LOCUS       sequence-125893-        4361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PDPK1 [NM_002613.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4361)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E160 Hs.PDPK1-nostop
FEATURES             Location/Qualifiers
     source          1..4361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1852..1951)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2328..3119
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3212..3800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3701..3720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3954..3971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4130..4157)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4249..4335)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc caggaccacc agccagctgt atgacgccgt gcccatccag tccagcgtgg
      241 tgttatgttc ctgcccatcc ccatcaatgg tgaggaccca gactgagtcc agcacgcccc
      301 ctggcattcc tggtggcagc aggcagggcc ccgccatgga cggcactgca gccgagcctc
      361 ggcccggcgc cggctccctg cagcatgccc agcctccgcc gcagcctcgg aagaagcggc
      421 ctgaggactt caagtttggg aaaatccttg gggaaggctc tttttccacg gttgtcctgg
      481 ctcgagaact ggcaacctcc agagaatatg cgattaaaat tctggagaag cgacatatca
      541 taaaagagaa caaggtcccc tatgtaacca gagagcggga tgtcatgtcg cgcctggatc
      601 accccttctt tgttaagctt tacttcacat ttcaggacga cgagaagctg tatttcggcc
      661 ttagttatgc caaaaatgga gaactactta aatatattcg caaaatcggt tcattcgatg
      721 agacctgtac ccgattttac acggctgaga ttgtgtctgc tttagagtac ttgcacggca
      781 agggcatcat tcacagggac cttaaaccgg aaaacatttt gttaaatgaa gatatgcaca
      841 tccagatcac agattttgga acagcaaaag tcttatcccc agagagcaaa caagccaggg
      901 ccaactcatt cgtgggaaca gcgcagtacg tttctccaga gctgctcacg gagaagtccg
      961 cctgtaagag ttcagacctt tgggctcttg gatgcataat ataccagctt gtggcaggac
     1021 tcccaccatt ccgagctgga aacgagtatc ttatatttca gaagatcatt aagttggaat
     1081 atgactttcc agaaaaattc ttccctaagg caagagacct cgtggagaaa cttttggttt
     1141 tagatgccac aaagcggtta ggctgtgagg aaatggaagg atacggacct cttaaagcac
     1201 acccgttctt cgagtccgtc acgtgggaga acctgcacca gcagacgcct ccgaagctca
     1261 ccgcttacct gccggctatg tcggaagacg acgaggactg ctatggcaat tatgacaatc
     1321 tcctgagcca gtttggctgc atgcaggtgt cttcgtcctc ctcctcacac tccctgtcag
     1381 cctccgacac gggcctgccc cagaggtcag gcagcaacat agagcagtac attcacgatc
     1441 tggactcgaa ctcctttgaa ctggacttac agttttccga agatgagaag aggttgttgt
     1501 tggagaagca ggctggcgga aacccttggc accagtttgt agaaaataat ttaatactaa
     1561 agatgggccc agtggataag cggaagggtt tatttgcaag acgacgacag ctgttgctca
     1621 cagaaggacc acatttatat tatgtggatc ctgtcaacaa agttctgaaa ggtgaaattc
     1681 cttggtcaca agaacttcga ccagaggcca agaattttaa aactttcttt gtccacacgc
     1741 ctaacaggac gtattatctg atggacccca gcgggaacgc acacaagtgg tgcaggaaga
     1801 tccaggaggt ttggaggcag cgataccaga gccacccgga cgccgctgtg cagtcaactt
     1861 tcttgtacaa agttggcatt ataggaaagc attgcttatc aatttgttgc aacgaacagg
     1921 tcactatcag tcaaaataaa atcattattt gcatgcaagc ttagatcctc tagaccagcc
     1981 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     2041 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     2101 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2161 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     2221 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2281 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     2341 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2401 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2461 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2521 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2581 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2641 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2701 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2761 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2821 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2881 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2941 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     3001 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     3061 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     3121 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     3361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3601 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3901 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3961 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4021 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4081 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     4141 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     4201 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     4261 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     4321 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc g
//
