LOCUS       sequence-125901-        5825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3CD [NM_005026.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5825)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E168 Hs.PIK3CD-nostop
FEATURES             Location/Qualifiers
     source          1..5825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3316..3415)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3792..4583
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4676..5264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5165..5184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5418..5435
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5594..5621)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5713..5799)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc ccctggggtg gactgcccca tggaattctg gaccaaggag gagaatcaga
      241 gcgttgtggt tgacttcctg ctgcccacag gggtctacct gaacttccct gtgtcccgca
      301 atgccaacct cagcaccatc aagcagctgc tgtggcaccg cgcccagtat gagccgctct
      361 tccacatgct cagtggcccc gaggcctatg tgttcacctg catcaaccag acagcggagc
      421 agcaagagct ggaggacgag caacggcgtc tgtgtgacgt gcagcccttc ctgcccgtcc
      481 tgcgcctggt ggcccgtgag ggcgaccgcg tgaagaagct catcaactca cagatcagcc
      541 tcctcatcgg caaaggcctc cacgagtttg actccttgtg cgacccagaa gtgaacgact
      601 ttcgcgccaa gatgtgccaa ttctgcgagg aggcggccgc ccgccggcag cagctgggct
      661 gggaggcctg gctgcagtac agtttccccc tgcagctgga gccctcggct caaacctggg
      721 ggcctggtac cctgcggctc ccgaaccggg cccttctggt caacgttaag tttgagggca
      781 gcgaggagag cttcaccttc caggtgtcca ccaaggacgt gccgctggcg ctgatggcct
      841 gtgccctgcg gaagaaggcc acagtgttcc ggcagccgct ggtggagcag ccggaagact
      901 acacgctgca ggtgaacggc aggcatgagt acctgtatgg cagctacccg ctctgccagt
      961 tccagtacat ctgcagctgc ctgcacagtg ggttgacccc tcacctgacc atggtccatt
     1021 cctcctccat cctcgccatg cgggatgagc agagcaaccc tgccccccag gtccagaaac
     1081 cgcgtgccaa accacctccc attcctgcga agaagccttc ctctgtgtcc ctgtggtccc
     1141 tggagcagcc gttccgcatc gagctcatcc agggcagcaa agtgaacgcc gacgagcgga
     1201 tgaagctggt ggtgcaggcc gggcttttcc acggcaacga gatgctgtgc aagacggtgt
     1261 ccagctcgga ggtgagcgtg tgctcggagc ccgtgtggaa gcagcggctg gagttcgaca
     1321 tcaacatctg cgacctgccc cgcatggccc gtctctgctt tgcgctgtac gccgtgatcg
     1381 agaaagccaa gaaggctcgc tccaccaaga agaagtccaa gaaggcggac tgccccattg
     1441 cctgggccaa cctcatgctg tttgactaca aggaccagct taagaccggg gaacgctgcc
     1501 tctacatgtg gccctccgtc ccagatgaga agggcgagct gctgaacccc acgggcactg
     1561 tgcgcagtaa ccccaacacg gatagcgccg ctgccctgct catctgcctg cccgaggtgg
     1621 ccccgcaccc cgtgtactac cccgccctgg agaagatctt ggagctgggg cgacacagcg
     1681 agtgtgtgca tgtcaccgag gaggagcagc tgcagctgcg ggaaatcctg gagcggcggg
     1741 ggtctgggga gctgtatgag cacgagaagg acctggtgtg gaagctgcgg catgaagtcc
     1801 aggagcactt cccggaggcg ctagcccggc tgctgctggt caccaagtgg aacaagcatg
     1861 aggatgtggc ccagatgctc tacctgctgt gctcctggcc ggagctgccc gtcctgagcg
     1921 ccctggagct gctagacttc agcttccccg attgccacgt aggctccttc gccatcaagt
     1981 cgctgcggaa actgacggac gatgagctgt tccagtacct gctgcagctg gtgcaggtgc
     2041 tcaagtacga gtcctacctg gactgcgagc tgaccaaatt cctgctggac cgggccctgg
     2101 ccaaccgcaa gatcggccac ttccttttct ggcacctccg ctccgagatg cacgtgccgt
     2161 cggtggccct gcgcttcggc ctcatcctgg aggcctactg caggggcagc acccaccaca
     2221 tgaaggtgct gatgaagcag ggggaagcac tgagcaaact gaaggccctg aatgacttcg
     2281 tcaagctgag ctctcagaag acccccaagc cccagaccaa ggagctgatg cacttgtgca
     2341 tgcggcagga ggcctaccta gaggccctct cccacctgca gtccccactc gaccccagca
     2401 ccctgctggc tgaagtctgc gtggagcagt gcaccttcat ggactccaag atgaagcccc
     2461 tgtggatcat gtacagcaac gaggaggcag gcagcggcgg cagcgtgggc atcatcttta
     2521 agaacgggga tgacctccgg caggacatgc tgaccctgca gatgatccag ctcatggacg
     2581 tcctgtggaa gcaggagggg ctggacctga ggatgacccc ctatggctgc ctccccaccg
     2641 gggaccgcac aggcctcatt gaggtggtac tccgttcaga caccatcgcc aacatccaac
     2701 tcaacaagag caacatggca gccacagccg ccttcaacaa ggatgccctg ctcaactggc
     2761 tgaagtccaa gaacccgggg gaggccctgg atcgagccat tgaggagttc accctctcct
     2821 gtgctggcta ttgtgtggcc acatatgtgc tgggcattgg cgatcggcac agcgacaaca
     2881 tcatgatccg agagagtggg cagctgttcc acattgattt tggccacttt ctggggaatt
     2941 tcaagaccaa gtttggaatc aaccgcgagc gtgtcccatt catcctcacc tacgactttg
     3001 tccatgtgat tcagcagggg aagactaata atagtgagaa atttgaacgg ttccggggct
     3061 actgtgaaag ggcctacacc atcctgcggc gccacgggct tctcttcctc cacctctttg
     3121 ccctgatgcg ggcggcaggc ctgcctgagc tcagctgctc caaagacatc cagtatctca
     3181 aggactccct ggcactgggg aaaacagagg aggaggcact gaagcacttc cgagtgaagt
     3241 ttaacgaagc cctccgtgag agctggaaaa ccaaagtgaa ctggctggcc cacaacgtgt
     3301 ccaaagacaa caggcagtca actttcttgt acaaagttgg cattatagga aagcattgct
     3361 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgcatgc
     3421 aagcttagat cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     3481 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     3541 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     3601 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     3661 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     3721 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     3781 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     3841 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     3901 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     3961 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     4021 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     4081 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     4141 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     4201 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     4261 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     4321 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     4381 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     4441 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     4501 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     4561 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     4621 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4681 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4741 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4801 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4861 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4921 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4981 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5041 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     5101 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5161 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5221 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5281 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5341 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5401 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5461 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5521 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     5581 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     5641 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     5701 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     5761 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     5821 tcgcg
//
