LOCUS       sequence-125904-        4868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3R1 [NM_181523.2] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4868)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E171 Hs.PIK3R1
FEATURES             Location/Qualifiers
     source          1..4868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2359..2458)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2835..3626
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3719..4307
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4208..4227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4461..4478
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4637..4664)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4756..4842)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgag tgctgagggg taccagtaca gagcgctgta tgattataaa aaggaaagag
      241 aagaagatat tgacttgcac ttgggtgaca tattgactgt gaataaaggg tccttagtag
      301 ctcttggatt cagtgatgga caggaagcca ggcctgaaga aattggctgg ttaaatggct
      361 ataatgaaac cacaggggaa aggggggact ttccgggaac ttacgtagaa tatattggaa
      421 ggaaaaaaat ctcgcctccc acaccaaagc cccggccacc tcggcctctt cctgttgcac
      481 caggttcttc gaaaactgaa gcagatgttg aacaacaagc tttgactctc ccggatcttg
      541 cagagcagtt tgcccctcct gacattgccc cgcctcttct tatcaagctc gtggaagcca
      601 ttgaaaagaa aggtctggaa tgttcaactc tatacagaac acagagctcc agcaacctgg
      661 cagaattacg acagcttctt gattgtgata caccctccgt ggacttggaa atgatcgatg
      721 tgcacgtttt ggctgacgct ttcaaacgct atctcctgga cttaccaaat cctgtcattc
      781 cagcagccgt ttacagtgaa atgatttctt tagctccaga agtacaaagc tccgaagaat
      841 atattcagct attgaagaag cttattaggt cgcctagcat acctcatcag tattggctta
      901 cgcttcagta tttgttaaaa catttcttca agctctctca aacctccagc aaaaatctgt
      961 tgaatgcaag agtactctct gaaattttca gccctatgct tttcagattc tcagcagcca
     1021 gctctgataa tactgaaaac ctcataaaag ttatagaaat tttaatctca actgaatgga
     1081 atgaacgaca gcctgcacca gcactgcctc ctaaaccacc aaaacctact actgtagcca
     1141 acaacggtat gaataacaat atgtccttac aagatgctga atggtactgg ggagatatct
     1201 cgagggaaga agtgaatgaa aaacttcgag atacagcaga cgggaccttt ttggtacgag
     1261 atgcgtctac taaaatgcat ggtgattata ctcttacact aaggaaaggg ggaaataaca
     1321 aattaatcaa aatatttcat cgagatggga aatatggctt ctctgaccca ttaaccttca
     1381 gttctgtggt tgaattaata aaccactacc ggaatgaatc tctagctcag tataatccca
     1441 aattggatgt gaaattactt tatccagtat ccaaatacca acaggatcaa gttgtcaaag
     1501 aagataatat tgaagctgta gggaaaaaat tacatgaata taacactcag tttcaagaaa
     1561 aaagtcgaga atatgataga ttatatgaag aatatacccg cacatcccag gaaatccaaa
     1621 tgaaaaggac agctattgaa gcatttaatg aaaccataaa aatatttgaa gaacagtgcc
     1681 agacccaaga gcggtacagc aaagaataca tagaaaagtt taaacgtgaa ggcaatgaga
     1741 aagaaataca aaggattatg cataattatg ataagttgaa gtctcgaatc agtgaaatta
     1801 ttgacagtag aagaagattg gaagaagact tgaagaagca ggcagctgag tatcgagaaa
     1861 ttgacaaacg tatgaacagc attaaaccag accttatcca gctgagaaag acgagagacc
     1921 aatacttgat gtggttgact caaaaaggtg ttcggcaaaa gaagttgaac gagtggttgg
     1981 gcaatgaaaa cactgaagac caatattcac tggtggaaga tgatgaagat ttgccccatc
     2041 atgatgagaa gacatggaat gttggaagca gcaaccgaaa caaagctgaa aacctgttgc
     2101 gagggaagcg agatggcact tttcttgtcc gggagagcag taaacagggc tgctatgcct
     2161 gctctgtagt ggtggacggc gaagtaaagc attgtgtcat aaacaaaaca gcaactggct
     2221 atggctttgc cgagccctat aacttgtaca gctctctgaa agaactggtg ctacattacc
     2281 aacacacctc ccttgtgcag cacaacgact ccctcaatgt cacactagcc tacccagtat
     2341 atgcacagca gaggcgataa tcaactttct tgtacaaagt tggcattata ggaaagcatt
     2401 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca
     2461 tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     2521 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2581 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2641 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2701 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2761 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2821 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2881 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2941 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     3001 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     3061 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     3121 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     3181 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     3241 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     3301 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     3361 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3421 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3481 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3541 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3601 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3661 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3721 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3781 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3841 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3901 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3961 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4021 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4081 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     4141 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4201 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4261 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4321 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4381 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4441 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4501 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4561 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4621 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4681 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4741 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4801 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4861 ctctcgcg
//
