LOCUS       sequence-125910-        6770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3R4 [NM_014602.2] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6770)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E177 Hs.PIK3R4
FEATURES             Location/Qualifiers
     source          1..6770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4261..4360)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4737..5528
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5621..6209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6110..6129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6363..6380
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6539..6566)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6658..6744)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggg aaatcagctt gctggcattg ctccctccca gatcctttct gtagagagtt
      241 atttttcaga tattcatgac tttgaatatg ataaaagcct ggggagtact cggtttttta
      301 aagttgctcg agccaagcac cgagaaggcc tggtcgttgt gaaggttttt gcaattcagg
      361 atcccacatt gcctttaacc agctataaac aagagctgga ggaactgaaa atcaggctta
      421 attctgcaca gaattgtcta cctttccaga aagcatcaga aaaagcatct gagaaagcag
      481 ctatgctctt taggcagtat gtgcgagaca atctctatga tcgcatcagt acccgtccat
      541 tcttgaataa cattgagaag cgctggattg ctttccagat cctgacagct gtggaccaag
      601 cacacaaatc tggagttcgt catggggaca tcaagactga gaatgtgatg gtcaccagtt
      661 ggaattgggt tcttctaact gattttgcca gttttaagcc cacttatctt ccagaagaca
      721 acccggcaga tttcaattat ttctttgaca catcacggag gagaacttgc tatattgctc
      781 ctgaacgttt tgttgatggt gggatgtttg ccactgagtt agaatatatg agagatcctt
      841 caactccgct tgtagactta aatagcaatc agagaacaag aggagagttg aagagagcaa
      901 tggacatctt ttcagcaggt tgtgtgatag ctgagctttt tacagaaggt gtaccattat
      961 ttgatctctc tcaacttttg gcttatagaa atggacattt tttccctgaa caagtgctaa
     1021 ataaaattga agatcacagt atcagagaat tggtaactca gatgattcac cgtgagccag
     1081 ataaacgttt agaggcagaa gattacttaa aacagcagcg tggcaatgcc tttcctgaaa
     1141 tattttacac ttttcttcag ccctacatgg cccagtttgc caaggaaacg tttctttctg
     1201 cagatgagcg tattctggtt atacggaagg atttgggcaa cattattcac aatctctgtg
     1261 gacatgatct gccagaaaaa gccgaaggag agcctaagga aaatgggctg gttatcttgg
     1321 tatctgttat aacatcctgc ctacagaccc ttaaatactg tgattccaaa ctagctgctt
     1381 tggaactgat tcttcatttg gctccaagat taagtgttga aatccttttg gatcgtatta
     1441 ctccatatct tttgcatttc agcaatgact ctgttcctag ggtgagggct gaagccttga
     1501 ggacgttgac caaagttctt gctctcgtca aagaggttcc tcgtaatgat atcaatattt
     1561 atccggaata cattctgcca ggcatagccc acttagccca agatgatgct actatcgtta
     1621 gactagccta tgctgaaaac atagctctgc tggcagaaac agctctgaga ttcctggaat
     1681 tagtacagtt aaaaaatctt aatatggaaa atgaccccaa taatgaagaa atagatgagg
     1741 ttacacatcc aaatggaaat tatgacacag agctccaagc cttacatgaa atggtccagc
     1801 agaaagttgt tactttgcta agtgaccctg aaaatattgt aaaacaaacc ttgatggaaa
     1861 atggaataac acggctgtgt gtattctttg gacgtcagaa agccaacgat gttttgttgt
     1921 cccacatgat tactttccta aatgataaga atgattggca tctacgtgga gcattttttg
     1981 atagtatagt tggtgttgct gcctatgttg gctggcaaag ctcctcaatt ctcaagcctc
     2041 tgctgcaaca aggtcttagt gatgctgagg aatttgtcat tgtgaaagct ctttatgccc
     2101 ttacttgtat gtgccagtta ggactgctac aaaaacccca tgtttacgaa tttgccagtg
     2161 atattgcccc cttcctgtgt catcccaatt tatggatacg ttatggtgcc gtgggattta
     2221 tcacagtggt agctcgtcaa ataagtacag ctgatgtcta ctgtaaactg atgccttatc
     2281 ttgacccata tattacccaa ccaataatac agattgaaag aaaacttgtt ctgctcagtg
     2341 ttttaaagga accagtaagt cgttctatat ttgattatgc tttgaggtct aaagatatta
     2401 ctagcttgtt cagacatctt cacatgcgtc agaagaaacg aaatggttct cttcccgact
     2461 gccctccgcc agaggatcct gccatagcac agcttctgaa gaagttgctc tcacagggaa
     2521 tgacagagga agaggaagac aaacttctgg cactgaaaga cttcatgatg aaatctaata
     2581 aagcaaaggc caatatagtg gaccagagcc atcttcatga tagtagtcag aaaggtgtaa
     2641 ttgacttggc agctttaggc ataactggga gacaagttga tcttgttaaa accaaacaag
     2701 aaccagatga caaacgggcc agaaaacatg taaaacaaga ctcaaatgta aatgaagaat
     2761 ggaaaagcat gtttgggtca ctggacccac caaacatgcc acaggcccta cctaaaggga
     2821 gtgatcagga ggtgattcag actgggaaac ctcctcgttc cgagtcctct gctggcattt
     2881 gtgtcccttt gtcaacttct tcacaggttc cagaagtgac aactgtccaa aataaaaaac
