LOCUS       sequence-125925-        4370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PRKAA1 [NM_006251.5] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4370)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E192 Hs.PRKAA1-nostop
FEATURES             Location/Qualifiers
     source          1..4370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1861..1960)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2337..3128
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3221..3809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3710..3729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3963..3980
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4139..4166)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4258..4344)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcg cagactcagt tcctggagaa agatggcgac agccgagaag cagaaacacg
      241 acgggcgggt gaagatcggc cactacattc tgggtgacac gctgggggtc ggcaccttcg
      301 gcaaagtgaa ggttggcaaa catgaattga ctgggcataa agtagctgtg aagatactca
      361 atcgacagaa gattcggagc cttgatgtgg taggaaaaat ccgcagagaa attcagaacc
      421 tcaagctttt caggcatcct catataatta aactgtacca ggtcatcagt acaccatctg
      481 atattttcat ggtgatggaa tatgtctcag gaggagagct atttgattat atctgtaaga
      541 atggaaggct ggatgaaaaa gaaagtcggc gtctgttcca acagatcctt tctggtgtgg
      601 attattgtca caggcatatg gtggtccata gagatttgaa acctgaaaat gtcctgcttg
      661 atgcacacat gaatgcaaag atagctgatt ttggtctttc aaacatgatg tcagatggtg
      721 aatttttaag aacaagttgt ggctcaccca actatgctgc accagaagta atttcaggaa
      781 gattgtatgc aggcccagag gtagatatat ggagcagtgg ggttattctc tatgctttat
      841 tatgtggaac ccttccattt gatgatgacc atgtgccaac tctttttaag aagatatgtg
      901 atgggatctt ctatacccct caatatttaa atccttctgt gattagcctt ttgaaacata
      961 tgctgcaggt ggatcccatg aagagggcca caatcaaaga tatcagggaa catgaatggt
     1021 ttaaacagga ccttccaaaa tatctctttc ctgaggatcc atcatatagt tcaaccatga
     1081 ttgatgatga agccttaaaa gaagtatgtg aaaagtttga gtgctcagaa gaggaagttc
     1141 tcagctgtct ttacaacaga aatcaccagg atcctttggc agttgcctac catctcataa
     1201 tagataacag gagaataatg aatgaagcca aagatttcta tttggcgaca agcccacctg
     1261 attcttttct tgatgatcat cacctgactc ggccccatcc tgaaagagta ccattcttgg
     1321 ttgctgaaac accaagggca cgccataccc ttgatgaatt aaatccacag aaatccaaac
     1381 accaaggtgt aaggaaagca aaatggcatt taggaattag aagtcaaagt cgaccaaatg
     1441 atattatggc agaagtatgt agagcaatca aacaattgga ttatgaatgg aaggttgtaa
     1501 acccatatta tttgcgtgta cgaaggaaga atcctgtgac aagcacttac tccaaaatga
     1561 gtctacagtt ataccaagtg gatagtagaa cttatctact ggatttccgt agtattgatg
     1621 atgaaattac agaagccaaa tcagggactg ctactccaca gagatcggga tcagttagca
     1681 actatcgatc ttgccaaagg agtgattcag atgctgaggc tcaaggaaaa tcctcagaag
     1741 tttctcttac ctcatctgtg acctcacttg actcttctcc tgttgaccta actccaagac
     1801 ctggaagtca cacaatagaa ttttttgaga tgtgtgcaaa tctaattaaa attcttgcac
     1861 aatcaacttt cttgtacaaa gttggcatta taggaaagca ttgcttatca atttgttgca
     1921 acgaacaggt cactatcagt caaaataaaa tcattatttg catgcaagct tagatcctct
     1981 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     2041 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     2101 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     2161 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     2221 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     2281 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     2341 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     2401 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     2461 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     2521 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     2581 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     2641 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     2701 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     2761 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     2821 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     2881 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     2941 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     3001 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     3061 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     3121 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     3181 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3241 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3301 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3361 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3421 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3481 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3541 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3601 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3661 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3721 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3781 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3841 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3901 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3961 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4021 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4081 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     4141 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     4201 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     4261 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     4321 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
//
