LOCUS       sequence-125891-        6011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PDGFRB [NM_002609.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6011)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E158 Hs.PDGFRB-nostop
FEATURES             Location/Qualifiers
     source          1..6011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1722..1868
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     protein_bind    complement(3502..3601)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3978..4769
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4862..5450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5351..5370
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5604..5621
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5780..5807)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5899..5985)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcg gcttccgggt gcgatgccag ctctggccct caaaggcgag ctgctgttgc
      241 tgtctctcct gttacttctg gaaccacaga tctctcaggg cctggtcgtc acacccccgg
      301 ggccagagct tgtcctcaat gtctccagca ccttcgttct gacctgctcg ggttcagctc
      361 cggtggtgtg ggaacggatg tcccaggagc ccccacagga aatggccaag gcccaggatg
      421 gcaccttctc cagcgtgctc acactgacca acctcactgg gctagacacg ggagaatact
      481 tttgcaccca caatgactcc cgtggactgg agaccgatga gcggaaacgg ctctacatct
      541 ttgtgccaga tcccaccgtg ggcttcctcc ctaatgatgc cgaggaacta ttcatctttc
      601 tcacggaaat aactgagatc accattccat gccgagtaac agacccacag ctggtggtga
      661 cactgcacga gaagaaaggg gacgttgcac tgcctgtccc ctatgatcac caacgtggct
      721 tttctggtat ctttgaggac agaagctaca tctgcaaaac caccattggg gacagggagg
      781 tggattctga tgcctactat gtctacagac tccaggtgtc atccatcaac gtctctgtga
      841 acgcagtgca gactgtggtc cgccagggtg agaacatcac cctcatgtgc attgtgatcg
      901 ggaatgaggt ggtcaacttc gagtggacat acccccgcaa agaaagtggg cggctggtgg
      961 agccggtgac tgacttcctc ttggatatgc cttaccacat ccgctccatc ctgcacatcc
     1021 ccagtgccga gttagaagac tcggggacct acacctgcaa tgtgacggag agtgtgaatg
     1081 accatcagga tgaaaaggcc atcaacatca ccgtggttga gagcggctac gtgcggctcc
     1141 tgggagaggt gggcacacta caatttgctg agctgcatcg gagccggaca ctgcaggtag
     1201 tgttcgaggc ctacccaccg cccactgtcc tgtggttcaa agacaaccgc accctgggcg
     1261 actccagcgc tggcgaaatc gccctgtcca cgcgcaacgt gtcggagacc cggtatgtgt
     1321 cagagctgac actggttcgc gtgaaggtgg cagaggctgg ccactacacc atgcgggcct
     1381 tccatgagga tgctgaggtc cagctctcct tccagctaca gatcaatgtc cctgtccgag
     1441 tgctggagct aagtgagagc caccctgaca gtggggaaca gacagtccgc tgtcgtggcc
     1501 ggggcatgcc ccagccgaac atcatctggt ctgcctgcag agacctcaaa aggtgtccac
     1561 gtgagctgcc gcccacgctg ctggggaaca gttccgaaga ggagagccag ctggagacta
     1621 acgtgacgta ctgggaggag gagcaggagt ttgaggtggt gagcacactg cgtctgcagc
     1681 acgtggatcg gccactgtcg gtgcgctgca cgctgcgcaa cgctgtgggc caggacacgc
     1741 aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc
     1801 tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt tggcagaaga
     1861 agccacgtta cgagatccga tggaaggtga ttgagtctgt gagctctgac ggccatgagt
     1921 acatctacgt ggaccccatg cagctgccct atgactccac gtgggagctg ccgcgggacc
     1981 agcttgtgct gggacgcacc ctcggctctg gggcctttgg gcaggtggtg gaggccacgg
     2041 ctcatggcct gagccattct caggccacga tgaaagtggc cgtcaagatg cttaaatcca
     2101 cagcccgcag cagtgagaag caagccctta tgtcggagct gaagatcatg agtcaccttg
     2161 ggccccacct gaacgtggtc aacctgttgg gggcctgcac caaaggagga cccatctata
     2221 tcatcactga gtactgccgc tacggagacc tggtggacta cctgcaccgc aacaaacaca
     2281 ccttcctgca gcaccactcc gacaagcgcc gcccgcccag cgcggagctc tacagcaatg
     2341 ctctgcccgt tgggctcccc ctgcccagcc atgtgtcctt gaccggggag agcgacggtg
     2401 gctacatgga catgagcaag gacgagtcgg tggactatgt gcccatgctg gacatgaaag
     2461 gagacgtcaa atatgcagac atcgagtcct ccaactacat ggccccttac gataactacg
     2521 ttccctctgc ccctgagagg acctgccgag caactttgat caacgagtct ccagtgctaa
     2581 gctacatgga cctcgtgggc ttcagctacc aggtggccaa tggcatggag tttctggcct
     2641 ccaagaactg cgtccacaga gacctggcgg ctaggaacgt gctcatctgt gaaggcaagc
     2701 tggtcaagat ctgtgacttt ggcctggctc gagacatcat gcgggactcg aattacatct
     2761 ccaaaggcag cacctttttg cctttaaagt ggatggctcc ggagagcatc ttcaacagcc
     2821 tctacaccac cctgagcgac gtgtggtcct tcgggatcct gctctgggag atcttcacct
     2881 tgggtggcac cccttaccca gagctgccca tgaacgagca gttctacaat gccatcaaac
     2941 ggggttaccg catggcccag cctgcccatg cctccgacga gatctatgag atcatgcaga
     3001 agtgctggga agagaagttt gagattcggc cccccttctc ccagctggtg ctgcttctcg
     3061 agagactgtt gggcgaaggt tacaaaaaga agtaccagca ggtggatgag gagtttctga
     3121 ggagtgacca cccagccatc cttcggtccc aggcccgctt gcctgggttc catggcctcc
     3181 gatctcccct ggacaccagc tccgtcctct atactgccgt gcagcccaat gagggtgaca
     3241 acgactatat catccccctg cctgacccca aacccgaggt tgctgacgag ggcccactgg
     3301 agggttcccc cagcctagcc agctccaccc tgaatgaagt caacacctcc tcaaccatct
     3361 cctgtgacag ccccctggag ccccaggacg aaccagagcc agagccccag cttgagctcc
     3421 aggtggagcc ggagccagag ctggaacagt tgccggattc ggggtgccct gcgcctcggg
     3481 cggaagcaga ggatagcttc ctgtcaactt tcttgtacaa agttggcatt ataggaaagc
     3541 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     3601 gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     3661 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     3721 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     3781 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     3841 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     3901 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     3961 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     4021 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     4081 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     4141 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     4201 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     4261 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     4321 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     4381 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     4441 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     4501 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     4561 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     4621 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     4681 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     4741 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     4801 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4861 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4921 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4981 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     5041 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5101 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5161 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5221 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     5281 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5341 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5401 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5461 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5521 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5581 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5641 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5701 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     5761 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     5821 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     5881 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     5941 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     6001 ctactctcgc g
//
