LOCUS       sequence-125903-        5999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3CG [NM_002649.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5999)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E170 Hs.PIK3CG-nostop
FEATURES             Location/Qualifiers
     source          1..5999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3092..3103
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3490..3589)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3966..4757
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4850..5438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5339..5358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5592..5609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5768..5795)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5887..5973)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga gctggagaac tataaacagc ccgtggtgct gagagaggac aactgccgaa
      241 ggcgccggag gatgaagccg cgcagtgctg cggccagcct gtcctccatg gagctcatcc
      301 ccatcgagtt cgtgctgccc accagccagc gcaaatgcaa gagccccgaa acggcgctgc
      361 tgcacgtggc cggccacggc aacgtggagc agatgaaggc ccaggtgtgg ctgcgagcgc
      421 tggagaccag cgtggcggcg gacttctacc accggctggg accgcatcac ttcctcctgc
      481 tctatcagaa gaaggggcag tggtacgaga tctacgacaa gtaccaggtg gtgcagactc
      541 tggactgcct gcgctactgg aaggccacgc accggagccc gggccagatc cacctggtgc
      601 agcggcaccc gccctccgag gagtcccaag ccttccagcg gcagctcacg gcgctgattg
      661 gctatgacgt cactgacgtc agcaacgtgc acgacgatga gctggagttc acgcgccgtg
      721 gcttggtgac cccgcgcatg gcggaggtgg ccagccgcga ccccaagctc tacgccatgc
      781 acccgtgggt gacgtccaag cccctcccgg agtacctgtg gaagaagatt gccaacaact
      841 gcatcttcat cgtcattcac cgcagcacca ccagccagac cattaaggtc tcacccgacg
      901 acacccccgg cgccatcctg cagagcttct tcaccaagat ggccaagaag aaatctctga
      961 tggatattcc cgaaagccaa agcgaacagg attttgtgct gcgcgtctgt ggccgggatg
     1021 agtacctggt gggcgaaacg cccatcaaaa acttccagtg ggtgaggcac tgcctcaaga
     1081 acggagaaga gattcacgtg gtactggaca cgcctccaga cccggcccta gacgaggtga
     1141 ggaaggaaga gtggccgctg gtggacgact gcacgggagt caccggctac catgagcagc
     1201 ttaccatcca cggcaaggac cacgagagtg tgttcaccgt gtccctgtgg gactgcgacc
     1261 gcaagttcag ggtcaagatc agaggcattg atatccccgt cctgcctcgg aacaccgacc
     1321 tcacagtttt tgtagaggca aacatccagc atgggcaaca agtcctttgc caaaggagaa
     1381 ccagccccaa acccttcaca gaggaggtgc tgtggaatgt gtggcttgag ttcagtatca
     1441 aaatcaaaga cttgcccaaa ggggctctac tgaacctcca gatctactgc ggtaaagctc
     1501 cagcactgtc cagcaaggcc tctgcagagt cccccagttc tgagtccaag ggcaaagttc
     1561 agcttctcta ttatgtgaac ctgctgctga tagaccaccg tttcctcctg cgccgtggag
     1621 aatacgtcct ccacatgtgg cagatatctg ggaagggaga agaccaagga agcttcaatg
     1681 ctgacaaact cacgtctgca actaacccag acaaggagaa ctcaatgtcc atctccattc
     1741 ttctggacaa ttactgccac ccgatagccc tgcctaagca tcagcccacc cctgacccgg
     1801 aaggggaccg ggttcgagca gaaatgccca accagcttcg caagcaattg gaggcgatca
     1861 tagccactga tccacttaac cctctcacag cagaggacaa agaattgctc tggcatttta
     1921 gatacgaaag ccttaagcac ccaaaagcat atcctaagct atttagttca gtgaaatggg
     1981 gacagcaaga aattgtggcc aaaacatacc aattgttggc cagaagggaa gtctgggatc
     2041 aaagtgcttt ggatgttggg ttaacaatgc agctcctgga ctgcaacttc tcagatgaaa
     2101 atgtaagagc cattgcagtt cagaaactgg agagcttgga ggacgatgat gttctgcatt
     2161 accttctaca attggtccag gctgtgaaat ttgaaccata ccatgatagt gcccttgcca
     2221 gatttctgct gaagcgtggt ttaagaaaca aaagaattgg tcactttttg ttttggttct
     2281 tgagaagtga gatagcccag tccagacact atcagcagag gttcgctgtg attctggaag
     2341 cctatctgag gggctgtggc acagccatgc tgcacgactt tacccaacaa gtccaagtaa
     2401 tcgagatgtt acaaaaagtc acccttgata ttaaatcgct ctctgctgaa aagtatgacg
     2461 tcagttccca agttatttca caacttaaac aaaagcttga aaacctgcag aattctcaac
     2521 tccccgaaag ctttagagtt ccatatgatc ctggactgaa agcaggagcg ctggcaattg
     2581 aaaaatgtaa agtaatggcc tccaagaaaa aaccactatg gcttgagttt aaatgtgccg
     2641 atcctacagc cctatcaaat gaaacaattg gaattatctt taaacatggt gatgatctgc
     2701 gccaagacat gcttatttta cagattctac gaatcatgga gtctatttgg gagactgaat
     2761 ctttggatct atgcctcctg ccatatggtt gcatttcaac tggtgacaaa ataggaatga
     2821 tcgagattgt gaaagacgcc acgacaattg ccaaaattca gcaaagcaca gtgggcaaca
     2881 cgggagcatt taaagatgaa gtcctgaatc actggctcaa agaaaaatcc cctactgaag
     2941 aaaagtttca ggcagcagtg gagagatttg tttattcctg tgcaggctac tgtgtggcaa
     3001 cctttgttct tggaataggc gacagacaca atgacaatat tatgatcacc gagacaggaa
     3061 acctatttca tattgacttc gggcacattc ttgggaatta caaaagtttc ctgggcatta
     3121 ataaagagag agtgccattt gtgctaaccc ctgacttcct ctttgtgatg ggaacttctg
     3181 gaaagaagac aagcccacac ttccagaaat ttcaggacat ctgtgttaag gcttatctag
     3241 cccttcgtca tcacacaaac ctactgatca tcctgttctc catgatgctg atgacaggaa
     3301 tgccccagtt aacaagcaaa gaagacattg aatatatccg ggatgccctc acagtgggga
     3361 aaaatgagga ggatgctaaa aagtattttc ttgatcagat cgaagtttgc agagacaaag
     3421 gatggactgt gcagtttaat tggtttctac atcttgttct tggcatcaaa caaggagaga
     3481 aacattcagc ctcaactttc ttgtacaaag ttggcattat aggaaagcat tgcttatcaa
     3541 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc atgcaagctt
     3601 agatcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     3661 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     3721 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     3781 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     3841 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     3901 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     3961 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     4021 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     4081 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     4141 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     4201 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     4261 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     4321 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     4381 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     4441 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     4501 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     4561 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     4621 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     4681 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     4741 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     4801 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4981 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5221 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5701 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     5761 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     5821 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     5881 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     5941 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcg
//
