LOCUS       sequence-125902-        6002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3CG [NM_002649.3] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6002)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E169 Hs.PIK3CG
FEATURES             Location/Qualifiers
     source          1..6002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3092..3103
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3493..3592)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3969..4760
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4853..5441
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5342..5361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5595..5612
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5771..5798)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5890..5976)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga gctggagaac tataaacagc ccgtggtgct gagagaggac aactgccgaa
      241 ggcgccggag gatgaagccg cgcagtgctg cggccagcct gtcctccatg gagctcatcc
      301 ccatcgagtt cgtgctgccc accagccagc gcaaatgcaa gagccccgaa acggcgctgc
      361 tgcacgtggc cggccacggc aacgtggagc agatgaaggc ccaggtgtgg ctgcgagcgc
      421 tggagaccag cgtggcggcg gacttctacc accggctggg accgcatcac ttcctcctgc
      481 tctatcagaa gaaggggcag tggtacgaga tctacgacaa gtaccaggtg gtgcagactc
      541 tggactgcct gcgctactgg aaggccacgc accggagccc gggccagatc cacctggtgc
      601 agcggcaccc gccctccgag gagtcccaag ccttccagcg gcagctcacg gcgctgattg
      661 gctatgacgt cactgacgtc agcaacgtgc acgacgatga gctggagttc acgcgccgtg
      721 gcttggtgac cccgcgcatg gcggaggtgg ccagccgcga ccccaagctc tacgccatgc
      781 acccgtgggt gacgtccaag cccctcccgg agtacctgtg gaagaagatt gccaacaact
      841 gcatcttcat cgtcattcac cgcagcacca ccagccagac cattaaggtc tcacccgacg
      901 acacccccgg cgccatcctg cagagcttct tcaccaagat ggccaagaag aaatctctga
      961 tggatattcc cgaaagccaa agcgaacagg attttgtgct gcgcgtctgt ggccgggatg
     1021 agtacctggt gggcgaaacg cccatcaaaa acttccagtg ggtgaggcac tgcctcaaga
     1081 acggagaaga gattcacgtg gtactggaca cgcctccaga cccggcccta gacgaggtga
     1141 ggaaggaaga gtggccgctg gtggacgact gcacgggagt caccggctac catgagcagc
     1201 ttaccatcca cggcaaggac cacgagagtg tgttcaccgt gtccctgtgg gactgcgacc
     1261 gcaagttcag ggtcaagatc agaggcattg atatccccgt cctgcctcgg aacaccgacc
     1321 tcacagtttt tgtagaggca aacatccagc atgggcaaca agtcctttgc caaaggagaa
     1381 ccagccccaa acccttcaca gaggaggtgc tgtggaatgt gtggcttgag ttcagtatca
     1441 aaatcaaaga cttgcccaaa ggggctctac tgaacctcca gatctactgc ggtaaagctc
     1501 cagcactgtc cagcaaggcc tctgcagagt cccccagttc tgagtccaag ggcaaagttc
     1561 agcttctcta ttatgtgaac ctgctgctga tagaccaccg tttcctcctg cgccgtggag
     1621 aatacgtcct ccacatgtgg cagatatctg ggaagggaga agaccaagga agcttcaatg
     1681 ctgacaaact cacgtctgca actaacccag acaaggagaa ctcaatgtcc atctccattc
     1741 ttctggacaa ttactgccac ccgatagccc tgcctaagca tcagcccacc cctgacccgg
     1801 aaggggaccg ggttcgagca gaaatgccca accagcttcg caagcaattg gaggcgatca
     1861 tagccactga tccacttaac cctctcacag cagaggacaa agaattgctc tggcatttta
     1921 gatacgaaag ccttaagcac ccaaaagcat atcctaagct atttagttca gtgaaatggg
     1981 gacagcaaga aattgtggcc aaaacatacc aattgttggc cagaagggaa gtctgggatc
     2041 aaagtgcttt ggatgttggg ttaacaatgc agctcctgga ctgcaacttc tcagatgaaa
     2101 atgtaagagc cattgcagtt cagaaactgg agagcttgga ggacgatgat gttctgcatt
     2161 accttctaca attggtccag gctgtgaaat ttgaaccata ccatgatagt gcccttgcca
     2221 gatttctgct gaagcgtggt ttaagaaaca aaagaattgg tcactttttg ttttggttct
     2281 tgagaagtga gatagcccag tccagacact atcagcagag gttcgctgtg attctggaag
     2341 cctatctgag gggctgtggc acagccatgc tgcacgactt tacccaacaa gtccaagtaa
     2401 tcgagatgtt acaaaaagtc acccttgata ttaaatcgct ctctgctgaa aagtatgacg
     2461 tcagttccca agttatttca caacttaaac aaaagcttga aaacctgcag aattctcaac
     2521 tccccgaaag ctttagagtt ccatatgatc ctggactgaa agcaggagcg ctggcaattg
     2581 aaaaatgtaa agtaatggcc tccaagaaaa aaccactatg gcttgagttt aaatgtgccg
     2641 atcctacagc cctatcaaat gaaacaattg gaattatctt taaacatggt gatgatctgc
     2701 gccaagacat gcttatttta cagattctac gaatcatgga gtctatttgg gagactgaat
     2761 ctttggatct atgcctcctg ccatatggtt gcatttcaac tggtgacaaa ataggaatga
     2821 tcgagattgt gaaagacgcc acgacaattg ccaaaattca gcaaagcaca gtgggcaaca
     2881 cgggagcatt taaagatgaa gtcctgaatc actggctcaa agaaaaatcc cctactgaag
     2941 aaaagtttca ggcagcagtg gagagatttg tttattcctg tgcaggctac tgtgtggcaa
     3001 cctttgttct tggaataggc gacagacaca atgacaatat tatgatcacc gagacaggaa
     3061 acctatttca tattgacttc gggcacattc ttgggaatta caaaagtttc ctgggcatta
     3121 ataaagagag agtgccattt gtgctaaccc ctgacttcct ctttgtgatg ggaacttctg
     3181 gaaagaagac aagcccacac ttccagaaat ttcaggacat ctgtgttaag gcttatctag
     3241 cccttcgtca tcacacaaac ctactgatca tcctgttctc catgatgctg atgacaggaa
     3301 tgccccagtt aacaagcaaa gaagacattg aatatatccg ggatgccctc acagtgggga
     3361 aaaatgagga ggatgctaaa aagtattttc ttgatcagat cgaagtttgc agagacaaag
     3421 gatggactgt gcagtttaat tggtttctac atcttgttct tggcatcaaa caaggagaga
     3481 aacattcagc ctaatcaact ttcttgtaca aagttggcat tataggaaag cattgcttat
     3541 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgcatgcaag
     3601 cttagatcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     3661 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     3721 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     3781 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     3841 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     3901 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     3961 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     4021 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     4081 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     4141 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     4201 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     4261 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     4321 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     4381 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     4441 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     4501 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     4561 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     4621 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     4681 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     4741 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     4801 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4861 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4921 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4981 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5041 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5101 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5161 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5221 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
     5281 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5341 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5401 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5461 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5521 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5581 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5641 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5701 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     5761 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     5821 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     5881 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     5941 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     6001 cg
//
