LOCUS       sequence-125928-        3506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PRKAB1 [NM_006253.4] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3506)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E195 Hs.PRKAB1
FEATURES             Location/Qualifiers
     source          1..3506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(997..1096)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1473..2264
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2357..2945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2846..2865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3099..3116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3275..3302)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3394..3480)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggg caataccagc agtgagcgcg ccgcgctgga gcggcatggt ggccataaga
      241 cgccccggag ggacagctcg gggggcacca aggacgggga caggcccaag atcctgatgg
      301 acagccccga agacgccgac ctcttccact ccgaggaaat caaggcacca gagaaggagg
      361 aattcctggc ctggcagcat gatctggaag tgaatgataa agctcccgcc caggctcggc
      421 caacggtgtt tcgatggacg gggggcggaa aggaagttta cttatctggg tccttcaaca
      481 actggagtaa acttcccctc accagaagcc acaataactt tgtagccatc ctggatctgc
      541 cggaaggaga gcatcagtac aagttctttg tggatggtca gtggacgcac gacccttccg
      601 agcccatagt aaccagccag cttggcacag ttaacaacat cattcaagtg aagaaaactg
      661 actttgaagt atttgatgct ttaatggtgg attcccaaaa gtgctccgat gtgtctgagc
      721 tgtccagttc tcccccagga ccctaccatc aggagcccta cgtctgcaaa cccgaagagc
      781 gctttcgggc accccctatt ctccccccac atctcctcca ggtcatcctg aacaaggaca
      841 cggggatttc ctgtgatcca gctttgcttc ctgagcccaa tcacgtcatg ctgaaccacc
      901 tatacgcgct gtctatcaag gatggagtga tggtgctcag cgcaacccac cggtacaaga
      961 agaagtacgt caccaccttg ttatacaagc ccatataatc aactttcttg tacaaagttg
     1021 gcattatagg aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     1081 ataaaatcat tatttgcatg caagcttaga tcctctagac cagccaggac agaaatgcct
     1141 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     1201 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     1261 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     1321 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     1381 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     1441 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     1501 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     1561 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     1621 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     1681 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     1741 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     1801 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     1861 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     1921 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     1981 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2041 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2101 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     2161 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     2221 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     2281 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     2341 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2401 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2461 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2521 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2581 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2641 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2701 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     2761 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2821 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2881 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2941 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3001 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3061 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3121 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3181 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     3241 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3301 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3361 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3421 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     3481 atgcctggca gttccctact ctcgcg
//
