LOCUS       sequence-125908-        4079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3R3 [NM_003629.3] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4079)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E175 Hs.PIK3R3
FEATURES             Location/Qualifiers
     source          1..4079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1570..1669)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2046..2837
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2930..3518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3419..3438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3672..3689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3848..3875)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3967..4053)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgta caatacggtg tggagtatgg accgcgatga cgcagactgg agggaggtga
      241 tgatgcccta ttcgacagaa ctgatatttt atattgaaat ggatcctcca gctcttccac
      301 caaagccacc taagccaatg acttcagcag ttccaaatgg aatgaaggac agttctgttt
      361 ctcttcagga tgcagaatgg tactgggggg atatttcaag ggaggaggta aatgacaaat
      421 tgcgggatat gccagatggg accttcttgg tccgagatgc ctcaacaaaa atgcagggag
      481 attatacttt gactttgcgg aagggaggca ataataagtt aataaagatc tatcaccggg
      541 atggtaaata tggcttttct gatcctctga catttaattc cgtggtggag ctcattaacc
      601 actatcacca tgaatctctt gctcagtaca atcccaaact tgatgtgaag ctgatgtacc
      661 cagtgtccag ataccaacag gatcagttgg taaaagaaga taatattgat gcagtaggta
      721 aaaaactgca agaataccac tctcagtatc aggagaagag taaagagtat gataggctgt
      781 atgaagaata tactagaaca tcccaggaaa tacagatgaa gaggactgca atagaagctt
      841 ttaatgaaac aattaaaata tttgaagagc agtgtcacac acaagaacaa catagcaaag
      901 aatatattga gcgatttcgc agagagggga atgaaaagga gattgaacga attatgatga
      961 attatgataa attgaaatca cgtctgggtg agattcatga tagcaaaatg cgtctagagc
     1021 aggatttgaa gaatcaagct ttggacaacc gagaaataga taaaaaaatg aatagcatca
     1081 aacctgacct gatccagctg cgaaagatcc gagatcaaca ccttgtatgg ctcaatcaca
     1141 aaggagtgag acagaaacgc ctgaatgtct ggctgggaat taagaatgag gatgctgatg
     1201 agaactattt tatcaatgag gaagatgaaa acctgcccca ttatgatgag aaaacctggt
     1261 ttgttgagga tatcaatcga gtacaagcag aggacttgct ttatgggaaa cctgatggtg
     1321 cattcttaat tcgtgagagt agcaagaaag gatgctatgc ttgctctgtg gtggccgatg
     1381 gggaagtgaa gcactgtgtg atctacagca ctgctcgggg ctatggcttt gcagagccct
     1441 acaacctgta cagctctctg aaggagctag tgctccatta ccagcagaca tccttggttc
     1501 agcacaacga ctccctcaac gtcaggcttg cctaccctgt tcatgcacag atgccctcgc
     1561 tttgcagata atcaactttc ttgtacaaag ttggcattat aggaaagcat tgcttatcaa
     1621 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc atgcaagctt
     1681 agatcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1741 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     1801 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     1861 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1921 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1981 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2041 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2101 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2161 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2221 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2281 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2341 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2401 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2461 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2521 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2581 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2641 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2701 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2761 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2821 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     2881 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3061 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3301 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3601 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3661 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3721 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3781 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3841 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3901 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     3961 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4021 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcg
//
