LOCUS       sequence-125898-        5906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3CB [NM_006219.2] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5906)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E165 Hs.PIK3CB
FEATURES             Location/Qualifiers
     source          1..5906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             394..402
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(1701..1712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3397..3496)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3873..4664
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4757..5345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5246..5265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5499..5516
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5675..5702)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5794..5880)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtg cttcagtttc ataatgcctc ctgctatggc agacatcctt gacatctggg
      241 cggtggattc acagatagca tctgatggct ccatacctgt ggatttcctt ttgcccactg
      301 ggatttatat ccagttggag gtacctcggg aagctaccat ttcttatatt aagcagatgt
      361 tatggaagca agttcacaat tacccaatgt tcaacctcct tatggatatt gactcctata
      421 tgtttgcatg tgtgaatcag actgctgtat atgaggagct tgaagatgaa acacgaagac
      481 tctgtgatgt cagacctttt cttccagttc tcaaattagt gacaagaagt tgtgacccag
      541 gggaaaaatt agactcaaaa attggagtcc ttataggaaa aggtctgcat gaatttgatt
      601 ccttgaagga tcctgaagta aatgaatttc gaagaaaaat gcgcaaattc agcgaggaaa
      661 aaatcctgtc acttgtggga ttgtcttgga tggactggct aaaacaaaca tatccaccag
      721 agcatgaacc atccatccct gaaaacttag aagataaact ttatggggga aagctcatcg
      781 tagctgttca ttttgaaaac tgccaggacg tgtttagctt tcaagtgtct cctaatatga
      841 atcctatcaa agtaaatgaa ttggcaatcc aaaaacgttt gactattcat gggaaggaag
      901 atgaagttag cccctatgat tatgtgttgc aagtcagcgg gagagtagaa tatgtttttg
      961 gtgatcatcc actaattcag ttccagtata tccggaactg tgtgatgaac agagccctgc
     1021 cccattttat acttgtggaa tgctgcaaga tcaagaaaat gtatgaacaa gaaatgattg
     1081 ccatagaggc tgccataaat cgaaattcat ctaatcttcc tcttccatta ccaccaaaga
     1141 aaacacgaat tatttctcat gtttgggaaa ataacaaccc tttccaaatt gtcttggtta
     1201 agggaaataa acttaacaca gaggaaactg taaaagttca tgtcagggct ggtctttttc
     1261 atggtactga gctcctgtgt aaaaccatcg taagctcaga ggtatcaggg aaaaatgatc
     1321 atatttggaa tgaaccactg gaatttgata ttaatatttg tgacttacca agaatggctc
     1381 gattatgttt tgctgtttat gcagttttgg ataaagtaaa aacgaagaaa tcaacgaaaa
     1441 ctattaatcc ctctaaatat cagaccatca ggaaagctgg aaaagtgcat tatcctgtag
     1501 cgtgggtaaa tacgatggtt tttgacttta aaggacaatt gagaactgga gacataatat
     1561 tacacagctg gtcttcattt cctgatgaac tcgaagaaat gttgaatcca atgggaactg
     1621 ttcaaacaaa tccatatact gaaaatgcaa cagctttgca tgttaaattt ccagagaata
     1681 aaaaacaacc ttattattac cctcccttcg ataagattat tgaaaaggca gctgagattg
     1741 caagcagtga tagtgctaat gtgtcaagtc gaggtggaaa aaagtttctt cctgtattga
     1801 aagaaatctt ggacagggat cccttgtctc aactgtgtga aaatgaaatg gatcttattt
     1861 ggactttgcg acaagactgc cgagagattt tcccacaatc actgccaaaa ttactgctgt
     1921 caatcaagtg gaataaactt gaggatgttg ctcagcttca ggcgctgctt cagatttggc
     1981 ctaaactgcc cccccgggag gccctagagc ttctggattt caactatcca gaccagtacg
     2041 ttcgagaata tgctgtaggc tgcctgcgac agatgagtga tgaagaactt tctcaatatc
     2101 ttttacaact ggtgcaagtg ttaaaatatg agccttttct tgattgtgcc ctctctagat
     2161 tcctattaga aagagcactt ggtaatcgga ggatagggca gtttctattt tggcatctta
     2221 ggtcagaagt gcacattcct gctgtctcag tacaatttgg tgtcatcctt gaagcatact
     2281 gccggggaag tgtggggcac atgaaagtgc tttctaagca ggttgaagca ctcaataagt
     2341 taaaaacttt aaatagttta atcaaactga atgccgtgaa gttaaacaga gccaaaggga
     2401 aggaggccat gcatacctgt ttaaaacaga gtgcttaccg ggaagccctc tctgacctgc
     2461 agtcacccct gaacccatgt gttatcctct cagaactcta tgttgaaaag tgcaaataca
     2521 tggattccaa aatgaagcct ttgtggctgg tatacaataa caaggtattt ggtgaggatt
     2581 cagttggagt gatttttaaa aatggtgatg atttacgaca ggatatgttg acactccaaa
     2641 tgttgcgctt gatggattta ctctggaaag aagctggttt ggatcttcgg atgttgcctt
     2701 atggctgttt agcaacagga gatcgctctg gcctcattga agttgtgagc acctctgaaa
     2761 caattgctga cattcagctg aacagtagca atgtggctgc tgcagcagcc ttcaacaaag
     2821 atgcccttct gaactggctt aaagaataca actctgggga tgacctggac cgagccattg
     2881 aggaatttac actgtcctgt gctggctact gtgtagcttc ttatgtcctt gggattggtg
     2941 acagacatag tgacaacatc atggtcaaaa aaactggcca gctcttccac attgactttg
     3001 gacatattct tggaaatttc aaatctaagt ttggcattaa aagggagcga gtgcctttta
     3061 ttcttaccta tgatttcatc catgtcattc aacaaggaaa aacaggaaat acagaaaagt
     3121 ttggccggtt ccgccagtgt tgtgaggatg catatctgat tttacgacgg catgggaatc
     3181 tcttcatcac tctctttgcg ctgatgttga ctgcagggct tcctgaactc acatcagtca
     3241 aagatataca gtatcttaag gactctcttg cattagggaa gagtgaagaa gaagcactca
     3301 aacagtttaa gcaaaaattt gatgaggcgc tcagggaaag ctggactact aaagtgaact
     3361 ggatggccca cacagttcgg aaagactaca gatcttaatc aactttcttg tacaaagttg
     3421 gcattatagg aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     3481 ataaaatcat tatttgcatg caagcttaga tcctctagac cagccaggac agaaatgcct
     3541 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     3601 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     3661 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     3721 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     3781 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     3841 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     3901 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     3961 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     4021 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     4081 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     4141 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     4201 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     4261 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     4321 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     4381 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     4441 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     4501 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     4561 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     4621 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     4681 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     4741 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4801 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4861 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4921 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4981 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5041 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5101 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     5161 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5221 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5281 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5341 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5401 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5461 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5521 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5581 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     5641 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     5701 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     5761 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     5821 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     5881 atgcctggca gttccctact ctcgcg
//
