LOCUS       sequence-125890-        6014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PDGFRB [NM_002609.3] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6014)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E157 Hs.PDGFRB
FEATURES             Location/Qualifiers
     source          1..6014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1722..1868
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     protein_bind    complement(3505..3604)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3981..4772
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4865..5453
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5354..5373
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5607..5624
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5783..5810)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5902..5988)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcg gcttccgggt gcgatgccag ctctggccct caaaggcgag ctgctgttgc
      241 tgtctctcct gttacttctg gaaccacaga tctctcaggg cctggtcgtc acacccccgg
      301 ggccagagct tgtcctcaat gtctccagca ccttcgttct gacctgctcg ggttcagctc
      361 cggtggtgtg ggaacggatg tcccaggagc ccccacagga aatggccaag gcccaggatg
      421 gcaccttctc cagcgtgctc acactgacca acctcactgg gctagacacg ggagaatact
      481 tttgcaccca caatgactcc cgtggactgg agaccgatga gcggaaacgg ctctacatct
      541 ttgtgccaga tcccaccgtg ggcttcctcc ctaatgatgc cgaggaacta ttcatctttc
      601 tcacggaaat aactgagatc accattccat gccgagtaac agacccacag ctggtggtga
      661 cactgcacga gaagaaaggg gacgttgcac tgcctgtccc ctatgatcac caacgtggct
      721 tttctggtat ctttgaggac agaagctaca tctgcaaaac caccattggg gacagggagg
      781 tggattctga tgcctactat gtctacagac tccaggtgtc atccatcaac gtctctgtga
      841 acgcagtgca gactgtggtc cgccagggtg agaacatcac cctcatgtgc attgtgatcg
      901 ggaatgaggt ggtcaacttc gagtggacat acccccgcaa agaaagtggg cggctggtgg
      961 agccggtgac tgacttcctc ttggatatgc cttaccacat ccgctccatc ctgcacatcc
     1021 ccagtgccga gttagaagac tcggggacct acacctgcaa tgtgacggag agtgtgaatg
     1081 accatcagga tgaaaaggcc atcaacatca ccgtggttga gagcggctac gtgcggctcc
     1141 tgggagaggt gggcacacta caatttgctg agctgcatcg gagccggaca ctgcaggtag
     1201 tgttcgaggc ctacccaccg cccactgtcc tgtggttcaa agacaaccgc accctgggcg
     1261 actccagcgc tggcgaaatc gccctgtcca cgcgcaacgt gtcggagacc cggtatgtgt
     1321 cagagctgac actggttcgc gtgaaggtgg cagaggctgg ccactacacc atgcgggcct
     1381 tccatgagga tgctgaggtc cagctctcct tccagctaca gatcaatgtc cctgtccgag
     1441 tgctggagct aagtgagagc caccctgaca gtggggaaca gacagtccgc tgtcgtggcc
     1501 ggggcatgcc ccagccgaac atcatctggt ctgcctgcag agacctcaaa aggtgtccac
     1561 gtgagctgcc gcccacgctg ctggggaaca gttccgaaga ggagagccag ctggagacta
     1621 acgtgacgta ctgggaggag gagcaggagt ttgaggtggt gagcacactg cgtctgcagc
     1681 acgtggatcg gccactgtcg gtgcgctgca cgctgcgcaa cgctgtgggc caggacacgc
     1741 aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc
     1801 tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt tggcagaaga
     1861 agccacgtta cgagatccga tggaaggtga ttgagtctgt gagctctgac ggccatgagt
     1921 acatctacgt ggaccccatg cagctgccct atgactccac gtgggagctg ccgcgggacc
     1981 agcttgtgct gggacgcacc ctcggctctg gggcctttgg gcaggtggtg gaggccacgg
     2041 ctcatggcct gagccattct caggccacga tgaaagtggc cgtcaagatg cttaaatcca
     2101 cagcccgcag cagtgagaag caagccctta tgtcggagct gaagatcatg agtcaccttg
     2161 ggccccacct gaacgtggtc aacctgttgg gggcctgcac caaaggagga cccatctata
     2221 tcatcactga gtactgccgc tacggagacc tggtggacta cctgcaccgc aacaaacaca
     2281 ccttcctgca gcaccactcc gacaagcgcc gcccgcccag cgcggagctc tacagcaatg
     2341 ctctgcccgt tgggctcccc ctgcccagcc atgtgtcctt gaccggggag agcgacggtg
     2401 gctacatgga catgagcaag gacgagtcgg tggactatgt gcccatgctg gacatgaaag
     2461 gagacgtcaa atatgcagac atcgagtcct ccaactacat ggccccttac gataactacg
     2521 ttccctctgc ccctgagagg acctgccgag caactttgat caacgagtct ccagtgctaa
     2581 gctacatgga cctcgtgggc ttcagctacc aggtggccaa tggcatggag tttctggcct
     2641 ccaagaactg cgtccacaga gacctggcgg ctaggaacgt gctcatctgt gaaggcaagc
     2701 tggtcaagat ctgtgacttt ggcctggctc gagacatcat gcgggactcg aattacatct
     2761 ccaaaggcag cacctttttg cctttaaagt ggatggctcc ggagagcatc ttcaacagcc
     2821 tctacaccac cctgagcgac gtgtggtcct tcgggatcct gctctgggag atcttcacct
     2881 tgggtggcac cccttaccca gagctgccca tgaacgagca gttctacaat gccatcaaac
     2941 ggggttaccg catggcccag cctgcccatg cctccgacga gatctatgag atcatgcaga
     3001 agtgctggga agagaagttt gagattcggc cccccttctc ccagctggtg ctgcttctcg
     3061 agagactgtt gggcgaaggt tacaaaaaga agtaccagca ggtggatgag gagtttctga
     3121 ggagtgacca cccagccatc cttcggtccc aggcccgctt gcctgggttc catggcctcc
     3181 gatctcccct ggacaccagc tccgtcctct atactgccgt gcagcccaat gagggtgaca
     3241 acgactatat catccccctg cctgacccca aacccgaggt tgctgacgag ggcccactgg
     3301 agggttcccc cagcctagcc agctccaccc tgaatgaagt caacacctcc tcaaccatct
     3361 cctgtgacag ccccctggag ccccaggacg aaccagagcc agagccccag cttgagctcc
     3421 aggtggagcc ggagccagag ctggaacagt tgccggattc ggggtgccct gcgcctcggg
     3481 cggaagcaga ggatagcttc ctgtaatcaa ctttcttgta caaagttggc attataggaa
     3541 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     3601 tttgcatgca agcttagatc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     3661 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     3721 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     3781 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     3841 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     3901 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     3961 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     4021 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     4081 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     4141 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     4201 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     4261 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     4321 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     4381 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     4441 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     4501 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     4561 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     4621 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     4681 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     4741 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     4801 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4861 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4921 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4981 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5041 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5101 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5161 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5221 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     5281 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5341 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5401 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5461 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5521 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5581 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5641 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     5701 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     5761 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     5821 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     5881 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     5941 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     6001 tccctactct cgcg
//
