LOCUS       sequence-125935-        3677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PRKAG2 [NM_024429.1] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3677)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E202 Hs.PRKAG2-nostop
FEATURES             Location/Qualifiers
     source          1..3677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1168..1267)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1644..2435
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2528..3116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3017..3036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3270..3287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3446..3473)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3565..3651)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgct ggagaagctg gagttcgagg acgaagcagt agaagactca gaaagtggtg
      241 tttacatgcg attcatgagg tcacacaagt gttatgacat cgttccaacc agttcaaagc
      301 ttgttgtctt tgatactaca ttacaagtta aaaaggcctt ctttgctttg gtagccaacg
      361 gtgtcagagc agcgccactg tgggagagta aaaaacaaag ttttgtagga atgctaacaa
      421 ttacagattt cataaatata ctacatagat actataaatc acctatggta cagatttatg
      481 aattagagga acataaaatt gaaacatgga gggagcttta tttacaagaa acatttaagc
      541 ctttagtgaa tatatctcca gatgcaagcc tcttcgatgc tgtatactcc ttgatcaaaa
      601 ataaaatcca cagattgccc gttattgacc ctatcagtgg gaatgcactt tatatactta
      661 cccacaaaag aatcctcaag ttcctccagc tttttatgtc tgatatgcca aagcctgcct
      721 tcatgaagca gaacctggat gagcttggaa taggaacgta ccacaacatt gccttcatac
      781 atccagacac tcccatcatc aaagccttga acatatttgt ggaaagacga atatcagctc
      841 tgcctgttgt ggatgagtca ggaaaagttg tagatattta ttccaaattt gatgtaatta
      901 atcttgctgc tgagaaaaca tacaataacc tagatatcac ggtgacccag gcccttcagc
      961 accgttcaca gtattttgaa ggtgttgtga agtgcaataa gctggaaata ctggagacca
     1021 tcgtggacag aatagtaaga gctgaggtcc atcggctggt ggtggtaaat gaagcagata
     1081 gtattgtggg tattatttcc ctgtcggaca ttctgcaagc cctgatcctc acaccagcag
     1141 gtgccaaaca aaaggagaca gaaacggagt caactttctt gtacaaagtt ggcattatag
     1201 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1261 ttatttgcat gcaagcttag atcctctaga ccagccagga cagaaatgcc tcgacttcgc
     1321 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     1381 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     1441 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1501 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1561 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1621 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     1681 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1741 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     1801 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1861 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     1921 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1981 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     2041 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2101 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2161 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2221 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2281 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2341 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     2401 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     2461 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2641 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2881 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     2941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3361 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     3421 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     3481 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     3541 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     3601 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     3661 agttccctac tctcgcg
//
