LOCUS       sequence-125926-        4352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PRKAA2 [NM_006252.3] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4352)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E193 Hs.PRKAA2
FEATURES             Location/Qualifiers
     source          1..4352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1843..1942)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2319..3110
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3203..3791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3692..3711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3945..3962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4121..4148)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4240..4326)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc tgagaagcag aagcacgacg ggcgggtgaa gatcggacac tacgtgctgg
      241 gcgacacgct gggcgtcggc accttcggca aagtgaagat tggagaacat caattaacag
      301 gccataaagt ggcagttaaa atcttaaata gacagaagat tcgcagttta gatgttgttg
      361 gaaaaataaa acgagaaatt caaaatctaa aactctttcg tcatcctcat attatcaaac
      421 tataccaggt gatcagcact ccaacagatt tttttatggt aatggaatat gtgtctggag
      481 gtgaattatt tgactacatc tgtaagcatg gacgggttga agagatggaa gccaggcggc
      541 tctttcagca gattctgtct gctgtggatt actgtcatag gcatatggtt gttcatcgag
      601 acctgaaacc agagaatgtc ctgttggatg cacacatgaa tgccaagata gccgatttcg
      661 gattatctaa tatgatgtca gatggtgaat ttctgagaac tagttgcgga tctccaaatt
      721 atgcagcacc tgaagtcatc tcaggcagat tgtatgcagg tcctgaagtt gatatctgga
      781 gctgtggtgt tatcttgtat gctcttcttt gtggcaccct cccatttgat gatgagcatg
      841 tacctacgtt atttaagaag atccgagggg gtgtctttta tatcccagaa tatctcaatc
      901 gttctgtcgc cactctcctg atgcatatgc tgcaggttga cccactgaaa cgagcaacta
      961 tcaaagacat aagagagcat gaatggttta aacaagattt gcccagttac ttatttcctg
     1021 aagacccttc ctatgatgct aacgtcattg atgatgaggc tgtgaaagaa gtgtgtgaaa
     1081 aatttgaatg tacagaatca gaagtaatga acagtttata tagtggtgac cctcaagacc
     1141 agcttgcagt ggcttatcat cttatcattg acaatcggag aataatgaac caagccagtg
     1201 agttctacct cgcctctagt cctccatctg gttcttttat ggatgatagt gccatgcata
     1261 ttcccccagg cctgaaacct catccagaaa ggatgccacc tcttatagca gacagcccca
     1321 aagcaagatg tccattggat gcactgaata cgactaagcc caaatcttta gctgtgaaaa
     1381 aagccaagtg gcatcttgga atccgaagtc agagcaaacc gtatgacatt atggctgaag
     1441 tttaccgagc tatgaagcag ctggattttg aatggaaggt agtgaatgca taccatcttc
     1501 gtgtaagaag aaaaaatcca gtgactggca attacgtgaa aatgagctta caactttacc
     1561 tggttgataa caggagctat cttttggact ttaaaagcat tgatgatgaa gtagtggagc
     1621 agagatctgg ttcctcaaca cctcagcgtt cctgttctgc tgctggctta cacagaccaa
     1681 gatcaagttt tgattccaca actgcagaga gccattcact ttctggctct ctcactggct
     1741 ctttgaccgg aagcacattg tcttcagttt cacctcgcct gggcagtcac accatggatt
     1801 tttttgaaat gtgtgccagt ctgattacta ctttagcccg ttaatcaact ttcttgtaca
     1861 aagttggcat tataggaaag cattgcttat caatttgttg caacgaacag gtcactatca
     1921 gtcaaaataa aatcattatt tgcatgcaag cttagatcct ctagaccagc caggacagaa
     1981 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     2041 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     2101 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     2161 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     2221 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     2281 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     2341 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     2401 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     2461 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     2521 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     2581 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     2641 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     2701 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     2761 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     2821 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     2881 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     2941 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     3001 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     3061 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     3121 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     3181 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     3361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     3601 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3661 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3721 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3781 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3841 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3901 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3961 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4021 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     4081 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     4141 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     4201 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     4261 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     4321 tatttgatgc ctggcagttc cctactctcg cg
//
