LOCUS       sequence-125946-        4640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RAF1 [NM_002880.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4640)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E213 Hs.RAF1
FEATURES             Location/Qualifiers
     source          1..4640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2131..2230)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2607..3398
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3491..4079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3980..3999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4233..4250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4409..4436)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4528..4614)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga gcacatacag ggagcttgga agacgatcag caatggtttt ggattcaaag
      241 atgccgtgtt tgatggctcc agctgcatct ctcctacaat agttcagcag tttggctatc
      301 agcgccgggc atcagatgat ggcaaactca cagatccttc taagacaagc aacactatcc
      361 gtgttttctt gccgaacaag caaagaacag tggtcaatgt gcgaaatgga atgagcttgc
      421 atgactgcct tatgaaagca ctcaaggtga ggggcctgca accagagtgc tgtgcagtgt
      481 tcagacttct ccacgaacac aaaggtaaaa aagcacgctt agattggaat actgatgctg
      541 cgtctttgat tggagaagaa cttcaagtag atttcctgga tcatgttccc ctcacaacac
      601 acaactttgc tcggaagacg ttcctgaagc ttgccttctg tgacatctgt cagaaattcc
      661 tgctcaatgg atttcgatgt cagacttgtg gctacaaatt tcatgagcac tgtagcacca
      721 aagtacctac tatgtgtgtg gactggagta acatcagaca actcttattg tttccaaatt
      781 ccactattgg tgatagtgga gtcccagcac taccttcttt gactatgcgt cgtatgcgag
      841 agtctgtttc caggatgcct gttagttctc agcacagata ttctacacct cacgccttca
      901 cctttaacac ctccagtccc tcatctgaag gttccctctc ccagaggcag aggtcgacat
      961 ccacacctaa tgtccacatg gtcagcacca ccctgcctgt ggacagcagg atgattgagg
     1021 atgcaattcg aagtcacagc gaatcagcct caccttcagc cctgtccagt agccccaaca
     1081 atctgagccc aacaggctgg tcacagccga aaacccccgt gccagcacaa agagagcggg
     1141 caccagtatc tgggacccag gagaaaaaca aaattaggcc tcgtggacag agagattcaa
     1201 gctattattg ggaaatagaa gccagtgaag tgatgctgtc cactcggatt gggtcaggct
     1261 cttttggaac tgtttataag ggtaaatggc acggagatgt tgcagtaaag atcctaaagg
     1321 ttgtcgaccc aaccccagag caattccagg ccttcaggaa tgaggtggct gttctgcgca
     1381 aaacacggca tgtgaacatt ctgcttttca tggggtacat gacaaaggac aacctggcaa
     1441 ttgtgaccca gtggtgcgag ggcagcagcc tctacaaaca cctgcatgtc caggagacca
     1501 agtttcagat gttccagcta attgacattg cccggcagac ggctcaggga atggactatt
     1561 tgcatgcaaa gaacatcatc catagagaca tgaaatccaa caatatattt ctccatgaag
     1621 gcttaacagt gaaaattgga gattttggtt tggcaacagt aaagtcacgc tggagtggtt
     1681 ctcagcaggt tgaacaacct actggctctg tcctctggat ggccccagag gtgatccgaa
     1741 tgcaggataa caacccattc agtttccagt cggatgtcta ctcctatggc atcgtattgt
     1801 atgaactgat gacgggggag cttccttatt ctcacatcaa caaccgagat cagatcatct
     1861 tcatggtggg ccgaggatat gcctccccag atcttagtaa gctatataag aactgcccca
     1921 aagcaatgaa gaggctggta gctgactgtg tgaagaaagt aaaggaagag aggcctcttt
     1981 ttccccagat cctgtcttcc attgagctgc tccaacactc tctaccgaag atcaaccgga
     2041 gcgcttccga gccatccttg catcgggcag cccacactga ggatatcaat gcttgcacgc
     2101 tgaccacgtc cccgaggctg cctgtcttct aatcaacttt cttgtacaaa gttggcatta
     2161 taggaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2221 tcattatttg catgcaagct tagatcctct agaccagcca ggacagaaat gcctcgactt
     2281 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     2341 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     2401 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2461 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2521 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2581 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2641 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2701 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2761 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2821 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2881 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2941 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     3001 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     3061 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3121 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     3181 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3241 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3301 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     3361 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3421 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3481 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3541 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3601 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3661 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3721 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3781 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3841 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     3901 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3961 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4021 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4081 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4141 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4201 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4261 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4321 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4381 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4441 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4501 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4561 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     4621 ggcagttccc tactctcgcg
//
