LOCUS       sequence-125927-        4349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PRKAA2 [NM_006252.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4349)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E194 Hs.PRKAA2-nostop
FEATURES             Location/Qualifiers
     source          1..4349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1840..1939)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2316..3107
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3200..3788
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3689..3708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3942..3959
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4118..4145)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4237..4323)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc tgagaagcag aagcacgacg ggcgggtgaa gatcggacac tacgtgctgg
      241 gcgacacgct gggcgtcggc accttcggca aagtgaagat tggagaacat caattaacag
      301 gccataaagt ggcagttaaa atcttaaata gacagaagat tcgcagttta gatgttgttg
      361 gaaaaataaa acgagaaatt caaaatctaa aactctttcg tcatcctcat attatcaaac
      421 tataccaggt gatcagcact ccaacagatt tttttatggt aatggaatat gtgtctggag
      481 gtgaattatt tgactacatc tgtaagcatg gacgggttga agagatggaa gccaggcggc
      541 tctttcagca gattctgtct gctgtggatt actgtcatag gcatatggtt gttcatcgag
      601 acctgaaacc agagaatgtc ctgttggatg cacacatgaa tgccaagata gccgatttcg
      661 gattatctaa tatgatgtca gatggtgaat ttctgagaac tagttgcgga tctccaaatt
      721 atgcagcacc tgaagtcatc tcaggcagat tgtatgcagg tcctgaagtt gatatctgga
      781 gctgtggtgt tatcttgtat gctcttcttt gtggcaccct cccatttgat gatgagcatg
      841 tacctacgtt atttaagaag atccgagggg gtgtctttta tatcccagaa tatctcaatc
      901 gttctgtcgc cactctcctg atgcatatgc tgcaggttga cccactgaaa cgagcaacta
      961 tcaaagacat aagagagcat gaatggttta aacaagattt gcccagttac ttatttcctg
     1021 aagacccttc ctatgatgct aacgtcattg atgatgaggc tgtgaaagaa gtgtgtgaaa
     1081 aatttgaatg tacagaatca gaagtaatga acagtttata tagtggtgac cctcaagacc
     1141 agcttgcagt ggcttatcat cttatcattg acaatcggag aataatgaac caagccagtg
     1201 agttctacct cgcctctagt cctccatctg gttcttttat ggatgatagt gccatgcata
     1261 ttcccccagg cctgaaacct catccagaaa ggatgccacc tcttatagca gacagcccca
     1321 aagcaagatg tccattggat gcactgaata cgactaagcc caaatcttta gctgtgaaaa
     1381 aagccaagtg gcatcttgga atccgaagtc agagcaaacc gtatgacatt atggctgaag
     1441 tttaccgagc tatgaagcag ctggattttg aatggaaggt agtgaatgca taccatcttc
     1501 gtgtaagaag aaaaaatcca gtgactggca attacgtgaa aatgagctta caactttacc
     1561 tggttgataa caggagctat cttttggact ttaaaagcat tgatgatgaa gtagtggagc
     1621 agagatctgg ttcctcaaca cctcagcgtt cctgttctgc tgctggctta cacagaccaa
     1681 gatcaagttt tgattccaca actgcagaga gccattcact ttctggctct ctcactggct
     1741 ctttgaccgg aagcacattg tcttcagttt cacctcgcct gggcagtcac accatggatt
     1801 tttttgaaat gtgtgccagt ctgattacta ctttagcccg ttcaactttc ttgtacaaag
     1861 ttggcattat aggaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1921 aaaataaaat cattatttgc atgcaagctt agatcctcta gaccagccag gacagaaatg
     1981 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     2041 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     2101 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     2161 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     2221 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     2281 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     2341 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     2401 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     2461 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     2521 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2581 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2641 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2701 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2761 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2821 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2881 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2941 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     3001 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     3061 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     3121 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3181 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3241 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3301 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3361 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3421 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3481 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3541 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     3601 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3661 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3721 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3781 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3841 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3901 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3961 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4021 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     4081 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     4141 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     4201 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     4261 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4321 ttgatgcctg gcagttccct actctcgcg
//
