LOCUS       sequence-125909-        4076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3R3 [NM_003629.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4076)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E176 Hs.PIK3R3-nostop
FEATURES             Location/Qualifiers
     source          1..4076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1567..1666)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2043..2834
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2927..3515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3416..3435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3669..3686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3845..3872)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3964..4050)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgta caatacggtg tggagtatgg accgcgatga cgcagactgg agggaggtga
      241 tgatgcccta ttcgacagaa ctgatatttt atattgaaat ggatcctcca gctcttccac
      301 caaagccacc taagccaatg acttcagcag ttccaaatgg aatgaaggac agttctgttt
      361 ctcttcagga tgcagaatgg tactgggggg atatttcaag ggaggaggta aatgacaaat
      421 tgcgggatat gccagatggg accttcttgg tccgagatgc ctcaacaaaa atgcagggag
      481 attatacttt gactttgcgg aagggaggca ataataagtt aataaagatc tatcaccggg
      541 atggtaaata tggcttttct gatcctctga catttaattc cgtggtggag ctcattaacc
      601 actatcacca tgaatctctt gctcagtaca atcccaaact tgatgtgaag ctgatgtacc
      661 cagtgtccag ataccaacag gatcagttgg taaaagaaga taatattgat gcagtaggta
      721 aaaaactgca agaataccac tctcagtatc aggagaagag taaagagtat gataggctgt
      781 atgaagaata tactagaaca tcccaggaaa tacagatgaa gaggactgca atagaagctt
      841 ttaatgaaac aattaaaata tttgaagagc agtgtcacac acaagaacaa catagcaaag
      901 aatatattga gcgatttcgc agagagggga atgaaaagga gattgaacga attatgatga
      961 attatgataa attgaaatca cgtctgggtg agattcatga tagcaaaatg cgtctagagc
     1021 aggatttgaa gaatcaagct ttggacaacc gagaaataga taaaaaaatg aatagcatca
     1081 aacctgacct gatccagctg cgaaagatcc gagatcaaca ccttgtatgg ctcaatcaca
     1141 aaggagtgag acagaaacgc ctgaatgtct ggctgggaat taagaatgag gatgctgatg
     1201 agaactattt tatcaatgag gaagatgaaa acctgcccca ttatgatgag aaaacctggt
     1261 ttgttgagga tatcaatcga gtacaagcag aggacttgct ttatgggaaa cctgatggtg
     1321 cattcttaat tcgtgagagt agcaagaaag gatgctatgc ttgctctgtg gtggccgatg
     1381 gggaagtgaa gcactgtgtg atctacagca ctgctcgggg ctatggcttt gcagagccct
     1441 acaacctgta cagctctctg aaggagctag tgctccatta ccagcagaca tccttggttc
     1501 agcacaacga ctccctcaac gtcaggcttg cctaccctgt tcatgcacag atgccctcgc
     1561 tttgcagatc aactttcttg tacaaagttg gcattatagg aaagcattgc ttatcaattt
     1621 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgcatg caagcttaga
     1681 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1741 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1801 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1861 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1921 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1981 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2041 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2101 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2161 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2221 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2281 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2341 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2401 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2461 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2521 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2581 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2641 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2701 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2761 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2821 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     2881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3061 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3301 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3721 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3781 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3841 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3901 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3961 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     4021 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcg
//
