LOCUS       sequence-125892-        4364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PDPK1 [NM_002613.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4364)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E159 Hs.PDPK1
FEATURES             Location/Qualifiers
     source          1..4364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1855..1954)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2331..3122
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3215..3803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3704..3723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3957..3974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4133..4160)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4252..4338)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc caggaccacc agccagctgt atgacgccgt gcccatccag tccagcgtgg
      241 tgttatgttc ctgcccatcc ccatcaatgg tgaggaccca gactgagtcc agcacgcccc
      301 ctggcattcc tggtggcagc aggcagggcc ccgccatgga cggcactgca gccgagcctc
      361 ggcccggcgc cggctccctg cagcatgccc agcctccgcc gcagcctcgg aagaagcggc
      421 ctgaggactt caagtttggg aaaatccttg gggaaggctc tttttccacg gttgtcctgg
      481 ctcgagaact ggcaacctcc agagaatatg cgattaaaat tctggagaag cgacatatca
      541 taaaagagaa caaggtcccc tatgtaacca gagagcggga tgtcatgtcg cgcctggatc
      601 accccttctt tgttaagctt tacttcacat ttcaggacga cgagaagctg tatttcggcc
      661 ttagttatgc caaaaatgga gaactactta aatatattcg caaaatcggt tcattcgatg
      721 agacctgtac ccgattttac acggctgaga ttgtgtctgc tttagagtac ttgcacggca
      781 agggcatcat tcacagggac cttaaaccgg aaaacatttt gttaaatgaa gatatgcaca
      841 tccagatcac agattttgga acagcaaaag tcttatcccc agagagcaaa caagccaggg
      901 ccaactcatt cgtgggaaca gcgcagtacg tttctccaga gctgctcacg gagaagtccg
      961 cctgtaagag ttcagacctt tgggctcttg gatgcataat ataccagctt gtggcaggac
     1021 tcccaccatt ccgagctgga aacgagtatc ttatatttca gaagatcatt aagttggaat
     1081 atgactttcc agaaaaattc ttccctaagg caagagacct cgtggagaaa cttttggttt
     1141 tagatgccac aaagcggtta ggctgtgagg aaatggaagg atacggacct cttaaagcac
     1201 acccgttctt cgagtccgtc acgtgggaga acctgcacca gcagacgcct ccgaagctca
     1261 ccgcttacct gccggctatg tcggaagacg acgaggactg ctatggcaat tatgacaatc
     1321 tcctgagcca gtttggctgc atgcaggtgt cttcgtcctc ctcctcacac tccctgtcag
     1381 cctccgacac gggcctgccc cagaggtcag gcagcaacat agagcagtac attcacgatc
     1441 tggactcgaa ctcctttgaa ctggacttac agttttccga agatgagaag aggttgttgt
     1501 tggagaagca ggctggcgga aacccttggc accagtttgt agaaaataat ttaatactaa
     1561 agatgggccc agtggataag cggaagggtt tatttgcaag acgacgacag ctgttgctca
     1621 cagaaggacc acatttatat tatgtggatc ctgtcaacaa agttctgaaa ggtgaaattc
     1681 cttggtcaca agaacttcga ccagaggcca agaattttaa aactttcttt gtccacacgc
     1741 ctaacaggac gtattatctg atggacccca gcgggaacgc acacaagtgg tgcaggaaga
     1801 tccaggaggt ttggaggcag cgataccaga gccacccgga cgccgctgtg cagtaatcaa
     1861 ctttcttgta caaagttggc attataggaa agcattgctt atcaatttgt tgcaacgaac
     1921 aggtcactat cagtcaaaat aaaatcatta tttgcatgca agcttagatc ctctagacca
     1981 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     2041 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     2101 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     2161 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     2221 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2281 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     2341 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2401 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2461 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2521 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2581 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2641 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2701 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2761 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2821 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2881 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2941 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     3001 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     3061 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     3121 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     3181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3361 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3601 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3901 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3961 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4021 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4081 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     4141 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     4201 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     4261 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4321 agcctttcgt tttatttgat gcctggcagt tccctactct cgcg
//