     2941 cagtaatacc ggttttaagt agtacaatct taccatccac ctatcagatt cgaattacaa
     3001 cttgtaaaac tgaacttcag caactcatcc agcaaaagcg ggagcagtgc aatgctgaga
     3061 gaatagctaa gcagatgatg gaaaatgctg aatgggagag taaaccacca ccacctggat
     3121 ggcgtcctaa agggctgtta gttgcccatc ttcatgagca taaatctgct gtgaatcgaa
     3181 ttagagtctc tgatgaacac tcactttttg caacatgttc aaatgatggc acagtgaaaa
     3241 tctggaacag tcaaaagatg gaggggaaga ccaccactac cagatctatt cttacataca
     3301 gccgaattgg aggacgagtc aagacgctca cattctgcca aggctcccac tatttagcca
     3361 tagcatctga taatggtgct gtccagcttc ttggaattga ggcttctaag ctgcccaagt
     3421 ctcctaaaat ccatcctcta caaagcagaa ttctagatca gaaggaggac ggttgtgttg
     3481 tggatatgca tcacttcaac tctggagcac agtctgttct tgcctatgcc actgtgaatg
     3541 gctctctggt tggctgggac cttaggtctt caagcaatgc gtggacttta aagcatgatt
     3601 taaagtcggg cctcatcact tcctttgctg tggacatcca ccaatgctgg ctctgcattg
     3661 gtacaagcag tggtaccatg gcttgttggg acatgaggtt ccagttgcca atttcaagtc
     3721 actgtcatcc ttccagggct cgaatcagac gcctctcaat gcaccctctg tatcagtcct
     3781 gggtgattgc agctgttcag ggcaacaacg aagtgtccat gtgggacatg gagactggtg
     3841 acagaagatt tactctctgg gccagcagtg caccaccact ttctgaatta cagccttctc
     3901 ctcatagcgt ccatggtatc tactgtagtc ctgcagatgg aaatcctatc ctactaacag
     3961 ctggctcaga tatgaaaata aggttttggg acttggctta cccagaaagg tcctatgttg
     4021 ttgcaggaag tactagttcc ccatctgtgt cctactacag gaaaataatt gaaggcactg
     4081 aagttgtcca ggaaattcag aataagcaga aagtaggacc aagtgatgac acccctcgaa
     4141 ggggcccaga gtccctgccc gtgggacatc atgacatcat cactgatgtc gccacattcc
     4201 agaccacaca gggcttcatc gtaactgctt ctagagatgg gattgtgaag gtgtggaaat
     4261 aatcaacttt cttgtacaaa gttggcatta taggaaagca ttgcttatca atttgttgca
     4321 acgaacaggt cactatcagt caaaataaaa tcattatttg catgcaagct tagatcctct
     4381 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     4441 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     4501 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     4561 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     4621 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     4681 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     4741 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     4801 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     4861 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     4921 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     4981 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     5041 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     5101 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     5161 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     5221 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     5281 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     5341 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     5401 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     5461 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     5521 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     5581 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5641 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5701 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5761 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5821 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5881 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5941 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6001 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6061 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6121 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6181 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6241 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6301 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6361 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6421 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6481 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     6541 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     6601 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     6661 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     6721 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
//
