA-nostop
FEATURES             Location/Qualifiers
     source          1..5960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3451..3550)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3927..4718
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4811..5399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5300..5319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5553..5570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5729..5756)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5848..5934)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggg gacttcccat ccggcgttcc tggtcttagg ctgtcttctc acagggctga
      241 gcctaatcct ctgccagctt tcattaccct ctatccttcc aaatgaaaat gaaaaggttg
      301 tgcagctgaa ttcatccttt tctctgagat gctttgggga gagtgaagtg agctggcagt
      361 accccatgtc tgaagaagag agctccgatg tggaaatcag aaatgaagaa aacaacagcg
      421 gcctttttgt gacggtcttg gaagtgagca gtgcctcggc ggcccacaca gggttgtaca
      481 cttgctatta caaccacact cagacagaag agaatgagct tgaaggcagg cacatttaca
      541 tctatgtgcc agacccagat gtagcctttg tacctctagg aatgacggat tatttagtca
      601 tcgtggagga tgatgattct gccattatac cttgtcgcac aactgatccc gagactcctg
      661 taaccttaca caacagtgag ggggtggtac ctgcctccta cgacagcaga cagggcttta
      721 atgggacctt cactgtaggg ccctatatct gtgaggccac cgtcaaagga aagaagttcc
      781 agaccatccc atttaacgtt tatgctttaa aagcaacatc agagctggat ctagaaatgg
      841 aagctcttaa aaccgtgtat aagtcagggg aaacgattgt ggtcacctgt gctgttttta
      901 acaatgaggt ggttgacctt caatggactt accctggaga agtgaaaggc aaaggcatca
      961 caatgctgga agaaatcaaa gtcccatcca tcaaattggt gtacactttg acggtccccg
     1021 aggccacggt gaaagacagt ggagattacg aatgtgctgc ccgccaggct accagggagg
     1081 tcaaagaaat gaagaaagtc actatttctg tccatgagaa agggttcatt gaaatcaaac
     1141 ccaccttcag ccagttggaa gctgtcaacc tgcatgaagt caaacatttt gttgtagagg
     1201 tgcgggccta cccacctccc aggatatcct ggctgaaaaa caatctgact ctgattgaaa
     1261 atctcactga gatcaccact gatgtggaaa agattcagga aataaggtat cgaagcaaat
     1321 taaagctgat ccgtgctaag gaagaagaca gtggccatta tactattgta gctcaaaatg
     1381 aagatgctgt gaagagctat acttttgaac tgttaactca agttccttca tccattctgg
     1441 acttggtcga tgatcaccat ggctcaactg ggggacagac ggtgaggtgc acagctgaag
     1501 gcacgccgct tcctgatatt gagtggatga tatgcaaaga tattaagaaa tgtaataatg
     1561 aaacttcctg gactattttg gccaacaatg tctcaaacat catcacggag atccactccc
     1621 gagacaggag taccgtggag ggccgtgtga ctttcgccaa agtggaggag accatcgccg
     1681 tgcgatgcct ggctaagaat ctccttggag ctgagaaccg agagctgaag ctggtggctc
     1741 ccaccctgcg ttctgaactc acggtggctg ctgcagtcct ggtgctgttg gtgattgtga
     1801 tcatctcact tattgtcctg gttgtcattt ggaaacagaa accgaggtat gaaattcgct
     1861 ggagggtcat tgaatcaatc agcccggatg gacatgaata tatttatgtg gacccgatgc
     1921 agctgcctta tgactcaaga tgggagtttc caagagatgg actagtgctt ggtcgggtct
     1981 tggggtctgg agcatttggg aaggtggttg aaggaacagc ctatggatta agccggtccc
     2041 aacctgtcat gaaagttgca gtgaagatgc taaaacccac ggccagatcc agtgaaaaac
     2101 aagctctcat gtctgaactg aagataatga ctcacctggg gccacatttg aacattgtaa
     2161 acttgctggg agcctgcacc aagtcaggcc ccatttacat catcacagag tattgcttct
     2221 atggagattt ggtcaactat ttgcataaga atagggatag cttcctgagc caccacccag
     2281 agaagccaaa gaaagagctg gatatctttg gattgaaccc tgctgatgaa agcacacgga
     2341 gctatgttat tttatctttt gaaaacaatg gtgactacat ggacatgaag caggctgata
     2401 ctacacagta tgtccccatg ctagaaagga aagaggtttc taaatattcc gacatccaga
     2461 gatcactcta tgatcgtcca gcctcatata agaagaaatc tatgttagac tcagaagtca
     2521 aaaacctcct ttcagatgat aactcagaag gccttacttt attggatttg ttgagcttca
     2581 cctatcaagt tgcccgagga atggagtttt tggcttcaaa aaattgtgtc caccgtgatc
     2641 tggctgctcg caacgtcctc ctggcacaag gaaaaattgt gaagatctgt gactttggcc
     2701 tggccagaga catcatgcat gattcgaact atgtgtcgaa aggcagtacc tttctgcccg
     2761 tgaagtggat ggctcctgag agcatctttg acaacctcta caccacactg agtgatgtct
     2821 ggtcttatgg cattctgctc tgggagatct tttcccttgg tggcacccct taccccggca
     2881 tgatggtgga ttctactttc tacaataaga tcaagagtgg gtaccggatg gccaagcctg
     2941 accacgctac cagtgaagtc tacgagatca tggtgaaatg ctggaacagt gagccggaga
     3001 agagaccctc cttttaccac ctgagtgaga ttgtggagaa tctgctgcct ggacaatata
     3061 aaaagagtta tgaaaaaatt cacctggact tcctgaagag tgaccatcct gctgtggcac
     3121 gcatgcgtgt ggactcagac aatgcataca ttggtgtcac ctacaaaaac gaggaagaca
     3181 agctgaagga ctgggagggt ggtctggatg agcagagact gagcgctgac agtggctaca
     3241 tcattcctct gcctgacatt gaccctgtcc ctgaggagga ggacctgggc aagaggaaca
     3301 gacacagctc gcagacctct gaagagagtg ccattgagac gggttccagc agttccacct
     3361 tcatcaagag agaggacgag accattgaag acatcgacat gatggacgac atcggcatag
     3421 actcttcaga cctggtggaa gacagcttcc tgtcaacttt cttgtacaaa gttggcatta
     3481 taggaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     3541 tcattatttg catgcaagct tagatcctct agaccagcca ggacagaaat gcctcgactt
     3601 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     3661 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     3721 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     3781 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     3841 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     3901 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     3961 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     4021 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     4081 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     4141 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     4201 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     4261 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     4321 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     4381 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     4441 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     4501 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     4561 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     4621 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     4681 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     4741 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     4801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4861 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4921 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4981 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5041 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5101 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5161 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     5221 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5281 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5341 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5401 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5461 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5521 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5581 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5641 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     5701 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     5761 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     5821 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     5881 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     5941 ggcagttccc tactctcgcg
//
