LOCUS       sequence-125947-        4637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RAF1 [NM_002880.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4637)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E214 Hs.RAF1-nostop
FEATURES             Location/Qualifiers
     source          1..4637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2128..2227)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2604..3395
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3488..4076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3977..3996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4230..4247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4406..4433)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4525..4611)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga gcacatacag ggagcttgga agacgatcag caatggtttt ggattcaaag
      241 atgccgtgtt tgatggctcc agctgcatct ctcctacaat agttcagcag tttggctatc
      301 agcgccgggc atcagatgat ggcaaactca cagatccttc taagacaagc aacactatcc
      361 gtgttttctt gccgaacaag caaagaacag tggtcaatgt gcgaaatgga atgagcttgc
      421 atgactgcct tatgaaagca ctcaaggtga ggggcctgca accagagtgc tgtgcagtgt
      481 tcagacttct ccacgaacac aaaggtaaaa aagcacgctt agattggaat actgatgctg
      541 cgtctttgat tggagaagaa cttcaagtag atttcctgga tcatgttccc ctcacaacac
      601 acaactttgc tcggaagacg ttcctgaagc ttgccttctg tgacatctgt cagaaattcc
      661 tgctcaatgg atttcgatgt cagacttgtg gctacaaatt tcatgagcac tgtagcacca
      721 aagtacctac tatgtgtgtg gactggagta acatcagaca actcttattg tttccaaatt
      781 ccactattgg tgatagtgga gtcccagcac taccttcttt gactatgcgt cgtatgcgag
      841 agtctgtttc caggatgcct gttagttctc agcacagata ttctacacct cacgccttca
      901 cctttaacac ctccagtccc tcatctgaag gttccctctc ccagaggcag aggtcgacat
      961 ccacacctaa tgtccacatg gtcagcacca ccctgcctgt ggacagcagg atgattgagg
     1021 atgcaattcg aagtcacagc gaatcagcct caccttcagc cctgtccagt agccccaaca
     1081 atctgagccc aacaggctgg tcacagccga aaacccccgt gccagcacaa agagagcggg
     1141 caccagtatc tgggacccag gagaaaaaca aaattaggcc tcgtggacag agagattcaa
     1201 gctattattg ggaaatagaa gccagtgaag tgatgctgtc cactcggatt gggtcaggct
     1261 cttttggaac tgtttataag ggtaaatggc acggagatgt tgcagtaaag atcctaaagg
     1321 ttgtcgaccc aaccccagag caattccagg ccttcaggaa tgaggtggct gttctgcgca
     1381 aaacacggca tgtgaacatt ctgcttttca tggggtacat gacaaaggac aacctggcaa
     1441 ttgtgaccca gtggtgcgag ggcagcagcc tctacaaaca cctgcatgtc caggagacca
     1501 agtttcagat gttccagcta attgacattg cccggcagac ggctcaggga atggactatt
     1561 tgcatgcaaa gaacatcatc catagagaca tgaaatccaa caatatattt ctccatgaag
     1621 gcttaacagt gaaaattgga gattttggtt tggcaacagt aaagtcacgc tggagtggtt
     1681 ctcagcaggt tgaacaacct actggctctg tcctctggat ggccccagag gtgatccgaa
     1741 tgcaggataa caacccattc agtttccagt cggatgtcta ctcctatggc atcgtattgt
     1801 atgaactgat gacgggggag cttccttatt ctcacatcaa caaccgagat cagatcatct
     1861 tcatggtggg ccgaggatat gcctccccag atcttagtaa gctatataag aactgcccca
     1921 aagcaatgaa gaggctggta gctgactgtg tgaagaaagt aaaggaagag aggcctcttt
     1981 ttccccagat cctgtcttcc attgagctgc tccaacactc tctaccgaag atcaaccgga
     2041 gcgcttccga gccatccttg catcgggcag cccacactga ggatatcaat gcttgcacgc
     2101 tgaccacgtc cccgaggctg cctgtcttct caactttctt gtacaaagtt ggcattatag
     2161 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     2221 ttatttgcat gcaagcttag atcctctaga ccagccagga cagaaatgcc tcgacttcgc
     2281 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     2341 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     2401 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2461 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2521 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2581 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     2641 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2701 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     2761 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2821 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2881 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2941 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3001 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3061 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3121 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3181 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3241 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3301 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     3361 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     3421 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3601 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3841 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     3901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4141 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4201 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4261 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4321 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     4381 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4441 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4501 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4561 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     4621 agttccctac tctcgcg
//
