LOCUS       sequence-125896-        5900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3CA [NM_006218.2] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5900)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E163 Hs.PIK3CA
FEATURES             Location/Qualifiers
     source          1..5900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3391..3490)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3867..4658
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4751..5339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5240..5259
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5493..5510
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5669..5696)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5788..5874)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc tccacgacca tcatcaggtg aactgtgggg catccacttg atgcccccaa
      241 gaatcctagt agaatgttta ctaccaaatg gaatgatagt gactttagaa tgcctccgtg
      301 aggctacatt aataaccata aagcatgaac tatttaaaga agcaagaaaa taccccctcc
      361 atcaacttct tcaagatgaa tcttcttaca ttttcgtaag tgttactcaa gaagcagaaa
      421 gggaagaatt ttttgatgaa acaagacgac tttgtgacct tcggcttttt caaccctttt
      481 taaaagtaat tgaaccagta ggcaaccgtg aagaaaagat cctcaatcga gaaattggtt
      541 ttgctatcgg catgccagtg tgtgaatttg atatggttaa agatccagaa gtacaggact
      601 tccgaagaaa tattctgaac gtttgtaaag aagctgtgga tcttagggac ctcaattcac
      661 ctcatagtag agcaatgtat gtctatcctc caaatgtaga atcttcacca gaattgccaa
      721 agcacatata taataaatta gataaagggc aaataatagt ggtgatctgg gtaatagttt
      781 ctccaaataa tgacaagcag aagtatactc tgaaaatcaa ccatgactgt gtaccagaac
      841 aagtaattgc tgaagcaatc aggaaaaaaa ctcgaagtat gttgctatcc tctgaacaac
      901 taaaactctg tgttttagaa tatcagggca agtatatttt aaaagtgtgt ggatgtgatg
      961 aatacttcct agaaaaatat cctctgagtc agtataagta tataagaagc tgtataatgc
     1021 ttgggaggat gcccaatttg atgttgatgg ctaaagaaag cctttattct caactgccaa
     1081 tggactgttt tacaatgcca tcttattcca gacgcatttc cacagctaca ccatatatga
     1141 atggagaaac atctacaaaa tccctttggg ttataaatag tgcactcaga ataaaaattc
     1201 tttgtgcaac ctacgtgaat gtaaatattc gagacattga taagatctat gttcgaacag
     1261 gtatctacca tggaggagaa cccttatgtg acaatgtgaa cactcaaaga gtaccttgtt
     1321 ccaatcccag gtggaatgaa tggctgaatt atgatatata cattcctgat cttcctcgtg
     1381 ctgctcgact ttgcctttcc atttgctctg ttaaaggccg aaagggtgct aaagaggaac
     1441 actgtccatt ggcatgggga aatataaact tgtttgatta cacagacact ctagtatctg
     1501 gaaaaatggc tttgaatctt tggccagtac ctcatggatt agaagatttg ctgaacccta
     1561 ttggtgttac tggatcaaat ccaaataaag aaactccatg cttagagttg gagtttgact
     1621 ggttcagcag tgtggtaaag ttcccagata tgtcagtgat tgaagagcat gccaattggt
     1681 ctgtatcccg agaagcagga tttagctatt cccacgcagg actgagtaac agactagcta
     1741 gagacaatga attaagggaa aatgacaaag aacagctcaa agcaatttct acacgagatc
     1801 ctctctctga aatcactgag caggagaaag attttctatg gagtcacaga cactattgtg
     1861 taactatccc cgaaattcta cccaaattgc ttctgtctgt taaatggaat tctagagatg
     1921 aagtagccca gatgtattgc ttggtaaaag attggcctcc aatcaaacct gaacaggcta
     1981 tggaacttct ggactgtaat tacccagatc ctatggttcg aggttttgct gttcggtgct
     2041 tggaaaaata tttaacagat gacaaacttt ctcagtattt aattcagcta gtacaggtcc
     2101 taaaatatga acaatatttg gataacttgc ttgtgagatt tttactgaag aaagcattga
     2161 ctaatcaaag gattgggcac tttttctttt ggcatttaaa atctgagatg cacaataaaa
     2221 cagttagcca gaggtttggc ctgcttttgg agtcctattg tcgtgcatgt gggatgtatt
     2281 tgaagcacct gaataggcaa gtcgaggcaa tggaaaagct cattaactta actgacattc
     2341 tcaaacagga gaagaaggat gaaacacaaa aggtacagat gaagttttta gttgagcaaa
     2401 tgaggcgacc agatttcatg gatgctctac agggctttct gtctcctcta aaccctgctc
     2461 atcaactagg aaacctcagg cttgaagagt gtcgaattat gtcctctgca aaaaggccac
     2521 tgtggttgaa ttgggagaac ccagacatca tgtcagagtt actgtttcag aacaatgaga
     2581 tcatctttaa aaatggggat gatttacggc aagatatgct aacacttcaa attattcgta
     2641 ttatggaaaa tatctggcaa aatcaaggtc ttgatcttcg aatgttacct tatggttgtc
     2701 tgtcaatcgg tgactgtgtg ggacttattg aggtggtgcg aaattctcac actattatgc
     2761 aaattcagtg caaaggcggc ttgaaaggtg cactgcagtt caacagccac acactacatc
     2821 agtggctcaa agacaagaac aaaggagaaa tatatgatgc agccattgac ctgtttacac
     2881 gttcatgtgc tggatactgt gtagctacct tcattttggg aattggagat cgtcacaata
     2941 gtaacatcat ggtgaaagac gatggacaac tgtttcatat agattttgga cactttttgg
     3001 atcacaagaa gaaaaaattt ggttataaac gagaacgtgt gccatttgtt ttgacacagg
     3061 atttcttaat agtgattagt aaaggagccc aagaatgcac aaagacaaga gaatttgaga
     3121 ggtttcagga gatgtgttac aaggcttatc tagctattcg acagcatgcc aatctcttca
     3181 taaatctttt ctcaatgatg cttggctctg gaatgccaga actacaatct tttgatgaca
     3241 ttgcatacat tcgaaagacc ctagccttag ataaaactga gcaagaggct ttggagtatt
     3301 tcatgaaaca aatgaatgat gcacatcatg gtggctggac aacaaaaatg gattggatct
     3361 tccacacaat taaacagcat gcattgaact aatcaacttt cttgtacaaa gttggcatta
     3421 taggaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     3481 tcattatttg catgcaagct tagatcctct agaccagcca ggacagaaat gcctcgactt
     3541 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     3601 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     3661 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     3721 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     3781 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     3841 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     3901 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     3961 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     4021 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     4081 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     4141 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     4201 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     4261 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     4321 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     4381 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     4441 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     4501 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     4561 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     4621 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     4681 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     4741 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4801 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4861 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4921 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4981 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5041 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5101 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     5161 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5221 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5281 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5341 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5401 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5461 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5521 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5581 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     5641 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     5701 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     5761 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     5821 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     5881 ggcagttccc tactctcgcg
//
